Abstract: A cold rolled steel flat product for packaging made of a low carbon steel having a thickness of less than 0.49 mm and a method of making. The steel flat product has a martensite-free microstructure and represents a standard grade for packaging with tensile strengths from 300 to 550 MPa, which can be produced from a cold-rolled steel sheet with a carbon content from 0.01% to 0.1% by weight by inductive annealing of the steel sheet and subsequent water cooling for quenching the recrystallization-annealed steel sheet. To achieve flatness of 5 I-units or less, the induction annealed steel sheet is first primarily cooled in the manufacturing process to a take-off temperature at a rate of less than 1000 K/s, with the take-off temperature being below the transformation temperature of 723° C., and thereafter a secondary cooling by water cooling with a water temperature of less than 80° C. at a rate of more than 1000 K/s.

Abstract: The invention relates to a nucleotide sequence coding for the mannitol 2-dehydrogenase and a method for producing D-mannitol. Previously known biocatalytic methods for producing D-mannitol yield only small production rates due to the small number of specific activities of mannitol 2-dehydrogenases used for transforming D-fructose into D-mannitol. D-mannitol production can be improved by supplying a nucleotide sequence which codes for a mannitol 2-dehydrogenase having a higher specific activity. Mannitol production can be increased in a particular manner by creating a regeneration system for reduction equivalents by introducing and/or strengthening a formiate dehydrogenase in a microorganism.