Patents by Inventor Bong Hyun Sung
Bong Hyun Sung has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11365388Abstract: Provided is a method of producing a novel microorganism having a minimal genome. In particular, provided are a method of producing a novel microorganism having a smaller genome size than a wild-type, the method including the step of culturing the microorganism in a medium containing LB broth with stepwise decreasing concentrations of the LB broth; and a novel microorganism having a smaller genome size than a wild-type, produced by the above method. Despite the small genome size, the novel microorganism of the present disclosure may have a high growth rate, pyruvate production capacity, and protein translation efficiency thereby being usefully applied to industry such as production of recombinant proteins or pyruvate.Type: GrantFiled: January 24, 2020Date of Patent: June 21, 2022Assignee: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGYInventors: Sun-Chang Kim, Byung-Kwan Cho, Donghui Choe, Bong Hyun Sung, Jun Hyoung Lee
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Publication number: 20200239831Abstract: Provided is a method of producing a novel microorganism having a minimal genome. In particular, provided are a method of producing a novel microorganism having a smaller genome size than a wild-type, the method including the step of culturing the microorganism in a medium containing LB broth with stepwise decreasing concentrations of the LB broth; and a novel microorganism having a smaller genome size than a wild-type, produced by the above method. Despite the small genome size, the novel microorganism of the present disclosure may have a high growth rate, pyruvate production capacity, and protein translation efficiency thereby being usefully applied to industry such as production of recombinant proteins or pyruvate.Type: ApplicationFiled: January 24, 2020Publication date: July 30, 2020Inventors: Sun-Chang Kim, Byung-Kwan Cho, Donghui Choe, Bong Hyun Sung, Jun Hyoung Lee
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Patent number: 10443078Abstract: The present invention relates to: a novel Pichia kudriavzevii microorganism NG7 showing heat resistance and acid resistance; a composition, for producing organic acid or alcohol, which comprises the microorganism and a culture of the same; and a method, for producing an organic acid or alcohol, which comprises culturing the microorganism.Type: GrantFiled: June 22, 2015Date of Patent: October 15, 2019Assignee: Korea Research Institute of Bioscience and BiotechnologyInventors: Jung Hoon Sohn, Hyun Joo Park, Sun Hee Lee, Jung Hoon Bae, Bong Hyun Sung
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Patent number: 10406204Abstract: The present invention provides an antimicrobial peptide polymer comprising at least one monomer which is digested by pepsin, a multimeric antimicrobial peptide complex comprising the polymer and a cell surface anchoring motif linked to the polymer, an antimicrobial microorganism displaying the multimeric antimicrobial peptide complex, an antimicrobial composition comprising the same, a method of treating an infectious disease caused by bacteria, yeast or fungi by administering the antimicrobial composition, and a method for producing the antimicrobial microorganism. According to the invention, living microorganisms displaying an antimicrobial peptide on the cell surface thereof may be administered in vivo without having to lyse the microbial cell and isolate and purify the antimicrobial peptide, so that the antimicrobial peptide exhibits antimicrobial activity. Thus, the antimicrobial peptide may be produced at significantly reduced costs so that it may have widespread use.Type: GrantFiled: September 4, 2015Date of Patent: September 10, 2019Assignees: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY, INTELLIGENT SYNTHETIC BIOLOGY CENTERInventors: Sun-Chang Kim, Ju Ri Shin, Ki Jung Lim, Da Jung Kim, Young Woong Lee, Su A Jang, Bong Hyun Sung
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Publication number: 20170349920Abstract: The present invention relates to: a novel Pichia kudriavzevii microorganism NG7 showing heat resistance and acid resistance; a composition, for producing organic acid or alcohol, which comprises the microorganism and a culture of the same; and a method, for producing an organic acid or alcohol, which comprises culturing the microorganism.Type: ApplicationFiled: June 22, 2015Publication date: December 7, 2017Applicant: Korea Research Institute of Bioscience and BiotechnologyInventors: Jung Hoon Sohn, Hyun Joo Park, Sun Hee Lee, Jung Hoon Bae, Bong Hyun Sung
