Abstract: The invention pertains to a method for producing a shoot of a plant, wherein the shoot comprises one or more cells having a genetic modification. The method preferably comprises the steps of: i) providing a plant, ii) introducing in a cell of the plant a vector expressing at least one of a gRNA and a CRISPR-nuclease, iii) removing the shoot apical meristem of the plant, and iv) regenerating a shoot at a second location in the plant, wherein the shoot comprises one or more cells having a genetic modification. The shoot apical meristem of the plant is preferably removed by decapitating the plant. Similarly, the shoot is preferably regenerated by a second decapitation of the plant at a second location. The invention further related to a plant having a shoot comprising one or more cells having a genetic modification, wherein the plant is obtainable by the method of the invention.

Abstract: The invention concerns the targeted genomic modification of a plant cell, preferably a meristem cell. More in particular, the invention pertains to a vector expressing a coding RNA, wherein the coding RNA comprises a sequence encoding a CRISPR-nuclease and a mobile element, wherein the mobile element enables intercellular translocation of the coding RNA, preferably intercellular translocation to a meristem cell. The invention further concerns an editing RNA comprising the coding RNA and further comprising a guide RNA.

Abstract: Genetic modification of plants is hampered by the limited capacity of plant cells to regenerate. The current invention solves this problem by introducing or increasing the expression of a histidine kinase in a plant cell. Preferred histidine kinases are at least one of CHK2, CHK3 and CHK4. The invention therefore concerns a method for improving a cytokinin-induced regeneration capacity of a plant cell, wherein the method comprises a step of increasing or introducing the expression of a histidine kinase in the plant cell. The invention further pertains to a method for regenerating a plant, wherein the method comprises a step of introducing or increasing the expression of a histidine kinase and to a plant obtainable from such method. Moreover, the method concerns the use of at least one of CHK2, CHK3 and CHK4 for improving a cytokinin-induced regeneration capacity of a plant.