Patents by Inventor Bettina Bommarius
Bettina Bommarius has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 8835136Abstract: Non-naturally occurring amine dehydrogenases (AmDH) and methods of use thereof the produce chiral amines are disclosed. The AmDH are variants of amino acid dehydrogenases. AmDH based on phenylalanine, leucine, and valine scaffolds are provided. The AmDH typically have one, two, three, four, or more amino acid alterations relative to the scaffold. The alterations to the scaffold result in an enzyme that accepts the analogous ketone, such as methyl isobutyl ketone (MIBK), instead of the wild-type ?-keto acid. Chimeric AmDH are also disclosed. The chimeras are fusion proteins that include a substrate binding domain from a first AmDH and a cofactor binding domain from a second AmDH. In a preferred embodiment, one of the domains is from a PheDH-based AmDH and one of the domains is from a LeuDH-based AmDH.Type: GrantFiled: May 15, 2013Date of Patent: September 16, 2014Assignee: Georgia Tech Research CorporationInventors: Andreas Sebastain Bommarius, Michael Justin Abrahamson, Bettina Bommarius
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Publication number: 20130309734Abstract: Non-naturally occurring amine dehydrogenases (AmDH) and methods of use thereof the produce chiral amines are disclosed. The AmDH are variants of amino acid dehydrogenases. AmDH based on phenylalanine, leucine, and valine scaffolds are provided. The AmDH typically have one, two, three, four, or more amino acid alterations relative to the scaffold. The alterations to the scaffold result in an enzyme that accepts the analogous ketone, such as methyl isobutyl ketone (MIBK), instead of the wild-type ?-keto acid. Chimeric AmDH are also disclosed. The chimeras are fusion proteins that include a substrate binding domain from a first AmDH and a cofactor binding domain from a second AmDH. In a preferred embodiment, one of the domains is from a PheDH-based AmDH and one of the domains is from a LeuDH-based AmDH.Type: ApplicationFiled: May 15, 2013Publication date: November 21, 2013Applicant: Georgia Tech Research CorporationInventors: Andreas Sebastain Bommarius, Michael Justin Abrahamson, Bettina Bommarius
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Patent number: 8530213Abstract: The present disclosure relates generally to bacterial NADH oxidases and, more particularly, to novel NADH oxidases obtained from Lactobacillus plantarum, and derivatives thereof that demonstrate enzymatic activity for NADH, NADPH, or both NADH and NADPH. The compositions comprising an NADH oxidase obtained from L. plantarum or derivatives thereof include: isolated enzymes; recombinantly produced enzymes and derivatives thereof, as well as catalytically active portions thereof; nucleic acids encoding an NADH oxidase obtained from L. plantarum, derivatives thereof, and portions thereof. The methods of the present invention include isolation of NADH oxidases obtained from L. plantarum, derivatives thereof, and portions thereof, and methods for enzymatic reactions comprising NADH oxidase obtained from L. plantarum, including the production of enantiomer-enriched organic compounds.Type: GrantFiled: December 2, 2010Date of Patent: September 10, 2013Assignees: Georgia Tech Research Corporation, Emory UniversityInventors: Andreas Bommarius, Bettina Bommarius, Jun-Ichiro Hirano, Vaijayanthi Thangavel, Jonathan Taejoo Park
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Publication number: 20120003688Abstract: The present disclosure relates generally to bacterial NADH oxidases and, more particularly, to novel NADH oxidases obtained from Lactobacillus plantarum, and derivatives thereof that demonstrate enzymatic activity for NADH, NADPH, or both NADH and NADPH. The compositions comprising an NADH oxidase obtained from L. plantarum or derivatives thereof include: isolated enzymes; recombinantly produced enzymes and derivatives thereof, as well as catalytically active portions thereof; nucleic acids encoding an NADH oxidase obtained from L. plantarum, derivatives thereof, and portions thereof. The methods of the present invention include isolation of NADH oxidases obtained from L. plantarum, derivatives thereof, and portions thereof, and methods for enzymatic reactions comprising NADH oxidase obtained from L. plantarum, including the production of enantiomer-enriched organic compounds.Type: ApplicationFiled: December 2, 2010Publication date: January 5, 2012Applicant: Georgia Tech Research CorporationInventors: Andreas Bommarius, Bettina Bommarius, Jun-Ichiro Hirano, Vaijayanthi Thangavel, Jonathan Taejoo Park