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Patent number: 9719112Abstract: The present invention relates to beta-glucosidase that is mutated to have enhanced activity, and a method for producing bioethanol using the same. More particularly, the present invention relates to a polynucleotide encoding beta-glucosidase that is mutated to have enhanced activity, beta-glucosidase expressed from the polynucleotide, an expression vector including the polynucleotide, a transformant that is transformed with the expression vector, a method for producing the mutated beta-glucosidase using the transformant, and a method for producing bioethanol using the transformant. The mutant beta-glucosidase of the present invention increases production of glucose much more than the conventional beta-glucosidase, and thus it can be widely used for economic production of bioethanol.Type: GrantFiled: April 13, 2012Date of Patent: August 1, 2017Assignee: KOREA RESEARCH INSITUTE OF BIOSCIENCE AND BIOTECHNOLOGYInventors: Jung Hoon Sohn, Hyun Jin Kim, Chul Ho Kim, Ji Hun Seomoon, Bong Hyun Sung, Kwang Mook Lim, Mi Jin Kim, Jong Hyun Kim
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Patent number: 9527886Abstract: Disclosed are an amphipathic peptide-lipase conjugate with enhanced lipase activity, a polynucleotide coding for the conjugate, an expression vector carrying the polynucleotide, a transformant anchoring the expression vector therein, a method for preparing the conjugate, a lipolysis method using the conjugate, and a method for producing biodiesel using the lipase.Type: GrantFiled: August 10, 2012Date of Patent: December 27, 2016Assignees: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY, INTELLIGENT SYNTHETIC BIOLOGY CENTERInventors: Sun-Chang Kim, Bong Hyun Sung, Kyung Seok Yang, Jun Hyoung Lee, Ki Jung Lim, Myung Keun Park
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Publication number: 20150366992Abstract: The present invention provides an antimicrobial peptide polymer comprising at least one monomer which is digested by pepsin, a multimeric antimicrobial peptide complex comprising the polymer and a cell surface anchoring motif linked to the polymer, an antimicrobial microorganism displaying the multimeric antimicrobial peptide complex, an antimicrobial composition comprising the same, a method of treating an infectious disease caused by bacteria, yeast or fungi by administering the antimicrobial composition, and a method for producing the antimicrobial microorganism. According to the invention, living microorganisms displaying an antimicrobial peptide on the cell surface thereof may be administered in vivo without having to lyse the microbial cell and isolate and purify the antimicrobial peptide, so that the antimicrobial peptide exhibits antimicrobial activity. Thus, the antimicrobial peptide may be produced at significantly reduced costs so that it may have widespread use.Type: ApplicationFiled: September 4, 2015Publication date: December 24, 2015Inventors: Sun-Chang Kim, Ju Ri Shin, Ki Jung Lim, Da Jung Kim, Young Woong Lee, Su A Jang, Bong Hyun Sung
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Publication number: 20150299733Abstract: The present invention relates to beta-glucosidase that is mutated to have enhanced activity, and a method for producing bioethanol using the same. More particularly, the present invention relates to a polynucleotide encoding beta-glucosidase that is mutated to have enhanced activity, beta-glucosidase expressed from the polynucleotide, an expression vector including the polynucleotide, a transformant that is transformed with the expression vector, a method for producing the mutated beta-glucosidase using the transformant, and a method for producing bioethanol using the transformant. The mutant beta-glucosidase of the present invention increases production of glucose much more than the conventional beta-glucosidase, and thus it can be widely used for economic production of bioethanol.Type: ApplicationFiled: April 13, 2012Publication date: October 22, 2015Applicant: KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGYInventors: Jung Hoon Sohn, Hyun Jin Kim, Chul Ho Kim, Ji Hun Seomoon, Bong Hyun Sung, Kwang Mook Lim, Mi Jin Kim, Jong Hyun Kim
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Patent number: 9067970Abstract: The present invention relates a prophylactic or therapeutic composition for cancer, and more particularly, to a prophylactic or therapeutic composition for cancer comprising a peptide which is represented by an amino acid sequence of the following Formula (I), a method for preventing or treating cancer comprising the step of administering the peptide to a subject, and use of the peptide in the preparation of the prophylactic or therapeutic composition for cancer. (I) APKAMX1LLX2X3L-LX4LQKKGI wherein X1, X2, X3 and X4 are each independently R or K.Type: GrantFiled: December 24, 2010Date of Patent: June 30, 2015Assignees: Korea Advanced Institute of Science and Technology, Intelligent Synthetic Biology CenterInventors: Sun Chang Kim, Su A Jang, Da Jung Kim, Bong Hyun Sung, Ki Jeong Lim, Ju Ri Shin, Young Woong Lee
