Abstract: The present invention relates to a process for integrated production of ethanol and seaweed biofertilizer from fresh red seaweed, Kappaphycus alvarezii. Specifically, the present invention describes a process for the production of ethanol, as a byproduct, from Kappaphycus alvarezii. The process includes crushing the fresh weeds to release the sap, a proved biofertilizer, and recovering the residual carrageenan rich biomass, hydrolyzing the biomass using dilute acid at elevated temperature, neutralizing the hydrolysate by using inexpensive calcium hydroxide and removing the insoluble salts through filtration or centrifugation, desalting soluble salts from the hydrolysate by electrodialysis, enriching with nitrogen source, inoculating with yeast and fermenting it to form a fermented broth containing ethanol and separating ethanol from the fermented broth by distillation and using residual hydrolysate, generated CaSO4 and reject obtained from electrodialysis as manure.

Abstract: The present invention relates to a process for integrated production of ethanol and seaweed biofertilizer from fresh red seaweed, Kappaphycus alvarezii. Specifically, the present invention describes a process for the production of ethanol, as a byproduct, from Kappaphycus alvarezii. The process includes crushing the fresh weeds to release the sap, a proved biofertilizer, and recovering the residual carrageenan rich biomass, hydrolysing the biomass using dilute acid at elevated temperature, neutralizing the hydrolysate by using inexpensive calcium hydroxide and removing the insoluble salts through filtration or centrifugation, desalting soluble salts from the hydrolysate by electrodialysis, enriching with nitrogen source, inoculating with yeast and fermenting it to form a fermented broth containing ethanol and separating ethanol from the fermented broth by distillation and using residual hydrolysate, generated CaSO4 and reject obtained from electrodialysis as manure.

Abstract: The present invention relates to a solid nutrient media composition having alkaline pH, useful for isolating and identifying alkaliphilic microorganisms in pure form. The media composition consists of 5-15 g of carbon source, 2.5-10 g of peptone, 2.5-10 g of yeast extract, 0.5-1.5 g of dipotassium hydrogen phosphate; 0.1-0.5 g of magnesium sulphate heptahydrate, 30 ?l-4 ml of super saturated solution of sodium hydroxide, 5-20 g of potassium chloride and 10-30 g of ?-carrageenan in one liter of distilled water. The potassium salt in combination with ?-carrageenan in specific proportion has been found to be a suitable replacement of agar in solidifying bacteriological media especially, for isolation of extreme alkaliphiles. The present invention also provides a method of using the solid nutrient media composition having alkaline pH to study biodiversity of cultivable alkaliphilic bacteria.

Abstract: Halophilic marine gram negative bacteria, isolated from decayed algae, hydrolyzed ?-carrageenan. To maximize ?-carrageenase production in the cost effective manner, a novel medium was defined having minimum components and their optimum concentration by statistical optimization method. Hence there evolved a novel medium composition for enhanced ?-carrageenase production by a new halotolerant marine bacterium, Pseudomonas spp.

Abstract: The present invention relates to a solid nutrient media composition having alkaline pH, useful for isolating and identifying alkaliphilic microorganisms in pure form. Te media composition consists of 5-15 g of carbon source, 2.5-10 g of peptone, 2.5-10 g of yeast extract, 0.5-1.5 g of dipotassium hydrogen phosphate; 0.1-0.5 g of magnesium sulphate heptahydrate, 30 ?l-4 ml of super saturated solution of sodium hydroxide, 5-20 g of potassium chloride and 10-30 g of ?-carrageenan in one litre of distilled water. The potassium salt in combination with ?-carrageenan in specific proportion has been found to be a suitable replacement of agar in solidifying bacteriological media especially, for isolation of extreme alkaliphiles. The present invention also provides a method of using the solid nutrient media composition having alkaline pH to study biodiversity of cultivable alkaliphilic bacteria.

Abstract: Halophilic marine gram negative bacteria, isolated from decayed algae, hydrolyzed ?-carrageenan. To maximize ?-carrageenase production in the cost effective manner, a novel medium was defined having minimum components and their optimum concentration by statistical optimization method. Hence there evolved a novel medium composition for enhanced ?-carrageenase production by a new halotolerant marine bacterium, Pseudomonas spp.