Abstract: The present invention relates to a method of isolating covalently closed circular (ccc) DNA molecules from microbial cells containing the ccc DNA molecules, comprising the steps of: contacting the microbial cells with a lysing agent and moving the composition through a tube system with a flow having a Reynolds number of at least 3000 to obtain a lysing composition, incubating the lysing composition to obtain a lysate, contacting the lysate with a neutralizing solution to obtain a neutralized lysate, and further processing the neutralized lysate to obtain the ccc DNA molecules.

Abstract: A system for and method of mapping and monitoring posture disclosed. The system includes sensor units that independently collect posture data corresponding to body locations or body positions on subjects and generate posture signals. The posture signals are wirelessly transmitted to one or more computers that are used to process the posture signals and generate posture feedback of the subjects in real-time. The sensor units are preferably accelerometer sensor units. Sensor units are assigned or learn the body locations or body positions, classify body locations or body positions, map and monitor posture, assign posture or activities of subjects and transmit the posture signals to the one or more computers by low-power radio transmitters to provide posture feedback. The system collectively is adaptive for mapping and monitoring posture from body locations or body positions corresponding to spines, arms, legs, necks, heads, thighs, hips, feet and hands of the subjects.