Abstract: A computer-implemented segmentation method is used to process an image representing a plurality of nuclei. The method is implemented in a computer having a processor and a physical memory. A set of instructions are provided to the processor the physical memory of the computer. The processor is configured by executing the set of instructions in the physical memory so as to automatically segment the image by: thresholding a grey-scale image to create a black and white image; identifying objects in the black and white image and removing objects failing to meet predetermined criteria; extracting objects; and applying an edge detector on the segmented image to identify the edges of the nuclei. Overlapping nuclei are split to improve results.

Abstract: A computer-implemented segmentation method is used to process an image representing a plurality of nuclei. The method is implemented in a computer having a processor and a physical memory. A set of instructions are provided to the processor the physical memory of the computer. The processor is configured by executing the set of instructions in the physical memory so as to automatically segment the image by: thresholding a grey-scale image to create a black and white image; identifying objects in the black and white image and removing objects failing to meet predetermined criteria; extracting objects; and applying an edge detector on the segmented image to identify the edges of the nuclei. Overlapping nuclei are split to improve results.

Abstract: Disclosed is a laser system that comprises a laser source adapted to emit a laser beam, wherein the laser source comprises a plurality of emitters for emitting respective beam contributions to the emitted laser beam; and first and second feedback means adapted to provide optical feedback to respective first and second parts of the emitted laser beam. The first feedback means is adapted to predominantly feed back a respective angular/directional contribution of each of said beam contributions into a corresponding one of the plurality of emitters from which said angular/directional contribution was emitted at a predetermined output angle, to cause each of the plurality of emitters to predominantly emit a beam contribution towards the second feedback means at an angle corresponding to the predetermined output angle.