Abstract: Provided herein is a highly pure carbohydrate composition, and a method of making a highly pure carbohydrate composition. The method includes passing an aqueous carbohydrate solution through an anion exchange chromatography column including a polyethyleneimine (PEI) chromatographic media to obtain a purified solution, and isolating a highly pure carbohydrate composition from the purified solution.

Abstract: Chromatographic media of porous media particles derivatized with allylamine or polyallylamine obtained directly or through intermolecular polymerization on the surface thereof and such media functionalized with further functionalization groups. Such media are particularly useful for separating biomolecules.

Abstract: A flow-through process for separating protein monomer from aggregates of that protein in a solution containing both protein monomer and aggregates of that protein, the process includes the steps of 1) contacting the solution at a pH of from 4 to 7 with a weak anion exchange media comprised of multiple primary, secondary and/or tertiary amine functionalization groups whereby the protein monomer flows through the media without binding thereto and the aggregates are retained on the media, and 2) collecting the flow-through as purified protein monomer.