Abstract: Provided is a high-throughput coupled enzyme method of screening for a tryptophan-2,3-dioxygenase (TDO) inhibitor compound and/or an indoleamine-2,3-dioxygenase (IDO) inhibitor compound, which method comprises: (a) reacting tryptophan with isolated IDO and/or isolated TDO in the presence of a test compound to form N-formylkynurenine; (b) reacting N-formylkynurenine from step (a) with isolated kynurenine formamidase to form kynurenine; and (c) detecting the kynurenine produced in step (b) and determining whether the test compound is a TDO and/or an IDO inhibitor compound or not from the presence or absence or quantity of the detected kynurenine, wherein step (a) is conducted in the presence of a reducing system suitable for converting IDO and/or TDO from the Fe3+ to the Fe2+ state, and which does not prevent the formation of kynurenine in step (c).

Abstract: Provided is a high-throughput coupled enzyme method of screening for a tryptophan-2,3-dioxygenase (TDO) inhibitor compound and/or an indoleamine-2,3-dioxygenase (IDO) inhibitor compound, which method comprises: (a) reacting tryptophan with isolated IDO and/or isolated TDO in the presence of a test compound to form N-formylkynurenine; (b) reacting N-formylkynurenine from step (a) with isolated kynurenine formamidase to form kynurenine; and (c) detecting the kynurenine produced in step (b) and determining whether the test compound is a TDO and/or an IDO inhibitor compound or not from the presence or absence or quantity of the detected kynurenine, wherein step (a) is conducted in the presence of a reducing system suitable for converting IDO and/or TDO from the Fe3+ to the Fe2+ state, and which does not prevent the formation of kynurenine in step (c).

Abstract: A method of cementing a wellbore in a subterranean formation comprising formulating a non-Portland base cement composition that may be suitable for long-term zonal isolation, preparing the non-Portland base cement composition, determining a density of the non-Portland base cement composition and adjusting the density as needed to within an optimized density range to form a first optimized cement composition, determining the percentage of tensile strength relative to compressive strength of the cement composition, and placing the optimized cement composition in the wellbore.

Abstract: An advanced warning system for the physically challenged, which includes a first device disposed in the vicinity of a hazard for the physically challenged and including a transmitter and an antenna disposed for transmitting radio signals warning of the presence of the hazard. The system further includes a second device located with or on the physically challenged and including a receiver adapted for receiving the warning signal from the first device and including structure for indicating to the physically challenged user that a hazard is nearby. The system may be configured to include a plurality of the first devices distributed throughout an area having an equal plurality of potential hazards. In such configuration, the system may include a central radio station adapted for sending control update signals to each of the first devices.