Abstract: The present invention relates to a method and a system for the overall brightness of at least one object in a digital image. In particular, the method for determining the overall brightness of at least one object in a digital image, the at least one object being configured to receive molecules comprising genetic information, the method comprising the steps of: generating an area A comprising k sample points, the k points being distributed in the area A according to a predetermined distribution; aligning the center position of the area A to the center position of the at least one object in the digital image; extracting the intensity value at each of the k sample points; and calculating the central tendency of the extracted intensity values at the respective k sample points.

Abstract: System, method and computer program product for background compensation of a digital image containing at least one first object, the at least one first object having received at least one molecule comprising genetic information, and at least one second object, the at least one second object not having received a molecule comprising genetic information, the at least one molecule being configured to receive a fluorescent compound, the digital image being determined by an optical imaging system during emission of electromagnetic radiation by the fluorescent compound.

Abstract: The present invention relates to a method and a system for determining the positions of a plurality of objects in a digital image by discriminating true positive positions of the plurality of objects from false positive candidate positions of the plurality of objects. In particular, the invention relates to a method for determining the positions of a plurality of objects in a digital image by discriminating true positive positions of the plurality of objects from false positive candidate positions of the plurality of objects, the plurality of objects being configured to receive molecules comprising genetic information.

Abstract: System, method and computer program product for background compensation of a digital image containing at least one first object, the at least one first object having received at least one molecule comprising genetic information, and at least one second object, the at least one second object not having received a molecule comprising genetic information, the at least one molecule being configured to receive a fluorescent compound, the digital image being determined by an optical imaging system during emission of electromagnetic radiation by the fluorescent compound.

Abstract: The present invention relates to a method and a system for the overall brightness of at least one object in a digital image. In particular, the method for determining the overall brightness of at least one object in a digital image, the at least one object being configured to receive molecules comprising genetic information, the method comprising the steps of: generating an area A comprising k sample points, the k points being distributed in the area A according to a predetermined distribution; aligning the center position of the area A to the center position of the at least one object in the digital image; extracting the intensity value at each of the k sample points; and calculating the central tendency of the extracted intensity values at the respective k sample points.

Abstract: The present invention relates to a method and a system for determining the positions of a plurality of objects in a digital image by discriminating true positive positions of the plurality of objects from false positive candidate positions of the plurality of objects. In particular, the invention relates to a method for determining the positions of a plurality of objects in a digital image by discriminating true positive positions of the plurality of objects from false positive candidate positions of the plurality of objects, the plurality of objects being configured to receive molecules comprising genetic information.