Abstract: The present disclosure is directed to novel methods for genetic immunization which utilize a eukaryote host with a non-eukaryotic expression system. Preferably the non-eukaryotic expression system is a bacteriophage RNA Polymerase, for example T7 RNA Polymerase, which is preferably expressed in specific tissue(s) of the host. Genetic immunization occurs when the eukaryote host is exposed to a polynucleotide construct encoding a specific immunogen operably linked to a promoter recognized by the non-eukaryotic expression system, which generates expression of the immunogen, thereby eliciting a humoral and/or cellular immune response in the host. The present disclosure is also directed to transgenic animals that express the non-eukaryotic expression system.