Abstract: Disclosed herein are systems for receiving a biological sample for analysis and methods for preparing and/or analyzing a biological sample. In various embodiments, a system includes a first layer comprising a first region configured to receive a sample, a gasket disposed on the first layer such that the gasket surrounds the first region, and a housing layer. The first layer is disposed between the gasket and the housing layer. The system further includes a clamp configured to apply a force on the gasket to form a liquid tight seal against the first layer.

Abstract: The present disclosure relates to methods for preparing biological samples for in situ analysis of one or more analytes wherein the biological sample has been previously affixed to a substrate, which in some cases may not be compatible with in situ analysis, for example, due to the absence of positional markers and/or fiducial markers and/or a region suitable for in situ signal detection on the substrate. In some aspects, a hydrophobic adapter having positional markers and/or fiducial markers is applied to the substrate to which a biological sample has been affixed, thus enabling in situ sample processing and analysis of the biological sample. The hydrophobic adhesive label or adapter can be used for automated microscope alignment and sample preparation.

Abstract: The present disclosure in some aspects relates to methods and compositions for accurately detecting and quantifying multiple analytes present in a biological sample. In some aspects, the methods and compositions provided herein address one or more issues associated with the stability and/or size of nucleic acid structures such as rolling circle amplification products in the biological sample.

Abstract: An asynchronous database API allows threads to call a database and continue to execute without spawning a thread to wait for the database and without polling the database. The API immediately returns to a caller without waiting for a database operation to complete or for any other external event. A call to an asynchronous database API can invoke an initialization method. The initialization method may validate the input parameters, build them into a message for a database operation, send the message to the database server, and return signal information to the client process thread. Signal information provides a client thread with a way to recognize a signal indicating the return of results from a database. Upon completion of a database operation, a thread can call a finalization method to perform any final step to process the database results and pass the formatted results to the thread.

Abstract: A database API is presented that can expose data of non-native data-types, i.e., types that are unknown at API design-time. A broad type-system is used that is understood by both a server and a client. The API can dynamically discover, at run-time, data types that were defined using this broad type-system. The API can retrieve metadata that references a non-native data type in a client type library. A retrieved instance of the non-native data type, in serialized form, may be deserialized using the metadata.

Abstract: A database API is presented that can expose data of non-native data-types, i.e., types that are unknown at API design-time. A broad type-system is used that is understood by both a server and a client. The API can dynamically discover, at run-time, data types that were defined using this broad type-system. The API can retrieve metadata that references a non-native data type in a client type library. A retrieved instance of the non-native data type, in serialized form, may be deserialized using the metadata.

Abstract: An asynchronous database API allows threads to call a database and continue to execute without spawning a thread to wait for the database and without polling the database. The API immediately returns to a caller without waiting for a database operation to complete or for any other external event. A call to an asynchronous database API can invoke an initialization method. The initialization method may validate the input parameters, build them into a message for a database operation, send the message to the database server, and return signal information to the client process thread. Signal information provides a client thread with a way to recognize a signal indicating the return of results from a database. Upon completion of a database operation, a thread can call a finalization method to perform any final step to process the database results and pass the formatted results to the thread.