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Patent number: 8871716Abstract: Disclosed are novel antimicrobial peptides which can promote the regeneration of skin cells, thus healing wounds. Pharmaceutical compositions comprising the peptides as active ingredients are also provided for wound healing and skin rejuvenation. The antimicrobial peptides exhibit inhibitory activity against antibiotic-resistant strains, and their antimicrobial activity is maintained without loss of structural stability even under a high salt condition. Also, being proven to promote the migration and regeneration of skin cells in mice as well as in vitro, the antimicrobial peptides may be widely used as an agent for regenerating skin cells. Further, they can find applications in various fields including the medical industry and the cosmetic industry. Hence, the novel antimicrobial peptides are anticipated to have considerable repercussions in the market for antibiotics, wound healing agents and cosmetics.Type: GrantFiled: April 27, 2011Date of Patent: October 28, 2014Assignees: Korea Advanced Institute of Science and Technology, Intelligent Synthetic Biology CenterInventors: Sun-Chang Kim, Da-Jung Kim, Su-A Jang, Bong Hyun Sung, Ki-Jung Lim, Ju-Ri Shin, Young Woong Lee
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Publication number: 20140162331Abstract: Disclosed are an amphipathic peptide-lipase conjugate with enhanced lipase activity, a polynucleotide coding for the conjugate, an expression vector carrying the polynucleotide, a transformant anchoring the expression vector therein, a method for preparing the conjugate, a lipolysis method using the conjugate, and a method for producing biodiesel using the lipase.Type: ApplicationFiled: August 10, 2012Publication date: June 12, 2014Inventors: Sun-Chang Kim, Bong Hyun Sung, Kyung Seok Yang, Jun Hyoung Lee, Ki Jung Lim, Myung Keun Park
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Publication number: 20130345119Abstract: The present invention provides an antimicrobial peptide polymer comprising at least one monomer which is digested by pepsin, a multimeric antimicrobial peptide complex comprising the polymer and a cell surface anchoring motif linked to the polymer, an antimicrobial microorganism displaying the multimeric antimicrobial peptide complex, an antimicrobial composition comprising the same, a method of treating an infectious disease caused by bacteria, yeast or fungi by administering the antimicrobial composition, and a method for producing the antimicrobial microorganism. According to the invention, living microorganisms displaying an antimicrobial peptide on the cell surface thereof may be administered in vivo without having to lyse the microbial cell and isolate and purify the antimicrobial peptide, so that the antimicrobial peptide exhibits antimicrobial activity. Thus, the antimicrobial peptide may be produced at significantly reduced costs so that it may have widespread use.Type: ApplicationFiled: December 28, 2010Publication date: December 26, 2013Applicants: INTELLIGENT SYNTHETIC BIOLOGY CENTER, KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGYInventors: Sun-Chang Kim, Ju Ri Shin, Ki Jung Lim, Da Jung Kim, Young Woong Lee, Su A Jang, Bong Hyun Sung
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Publication number: 20130244949Abstract: The present invention relates a prophylactic or therapeutic composition for cancer, and more particularly, to a pro-phylactic or therapeutic composition for cancer comprising a peptide which is represented by an amino acid sequence of the following Formula (I), a method for preventing or treating cancer comprising the step of administering the peptide to a subject, and use of the peptide in the preparation of the prophylactic or therapeutic composition for cancer. (I) APKAMX1LLX2X3L-LX4LQKKGI wherein X1, X2, X3 and X4 are each independently R or K.Type: ApplicationFiled: December 24, 2010Publication date: September 19, 2013Applicant: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGYInventors: Sun Chang Kim, Su A Jang, Da Jung Kim, Bong Hyun Sung, Ki Jeong Lim, Ju Ri Shin, Young Woong Lee
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Patent number: 8242242Abstract: The present invention relates to an artificial transcription factor which can artificially regulate gene expression of an E. coli, wherein the transcription factor comprising zinc finger proteins and transcription factors of prokaryote, and to be engineered E. coli using the same. Specifically, the artificial transcription factors comprising zinc finger domains and transcription factors in E. coli as effector domains are prepared and said artificial transcription library is introduced to E. coli to effectively and artificially regulate gene expression regardless of an activity of endogenous transcription factors in the E. coli and to induce E. coli having various desired phenotypes. Thus, only E. coli having the desired phenotypes useful for industries can be selected and used.Type: GrantFiled: December 15, 2006Date of Patent: August 14, 2012Assignee: Korea Advanced Institute of Science and TechnologyInventors: Sun-Chang Kim, Ju-Young Lee, Bong-Hyun Sung, Jun-Hyoung Lee, Sang-Hee Lee, Kui-Hyeon Kang
