Abstract: The present invention relates to a variety of genes abnormally expressed in heart tissue as well as to fragments of such genes. Assessment of the expression level of these genes may be used for testing the predisposition of mammals and preferably humans for a heart disease or for an acute state of such a disease. Preferred diseases in accordance with the invention are congestive heart failure, dilative cardiomyopathy, hypertrophic cardiomyopathy and ischemic cardiomyopathy. The present invention further relates to methods of identifying compounds capable of normalizing the expression level of the aforementioned genes and of further genes affected by the abnormal expression. The identified compounds may be used for formulating compositions, preferably pharmaceutical compositions for preventing or treating diseases. They may also be used as lead compounds for the development of medicaments having an improved efficiency, a longer half-life, a decreased toxicity etc.

Abstract: The invention relates to a novel method for analyzing the composition of an mRNA sample and analyzing differential gene expression using two differently labeled primers, and to the use of the method. In the method for analyzing an RNA sample, a) a first primer, which is, where appropriate, labeled with a first dye, is used to prepare the first strand of a complementary DNA sample or cDNA sample from an RNA sample, b) a second primer, which is preferably labeled with a second dye, is used to prepare the second strand of this cDNA sample, c) the first primer, which is labeled with a first dye, and the second primer, which is labeled with a second dye, are used to amplify the cDNA sample, and d) the composition of the amplified, labeled cDNA sample is analyzed.

Abstract: The invention relates to a novel method for analyzing the composition of an mRNA sample and analyzing differential gene expression using two differently labeled primers, and to the use of the method.

Abstract: The invention relates to a novel method for analyzing the composition of an mRNA sample and analyzing differential gene expression using two differently labeled primers, and to the use of the method. In the method for analyzing an RNA sample, a) a first primer, which is, where appropriate, labeled with a first dye, is used to prepare the first strand of a complementary DNA sample or cDNA sample from an RNA sample, b) a second primer, which is preferably labeled with a second dye, is used to prepare the second strand of this cDNA sample, c) the first primer, which is labeled with a first dye, and the second primer, which is labeled with a second dye, are used to amplify the cDNA sample, and d) the composition of the amplified, labeled cDNA sample is analyzed.