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Publication number: 20100048480Abstract: The present application provides methods of producing antimicrobial peptides (AMPs) in a cell, for example by expression a fusion protein that includes small ubiquitin related modifier (SUMO) and an AMP in the cell. Also provided are nucleic acid and protein sequences of SUMO-AMP fusion proteins, and kits that include such molecules.Type: ApplicationFiled: November 20, 2007Publication date: February 25, 2010Inventors: Bettina Bommarius, Melanie Sherman, Daniel Kalman, Xiadong Cheng
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Patent number: 7435574Abstract: The present invention is directed to a new NADH oxidase from Lactobacillus, nucleic acids encoding the NADH oxidase, methods of producing the NADH oxidase, as well as there use in producing improved NADH oxidase enzymes and for producing chrial enantiomer-enriched organic compounds, such as alcohols and/or amino acids.Type: GrantFiled: August 31, 2004Date of Patent: October 14, 2008Assignee: Degussa AGInventors: Werner Hummel, Bettina Bommarius
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Publication number: 20060240535Abstract: Compositions and methods of using a carbonyl reductase from Lactococcus lactis (LLCR or LL-CR) are provided. One aspect provides an isolated polypeptide from Lactococcus lactis, wherein the polypeptide is an (R)-specific carbonyl reductase or a fragment thereof and reduces acetophenone or alph,beta-unsaturated carbonyl compound to form a reduced R enantiomeric product. In certain aspects, LLCR is stable in from about 1.0% to about 60% organic solvent or more than 10%, 20%, 30%, 40% or 50% organic solvent.Type: ApplicationFiled: November 10, 2005Publication date: October 26, 2006Applicant: GEORGIA TECH RESEARCH CORPORATIONInventors: Andreas Bommarius, Bettina Bommarius
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Patent number: 6987013Abstract: The present invention is directed to a new NADH oxidase from Lactobacillus, nucleic acids encoding the NADH oxidase, methods of producing the NADH oxidase, as well as there use in producing improved NADH oxidase enzymes and for producing chrial enantiomer-enriched organic compounds, such as alcohols and/or amino acids.Type: GrantFiled: August 16, 2002Date of Patent: January 17, 2006Assignee: Degussa AGInventors: Werner Hummel, Bettina Bommarius
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Patent number: 6884602Abstract: This invention involves a process for selecting and producing eukaryotic alkaline phosphatase in yeast. Yeast cells are subjected to multiple transformations using a vector comprising a first resistance marker gene and the alkaline phosphatase gene. Those strains that grow in media containing the first resistance marker are further transformed using a vector comprising a second selection marker gene and the alkaline phosphatase gene. Transformants that grow in media containing the second selection marker are selected for expressing the eukaryotic alkaline phosphatase.Type: GrantFiled: July 23, 2001Date of Patent: April 26, 2005Assignee: Roche Diagnostics Operations, Inc.Inventors: Rainer Mueller, Johann-Peter Thalhofer, Frank Geipel, Werner Hoelke, Stephan Glaser, Hellmut Eckstein, Thomas Kirschbaum, Bettina Bommarius
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Publication number: 20050064570Abstract: The present invention is directed to a new NADH oxidase from Lactobacillus, nucleic acids encoding the NADH oxidase, methods of producing the NADH oxidase, as well as there use in producing improved NADH oxidase enzymes and for producing chrial enantiomer-enriched organic compounds, such as alcohols and/or amino acids.Type: ApplicationFiled: August 31, 2004Publication date: March 24, 2005Applicant: DEGUSSA AGInventors: Werner Hummel, Bettina Bommarius
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Publication number: 20030100065Abstract: The present invention is directed to a new NADH oxidase from Lactobacillus, nucleic acids encoding the NADH oxidase, methods of producing the NADH oxidase, as well as there use in producing improved NADH oxidase enzymes and for producing chrial enantiomer-enriched organic compounds, such as alcohols and/or amino acids.Type: ApplicationFiled: August 16, 2002Publication date: May 29, 2003Applicant: DEGUSSA AGInventors: Werner Hummel, Bettina Bommarius
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Publication number: 20030096341Abstract: The invention concerns a process for the recombinant production and expression of eukaryotic alkaline phosphatase in yeast cells in which in particular a DNA coding for a eukaryotic highly active alkaline phosphatase having a specific activity above 3000 U/mg is used. The invention additionally concerns a process for inserting corresponding nucleic acid sequences into a vector for expression in methylotrophic yeast strains and it concerns appropriate vectors and host strains.Type: ApplicationFiled: July 23, 2001Publication date: May 22, 2003Inventors: Rainer Mueller, Johann-Peter Thalhofer, Frank Geipel, Werner Hoelke, Stephan Glaser, Hellmut Eckstein, Thomas Kirschbaum, Bettina Bommarius