Patents by Inventor Betty H. Robertson

Betty H. Robertson has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • DNA sequence encoding a cynomolgus monkey hepatitis A virus capsid protein
    Patent number: 5430135
    Abstract: The present invention relates, in general, to substantially pure preparations of the cynomolgus monkey hepatitis A viral isolates CY-145 and CY-55/JM-55; cDNAs of the genomic RNAs of cynomolgus monkey hepatitis A viral isolates CY-145 and CY-55/JM-55; a method of preventing hepatitis A in an animal; and vaccines comprising the cynomolgus monkey hepatitis A viral isolates CY-145 and CY-55/JM-55.
    Type: Grant
    Filed: July 6, 1993
    Date of Patent: July 4, 1995
    Assignee: The United States of America as represented by the Department of Health and Human Services
    Inventors: Omana V. Nainan, Harold S. Margolis, Betty H. Robertson, Margo A. Brinton, James W. Ebert
  • Rapid and sensitive test for detecting hepatitis A virus
    Patent number: 5290677
    Abstract: Provided are methods for detecting hepatitis A virus (HAV) and HAV genotypes I and III by capturing whole hepatitis A virus with antibodies specific to hepatitis A virus, generating a cDNA copy of the RNA by reverse transcription in the presence of a negative strand primer having the sequence defined by SEQ ID NO:1, amplifying the cDNA by polymerase chain reaction with both the negative strand primer and a labelled or unlabelled positive strand primer having the sequence defined by SEQ ID NO: 2, and detecting the amplified cDNA by hybridization with either or both of the probes defined by SEQ ID NO:3 and SEQ ID NO:4 or by detection of label, wherein the presence of detectable hybridization or amplification indicates the presence of hepatitis A virus. Also provided are DNA primers and probes from the amino-terminal portion of the VPI region of the HAV genome that selectively hybridize with HAV, HAV genotype I and HAV genotype III and can amplify the amino-terminal portion of the VP1 region of HAV.
    Type: Grant
    Filed: January 31, 1992
    Date of Patent: March 1, 1994
    Assignee: The United States of America as represented by the Department of Health and Human Services
    Inventors: Betty H. Robertson, Omana V. Nainan, Vicki K. Brown, Harold S. Margolis, Bhawna Khanna
  • Reproducible generation of high yields of hepatitis A virus by cell culture
    Patent number: 5268292
    Abstract: The invention is a process for cell propagation of hepatitis A virus. The process involves purifying a culture of hepatitis A virus particles. These hepatitis A virus particles are used for infecting cells being cultivated in a media. The hepatitis A particles suitable for use in this invention must have a cytopathic effect in the infected host cells that are selected for culturing the hepatitis A virus. After developing the cytopathic effect in the infected cells, the hepatitis A viruses produce degeneration and lysis in the infected cells and are released into the media. Isolation of the released hepatitis A virus then occurs. The preferred cells for use in this invention are FRhK4 (foetal rhesus monkey kidney) cells. The preferred virus particles for use in this invention are hepatitis A, strain HAS-15 particles have an antigen coded by a nucleotide sequence according to FIG. 1.
    Type: Grant
    Filed: September 6, 1991
    Date of Patent: December 7, 1993
    Inventors: Betty H. Robertson, Rhawna Khanna, Vicki Brown, Harold S. Margolis