Abstract: The present disclosure provides a virus composition. The efficacy of a variety of virus compositions loaded with different functional genes was detected, and several virus compositions with excellent antitumor effect were confirmed.

Abstract: Provided are a method for preparing an mRNA-LNP and use thereof, on which a technology platform of an uncapped-linear mRNA with unmodified uridine is based. The provided linear mRNA is composed of a 5? UTR including the IRES, a coding region and a poly A region has a simple structure and high stability and efficiency on its expression in vivo and in vitro. The uncapped-linear mRNA with unmodified uridine is then encapluslated by a new lipid nanoparticle (LNP-1) to form an mRNA-LNP.

Abstract: The present disclosure provides a virus composition. The efficacy of a variety of virus compositions loaded with different functional genes was detected, and several virus compositions with excellent antitumor effect were confirmed.

Abstract: The present disclosure provides a bispecific single-chain antibody, recombinant oncolytic virus for expressing same and virus composition. The antibody named BiTEs-PD-L1 is a bispecific antibody capable of simultaneously binding CD3 and PD-L1 on the surfaces of tumor cells, and it can effectively activate T cells and guide T cells to kill tumor cells. The oncolytic virus oHSV2-BiTEs-PD-L1 is further developed by utilizing the BiTEs-PD-L1, it can reduce frequency and dosage of the administration. The present disclosure also confirmed several virus compositions with excellent antitumor effect.

Abstract: The present disclosure provides a bispecific single-chain antibody, recombinant oncolytic virus for expressing same and virus composition. The antibody named BiTEs-PD-L1 is a bispecific antibody capable of simultaneously binding CD3 and PD-L1 on the surfaces of tumor cells, and it can effectively activate T cells and guide T cells to kill tumor cells. The oncolytic virus oHSV2-BiTEs-PD-L1 is further developed by utilizing the BiTEs-PD-L1, it can reduce frequency and dosage of the administration. The present disclosure also confirmed several virus compositions with excellent antitumor effect.

Abstract: The present disclosure a method for activating immunocytes in vitro by VAK technique. Autologous immunocytes of the cancer patient can be activated in vitro by the VAK technique, and by re-infusing these activated immunocytes into the patient's body, a good anti-tumor effect can be achieved, and because these immunocytes are autologous immunocytes, there is no rejection, being safe and reliable.

Abstract: The present invention discloses a use of recombinant oncolytic type II herpes simplex virus in preparing medicines against lymphoma, esophageal cancer, breast cancer and pancreatic cancer. The recombinant oncolytic type II herpes simplex virus can specifically grow and reproduce in human tumor cells, without affecting the proliferation of normal cells, thus it can effectively kill cancer cells, presenting good effect. These new indications provide more evidences for drug development and utilization with the recombinant oncolytic type II herpes simplex virus.