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Publication number: 20120088733Abstract: Disclosed are novel antimicrobial peptides which can promote the regeneration of skin cells, thus healing wounds. Pharmaceutical compositions comprising the peptides as active ingredients are also provided for wound healing and skin rejuvenation. The antimicrobial peptides exhibit inhibitory activity against antibiotic-resistant strains, and their antimicrobial activity is maintained without loss of structural stability even under a high salt condition. Also, being proven to promote the migration and regeneration of skin cells in mice as well as in vitro, the antimicrobial peptides may be widely used as an agent for regenerating skin cells. Further, they can find applications in various fields including the medical industry and the cosmetic industry. Hence, the novel antimicrobial peptides are anticipated to have considerable repercussions in the market for antibiotics, wound healing agents and cosmetics.Type: ApplicationFiled: April 27, 2011Publication date: April 12, 2012Applicant: Korea Advanced Institute of Science and TechnologyInventors: Sun-Chang Kim, Da-Jung Kim, Su-A Jang, Bong Hyun Sung, Ki-Jung Lim, Ju-Ri Shin, Young Woong Lee
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Patent number: 8003348Abstract: The present invention relates to a gene construct which is capable of achieving efficient production of an antimicrobial peptide in a microorganism, and a method for efficient mass production and separation of an antimicrobial peptide using the same. The gene construct of the present invention has a translationally coupled configuration of two independent and separate cistrons which encode an acidic peptide and a basic antimicrobial peptide, each having an opposite charge, under the control of a single promoter. The translationally coupled acidic peptide and basic antimicrobial peptide undergo charge-charge interaction simultaneously with expression thereof to neutralize the potential cytotoxicity of the antimicrobial peptide, resulting in prevention of antimicrobial peptide-mediated killing of host microorganisms. In addition, a conjugate of the acidic peptide and the antimicrobial peptide can be separated without chemical or enzymatic treatment.Type: GrantFiled: November 19, 2008Date of Patent: August 23, 2011Assignee: Korea Advanced Institute of Science and TechnologyInventors: Sun Chang Kim, Su A Jang, Bong Hyun Sung, Jung Min Kim, Ki Jung Lim, Ju Ri Shin, Ju Young Lee
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Publication number: 20100184949Abstract: The present invention relates to a gene construct which is capable of achieving efficient production of an antimicrobial peptide in a microorganism, and a method for efficient mass production and separation of an antimicrobial peptide using the same. The gene construct of the present invention has a translationally coupled configuration of two independent and separate cistrons which encode an acidic peptide and a basic antimicrobial peptide, each having an opposite charge, under the control of a single promoter. The translationally coupled acidic peptide and basic antimicrobial peptide undergo charge-charge interaction simultaneously with expression thereof to neutralize the potential cytotoxicity of the antimicrobial peptide, resulting in prevention of antimicrobial peptide-mediated killing of host microorganisms. In addition, a conjugate of the acidic peptide and the antimicrobial peptide can be separated without chemical or enzymatic treatment.Type: ApplicationFiled: November 19, 2008Publication date: July 22, 2010Inventors: Sun Chang Kim, Su A. Jang, Bong Hyun Sung, Jung Min Kim, Ki Jung Lim, Ju Ri Shin, Ju Young Lee
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Publication number: 20100136663Abstract: The present invention relates to an artificial transcription factor which can artificially regulate gene expression of an E. coli, wherein the transcription factor comprising zinc finger proteins and transcription factors of prokaryote, and to be engineered E. coli using the same. Specifically, the artificial transcription factors in E. coli as effector domains are prepared and said artificial transcription library is introduced to E. coli to effectively and artificially regulate gene expression regardless of an activity of endogenous transcription factors in the E. coli and to induce E. coli having various desired phenotypes. Thus, only E. coli having the desired phenotypes useful for industries can be selected and used.Type: ApplicationFiled: December 15, 2006Publication date: June 3, 2010Applicant: Korea Advanced Institute of Science and TechnologyInventors: Sun-Chang KIM, Ju-Young Lee, Bong-Hyun Sung, Jun-Hyoung Lee, Sang-Hee Lee, Kui-Hyeon Kang
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Publication number: 20090305421Abstract: Disclosed herein are a recombinant vector for deletion of specific chromosomal regions and a method for deletion of targeted microbial chromosomal regions using the same. Specifically, the recombinant vector comprises an arabinose-inducible promoter; a gene encoding a protein involved in lambda (?)-red recombination; a rhamnose-inducible promoter; and a gene encoding the I-SceI endonuclease. The present invention enables a convenient, rapid and markerless successive deletion of specific genes of microbes, as compared to a conventional method.Type: ApplicationFiled: November 4, 2008Publication date: December 10, 2009Inventors: Sun Chang Kim, Kui Hyeon Kang, Byung Jo Yu, Jun Hyoung Lee, Bong Hyun Sung, Choong Hoon Lee, Sang Hee Lee, Ju Young Lee, Myung Keun Park