Abstract: The present invention provides a quantum dot composite brightness enhancement film and a method for manufacturing same, relating to the field of optical thin films. The quantum dot composite brightness enhancement film includes a quantum dot film layer formed by a back coating layer, a substrate layer, a first barrier layer, a quantum dot layer, and a second barrier layer which are sequentially attached, a composite brightness enhancement film layer formed by a diffusion layer, a core layer, and a prism layer which are sequentially attached, and an Optically Clear Adhesive (OCA) layer connecting the second barrier layer and the diffusion layer.

Abstract: The present invention provides a quantum dot composite brightness enhancement film and a method for manufacturing same, relating to the field of optical thin films. The quantum dot composite brightness enhancement film includes a quantum dot film layer formed by a back coating layer, a substrate layer, a first barrier layer, a quantum dot layer, and a second barrier layer which are sequentially attached, a composite brightness enhancement film layer formed by a diffusion layer, a core layer, and a prism layer which are sequentially attached, and an Optically Clear Adhesive (OCA) layer connecting the second barrier layer and the diffusion layer.

Abstract: It relates to a method for preparation of four kinds of 2?, 3?-cNMPs (2?, 3?-cAMP, 2?, 3?-cGMP, 2?, 3?-cCMP and 2?, 3?-cUMP), comprising steps of: (1) extract genomic DNA and amplify gene If3; (2) ligate If3 gene to expression plasmid to construct a recombinant vector, and transfer the recombinant vector to E. coli to obtain a recombinant strain. Cultivate the recombinant strain and collect the fermentation broth; (3) collect the cells form the fermentation broth and disrupt the cells, and then purify the recombinant protein IF3 from the cell extract by Ni2+-nitrilotriacetic acid resin. Incubate the recombinant protein IF3 solution at 0° C. for 3 days to release 2?, 3?-cNMPs from IF3, and centrifuge the solution; (4) Ultrafiltrate the supernatant to remove proteins, and prepare four kinds of 2?, 3?-cNMPs by high-performance liquid chromatographic (HPLC) on a C18 reversed-phase column.

Abstract: It relates to a LfliZ gene and its application. The sequence of gene LfliZ is shown in SEQ ID NO.1. This invention also relates to the recombinant protein LfliZ encoded by LfliZ gene and its application in preparation of 2?, 3?-cNMPs. The recombinant LfliZ protein encoded by gene LfliZ can bind four kinds of 2?, 3?-cNMPs during its expression in Escherichia coli. Four kinds of 2?, 3?-cNMPs can be prepared simultaneously from the recombinant E. coli by extracting the recombinant protein LfliZ. The yield of 2?, 3?-cNMPs reaches 2.5 mg/L fermentation broth by the method in the present invention, indicating that this method has a good application potential.

Abstract: It relates to a method for preparation of four kinds of 2?, 3?-cNMPs (2?, 3?-cAMP, 2?, 3?-cGMP, 2?, 3?-cCMP and 2?, 3?-cUMP), comprising steps of: (1) extract genomic DNA and amplify gene If3; (2) ligate If3 gene to expression plasmid to construct a recombinant vector, and transfer the recombinant vector to E. coli to obtain a recombinant strain. Cultivate the recombinant strain and collect the fermentation broth; (3) collect the cells form the fermentation broth and disrupt the cells, and then purify the recombinant protein IF3 from the cell extract by Ni2+-nitrilotriacetic acid resin. Incubate the recombinant protein IF3 solution at 0° C. for 3 days to release 2?, 3?-cNMPs from IF3, and centrifuge the solution; (4) Ultrafiltrate the supernatant to remove proteins, and prepare four kinds of 2?, 3?-cNMPs by high-performance liquid chromatographic (HPLC) on a C18 reversed-phase column.

Abstract: It relates to a LfliZ gene and its application. The sequence of gene LfliZ is shown in SEQ ID NO.1. This invention also relates to the recombinant protein LfliZ encoded by LfliZ gene and its application in preparation of 2?,3?-cNMPs. The recombinant LfliZ protein encoded by gene LfliZ can bind four kinds of 2?,3?-cNMPs during its expression in Escherichia coli. Four kinds of 2?,3?-cNMPs can be prepared simultaneously from the recombinant E. coli by extracting the recombinant protein LfliZ. The yield of 2?,3?-cNMPs reaches 2.5 mg/L fermentation broth by the method in the present invention, indicating that this method has a good application potential.