Abstract: The present disclosure discloses a vortex-pair beam based optical tweezer system, including a laser device (1), a collimating beam expanding system, a spatial light modulator (6), a confocal beam shrinking system, a sample table (12), and an observation unit arranged according to a light path. The spatial light modulator (6) continuously loads different vortex-pair beam phase diagrams in real time, and manipulates and rotates a particle in real time by using a single vortex-pair beam. The optical tweezer system can realize precise regulation, control, and positioning of two spherical particles at any positions in a plane, and any controllable rotation operation of a rod-shaped particle in the plane, which makes application objects of the optical tweezer system richer, and effectively solves the problem that the rod-shaped particle is difficult to be controlled by the existing optical tweezer system.