Abstract: The present invention relates to expression vectors and methods for enhancing soluble expression and secretion of an insoluble heterologous protein, particularly a bulky folded active heterologous protein which has one or more transmembrane-like domains or intramolecular disulfide bonds by linking a leader peptide with acidic or basic pI and high hydrophilicity thereto; by substituting one or more amino acids within N-terminal of the heterologous protein with ones having acidic or neutral pI and high hydrophilicity; or reducing elevating ?GRNA value of a polynucleotide encoding the leader peptide having basic pI value and high hydrophilicity. The expression vector and the method may be used to produce of heterologous protein and to transduce of therapeutic proteins in a patient by preventing formation of insoluble inclusion body and by enhancing secretional efficiency of the heterologous protein into the periplasm or outside cell.

Abstract: The present invention relates to an isolated Bacillus sp. strain having probiotic activity. More particularly, the present invention relates to an isolated Bacillus sp. 2-4 (KCCM11107P) strain having probiotic activity. Since the strain of the present invention functions as probiotics by having an antibacterial activity against various fish pathogenic bacteria, as well as secreting helpful enzymes such as protease, amylase, cellulose, and lipase to help the digestion and absorption of the feed, the strain of the present invention can be helpfully used as a feed additive for fish and crustaceans.

Abstract: The present invention relates to expression vectors and methods for enhancing soluble expression and secretion of an insoluble heterologous protein, particularly a bulky folded active heterologous protein which has one or more transmembrane-like domains or intramolecular disulfide bonds by linking a leader peptide with acidic or basic pl and high hydrophilicity thereto; by substituting one or more amino acids within N-terminal of the heterologous protein with ones having acidic or neutral pl and high hydrophilicity; or reducing elevating ?GRNA value of a polynucleotide encoding the leader peptide having basic pl value and high hydrophilicity. The expression vector and the method may be used to produce of heterologous protein and to transduce of therapeutic proteins in a patient by preventing formation of insoluble inclusion body and by enhancing secretional efficiency of the heterologous protein into the periplasm or outside cell.

Abstract: The present invention relates to a novel Bacillus sp. strain having probiotic activity. More particularly, the present disclosure relates to a novel Bacillus sp. 2-4 (KCCM11107P) strain having probiotic activity. Since the strain of the present disclosure functions as probiotics by having an antibacterial activity against various fish pathogenic bacteria, as well as secreting helpful enzymes such as protease, amylase, cellulose, and lipase to help the digestion and absorption of the feed, the strain of the present disclosure can be helpfully used as a feed additive for fish and crustaceans.

Abstract: The present invention relates to expression vectors and methods for enhancing soluble expression and secretion of a heterologous protein, particularly a bulky folded active heterologous protein which has one or more transmembrane-like domains or intramolecular disulfide bonds by linking a leader peptide with acidic or basic pI and high hydrophilicity thereto; by substituting one or more amino acids within N-terminal of the heterologous protein with ones having acidic or neutral pI and high hydrophilicity; or reducing elevating GRNA value of a polynucleotide encoding the leader peptide having basic pI value and high hydrophilicity. The expression vector and the method may be used to produce of heterologous protein and to transduce of therapeutic proteins in a patient by preventing formation of insoluble inclusion body and by enhancing secretional efficiency of the heterologous protein into the periplasm or outside cell.

Abstract: The present invention relates to a method for improving secretion efficiency of a recombinant foreign protein using a polypeptide fragment containing N-region of a signal sequence (directional signal) or variants thereof with the controlled pI value and/or a secretional enhancer composed of a hydrophilic polypeptide with the controlled pI value. The method of the present invention can be not only useful for the production of a recombinant foreign protein by preventing precipitation of an insoluble precipitate and by increasing extracellular or extra-periplasmic secretion efficiency of a recombinant protein but also useful for the transduction of an effective therapeutic protein by increasing membrane permeability using a strong secretional enhancer.

Abstract: The present invention is drawn to a method for enhancing secretional efficiency of a heterologous protein using a secretional enhancer consisting of a modified signal sequence which comprises the N-region of a signal sequence and/or a hydrophobic fragment of the said signal sequence comprising the said N-region and/or the hydrophilic polypeptide. The method of the present invention can be used not only for production of recombinant heterologous proteins by inhibiting insoluble precipitation and enhancing secretional efficiency of the recombinant protein into the periplasm or the extracellular fluid and but also for transduction of therapeutic proteins by enhancing membrane-permeability of the recombinant protein using a strong secretional enhancer.