Abstract: A method includes, by a computing system, receiving a querying image depicting a sampling area, processing the querying image using a single cluster detection model to identify one or more regions of the querying image depicting a cluster in the sampling area, processing the one or more regions using a cluster verification deep-learning model to determine whether each depicted cluster is a cell cluster, and determining that exactly one of the identified one or more regions depicts a cluster that is a cell cluster. The method further includes processing the region depicting the cell cluster using a morphology deep-learning model to determine that there is only one cell in the cell cluster and to determine that die morphology of the cell is acceptable.

Abstract: Methods and system for real-time monitoring and control of an ultrafiltration/diafiltration (UF/DF) conditioned pool in a UF/DF recovery tank are disclosed. In various embodiments, a UF/DF pool is received at a recovery tank for conditioning or dilution by a buffer. Inline Raman measurements of the conditioned UF/DF pool may be performed and provided to a trained machine learning model as input. The machine learning model can then predict product quality attributes of the UF/DF conditioned pool, examples of said product quality attributes including protein concentration and osmolality of the conditioned UF/DF pool.

Abstract: A method includes, by a computing system, receiving a querying image depicting a sampling area, processing the querying image using a single cluster detection model to identify one or more regions of the querying image depicting a cluster in the sampling area, processing the one or more regions using a cluster verification deep-learning model to determine whether each depicted cluster is a cell cluster, and determining that exactly one of the identified one or more regions depicts a cluster that is a cell cluster. The method further includes processing the region depicting the cell cluster using a morphology deep-learning model to determine that there is only one cell in the cell cluster and to determine that die morphology of the cell is acceptable.