Patents by Inventor Bo-Chin Lei

Bo-Chin Lei has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • DEVELOPMENT OF THE SOLUBLE RECOMBINANT CRM197 PRODUCTION BY E. COLI
    Publication number: 20150184215
    Abstract: A method for recombinant production of a CRM197 protein includes culturing a recombinant Escherichia coli cell to produce said CRM197 protein, and isolating said CRM197 protein. The recombinant Escherichia coli cell includes an expression vector, which contains a nucleic acid molecule that encodes a fusion protein that includes an E. coli periplasmic signal peptide at N terminal and the CRM197 at C terminal. The CRM197 is encoded by a polynucleotide having the sequence of SEQ ID NO: 1. The E. coli periplasmic signal peptide comprise a pelB leader sequence. The nucleic acid molecule comprises the sequence of SEQ ID NO:2. The Escherichia coli cell is BL21(DE3)pLysS.
    Type: Application
    Filed: December 29, 2014
    Publication date: July 2, 2015
    Inventors: Yu-Shen Hsu, Show-Shan Sheu, Bo-Chin Lei, Tsan-Hui Wu