Abstract: The present disclosure provides inhibitory peptides of mitochondrial fission protein 1 (Fis1), polynucleotides and vectors encoding the peptides, and methods of using the peptides to treat diseases, including arterial diseases and vascular dysfunction associated with type 2 diabetes.

Abstract: The present disclosure provides soluble mutant PD-L1 peptides, polynucleotides and vectors encoding the peptides, and methods of using the peptides to elicit differentiation of regulatory T cells.

Abstract: The present disclosure provides a method of treating mitochondrial and age-related diseases and disorders with exercised blood products. The blood products include circulating factors whose production or secretion into the blood is stimulated by exercise.

Abstract: A filter for an inflator includes a rolled sheet centered on an axis or three dimensionally printed as a whole and including a plurality of openings. A deflector is associated with each opening for directing inflation fluid in a non-radial direction relative to the axis.

Abstract: The present invention is based, at least in part, on the development of a mating-based yeast two-hybrid screen that allows simultaneous screening for mutations that disrupt yeast two-hybrid interactions between a protein and multiple interacting partners. By coupling PCR mutagenesis and homologous recombination/gapped plasmid repair with a mating-based assay, the present invention allows screening for unique mutations that disrupt interaction with one partner, but not others. It also allows identification of specific mutations that may lie at protein-protein interfaces common to two or more partners, without employing multiple rounds of screening. In addition to screening against multiple interacting partners, the present invention removes the need for a two-step selection because truncations, frameshifts, or any mutations that affect folding are eliminated as disruptions that affect all protein partners.

Abstract: A filter for an inflator includes a rolled sheet centered on an axis or three dimensionally printed as a whole and including a plurality of openings. A deflector is associated with each opening for directing inflation fluid in a non-radial direction relative to the axis.

Abstract: The present disclosure provides a method of identifying factors for the treatment of mitochondrial and age-related diseases and disorders. Identification of the factors can comprise, e.g., biochemical, genomic, transcriptomic, proteomic and metabolomic analyses. The factors include proteins (e.g., cytokines) whose production or secretion into the blood is stimulated by exercise. The factors can be developed into therapeutics for the treatment of mitochondrial and age-related diseases and disorders.

Abstract: The present invention is based, at least in part, on the development of a mating-based yeast two-hybrid screen that allows simultaneous screening for mutations that disrupt yeast two-hybrid interactions between a protein and multiple interacting partners. By coupling PCR mutagenesis and homologous recombination/gapped plasmid repair with a mating-based assay, the present invention allows screening for unique mutations that disrupt interaction with one partner, but not others. It also allows identification of specific mutations that may lie at protein-protein interfaces common to two or more partners, without employing multiple rounds of screening. In addition to screening against multiple interacting partners, the present invention removes the need for a two-step selection because truncations, frameshifts, or any mutations that affect folding are eliminated as disruptions that affect all protein partners.

Abstract: Exemplary embodiments provide transportation management systems, devices and methods that allow display and management of transportation resources and operations in an integrated manner. Exemplary embodiments may provide, for example, a single dynamic and interactive interface for executing inbound transportation operations (procurement or pickup), outbound transportation operations (delivery or shipment), and operations in which multiple transportation resources are matched to one another.

Abstract: The present invention is based, at least in part, on the development of a mating-based yeast two-hybrid screen that allows simultaneous screening for mutations that disrupt yeast two-hybrid interactions between a protein and multiple interacting partners. By coupling PCR mutagenesis and homologous recombination/gapped plasmid repair with a mating-based assay, the present invention allows screening for unique mutations that disrupt interaction with one partner, but not others. It also allows identification of specific mutations that may lie at protein-protein interfaces common to two or more partners, without employing multiple rounds of screening. In addition to screening against multiple interacting partners, the present invention removes the need for a two-step selection because truncations, frameshifts, or any mutations that affect folding are eliminated as disruptions that affect all protein partners.

Abstract: Exemplary embodiments provide transportation management systems, devices and methods that allow display and management of transportation resources and operations in an integrated manner. Exemplary embodiments may provide, for example, a single dynamic and interactive interface for executing inbound transportation operations (procurement or pickup), outbound transportation operations (delivery or shipment), and operations in which multiple transportation resources are matched to one another.

Abstract: The present invention is based, at least in part, on the development of a mating-based yeast two-hybrid screen that allows simultaneous screening for mutations that disrupt yeast two-hybrid interactions between a protein and multiple interacting partners. By coupling PCR mutagenesis and homologous recombination/gapped plasmid repair with a mating-based assay, the present invention allows screening for unique mutations that disrupt interaction with one partner, but not others. It also allows identification of specific mutations that may lie at protein-protein interfaces common to two or more partners, without employing multiple rounds of screening. In addition to screening against multiple interacting partners, the present invention removes the need for a two-step selection because truncations, frameshifts, or any mutations that affect folding are eliminated as disruptions that affect all protein partners.