Abstract: The present invention discloses a yeast strain with high yield of glutathione, and belongs to the technical field of microorganisms. Saccharomyces cerevisiae tlj2016 provided in the present invention has been deposited in China General Microbiological Culture Collection Center of Microbe Preservation Management Committee, with a preservation number of CGMCC No. 12789. The strain can tolerate glucose with tolerance capability reaching 300 g/L, and has tolerance capability against L-cysteine that is far higher than that of the starting strain, contributing to the production of glutathione at conditions of high concentrations of glucose, with a final concentration of GSH produced through fermentation in a 5-L fermentation tank reaching 3308 mg/L.

Abstract: The present invention discloses a yeast strain with high yield of glutathione, and belongs to the technical field of microorganisms. Saccharomyces cerevisiae tlj2016 provided in the present invention has been deposited in China General Microbiological Culture Collection Center of Microbe Preservation Management Committee, with a preservation number of CGMCC No. 12789. The strain can tolerate glucose with tolerance capability reaching 300 g/L, and has tolerance capability against L-cysteine that is far higher than that of the starting strain, contributing to the production of glutathione at conditions of high concentrations of glucose, with a final concentration of GSH produced through fermentation in a 5-L fermentation tank reaching 3308 mg/L.