Abstract: Protein substrates are provided comprising integrin binding motif and growth factor (GF) binding peptide motif that enable binding to and activation of both a growth factor receptor and an integrin, simultaneously or selectively. The substrate proteins may be in the form of a mussel adhesive protein where extracellular matrix (ECM) protein derived integrin binding peptide motifs and GF binding peptide motifs are recombinantly incorporated into the mussel adhesive protein. Also provided are use of the protein substrate for regulating cellular behavior including cell adhesion, migration, or proliferation which are essential process in bioprocess, wound healing, tissue engineering, and therapeutic application. In other embodiments, the present invention provides a method and composition of stabilizing and protecting a growth factor from protease digestion or internalization into cells (or cellular uptake) in order to maintain the persistent and durable function of growth factors.

Abstract: Provided are a surfactant adhesive protein comprising an amphiphilic peptide, as a surfactant adhesive protein, at the carbon or amine terminal, a silicone oil and an anticancer composition comprising the surfactant adhesive, where the surfactant adhesive enables homogeneous dispersion of hydrophilic or hydrophobic particles in a hydrophobic or hydrophilic solvent on the basis of strong adhesive strength of the mussel adhesive protein, and the surface adhesive can be favorably used as a surface coating agent requiring antibacterial or antiviral functions as well as a cosmetic product or an ink.

Abstract: Disclosed herein are spherical high-density lipoprotein-like nanoparticles (HDL-NP) having a soft material core (e.g., a lipid-conjugated inorganic core). Also disclosed herein are methods for synthesizing the spherical HDL-NPs. Also disclosed herein are methods for treating disorders such as cardiovascular disease, cancer, inflammatory disorders or reducing NF-kB activity with the spherical HDL-NPs.

Abstract: Provided are a surfactant adhesive protein comprising an amphiphilic peptide, as a surfactant adhesive protein, at the carbon or amine terminal, a silicone oil and an anticancer composition comprising the surfactant adhesive, where the surfactant adhesive enables homogeneous dispersion of hydrophilic or hydrophobic particles in a hydrophobic or hydrophilic solvent on the basis of strong adhesive strength of the mussel adhesive protein, and the surface adhesive can be favorably used as a surface coating agent requiring antibacterial or antiviral functions as well as a cosmetic product or an ink.

Abstract: Provided herein is a surfactant adhesive protein comprising an amphiphilic peptide at the carbon or amine terminal of the protein, enabling homogeneous dispersion of several materials in various solvents and coating of the homogeneously dispersed particles on a hydrophilic or hydrophobic surface, particularly the homogeneous dispersion of hydrophilic or hydrophobic particles in a hydrophobic or hydrophilic solvent on the basis of strong adhesive strength of a mussel adhesive protein; the surface adhesive protein can be used as a surface coating agent requiring antibacterial or antiviral functions as well as a cosmetic product or an ink.

Abstract: Described are methods and devices for enrichment and in situ expansion of circulating tumor cells (CTCs) from biological samples. The methods may include detecting at least one or more of cell adhesion molecules as epithelial mesenchymal transition (EMT) biomarker. Also described is device for detecting or enriching CTCs. The surface of the device may provide at least one or more of cell binding ligands such as ECM or cadherin derived peptide motif to detect the EMT biomarker. Also described is a surface to remove leukocytes from biological samples, leading to efficient enrichment of CTCs from the biological samples.

Abstract: Provided is a method for separating and purifying a mussel adhesive protein, including the steps of: (1) crushing cells containing a mussel adhesive protein; (2) centrifuging the crushed cells to obtain an insoluble protein aggregate including the mussel adhesive protein; (3) treating the insoluble protein aggregate with an acidic organic solvent to obtain a low-purity mussel adhesive protein solution; (4) selectively precipitating the mussel adhesive protein by controlling the acidity of the low-purity mussel adhesive protein solution; and (5) treating the precipitate with a surfactant to remove endotoxins from the mussel adhesive protein. The method of the subject matter can purify a large amount of mussel adhesive proteins of high purity with a simple process. In particular, the subject matter can be applied effectively to the development of novel uses of mussel adhesive proteins by significantly reducing production costs through economical production of the mussel adhesive protein.

Abstract: The present disclosure relates to a three-dimensional micro-environment structure for controlling cell behavior, a three-dimensional surface and an array for controlling cell behavior, and a method for manufacturing the three-dimensional micro-environment structure.

Abstract: Provided herein is a surfactant adhesive protein comprising an amphiphilic peptide at the carbon or amine terminal of the protein, enabling homogeneous dispersion of several materials in various solvents and coating of the homogeneously dispersed particles on a hydrophilic or hydrophobic surface, particularly the homogeneous dispersion of hydrophilic or hydrophobic particles in a hydrophobic or hydrophilic solvent on the basis of strong adhesive strength of a mussel adhesive protein; the surface adhesive protein can be used as a surface coating agent requiring antibacterial or antiviral functions as well as a cosmetic product or an ink.

Abstract: The present invention provides solid supports comprising a surface bound array of dendrons and methods for using the same.

Abstract: The present invention provides solid supports comprising a surface bound array of dendrons and methods for using the same.

Abstract: The present application discloses a substrate that includes a molecular layer of regularly spaced size-controlled macromolecules comprising a polymer comprising branched and linear regions in which a plurality of termini on the branched region are bound to the substrate, and a terminus of the linear region is functionalized.

Abstract: The present patent application describes a cantilever for atomic force microscopy (AFM), which includes a cantilever body having a fixed end and a free end, the free end having a surface region being chemically modified by a dendron in which a plurality of termini of the branched region of the dendron are bound to the surface, and a terminus of the linear region of the dendron is functionalized.

Abstract: The present invention provides for a biochemically and physically defined extracellular microenvironment prepared from mussel adhesive proteins recombinantly functionalized with a variety of bioactive peptides such as extracellular matrix-derived or growth factor-derived peptides. The synthetic extracellular microenvironment can be customized to regulate cellular behavior such as cell adhesion, growth, differentiation and morphogenesis in a variety of cells. The invention provides for a modulatory extracellular microenvironment by presenting a matricryptic site into said mussel adhesive proteins. The invention also provides for devices and methods for screening for optimal combinations of ECM derived peptide motifs in order to create a microenvironment that can regulate specific cellular behavior.

Abstract: The present invention relates to methods for producing an oligonucleotide on a solid substrate surface and methods for using the same. Some aspects of the invention provide methods for selecting a single DNA molecule reproducibily with an atomic force microscope (AFM).

Abstract: The present patent application describes a cantilever for atomic force microscopy (AFM), which includes a cantilever body having a fixed end and a free end, the free end having a surface region being chemically modified by a dendron in which a plurality of termini of the branched region of the dendron are bound to the surface, and a terminus of the linear region of the dendron is functionalized.

Abstract: The present patent application describes a cantilever for atomic force microscopy (AFM), which includes a cantilever body having a fixed end and a free end, the free end having a surface region being chemically modified by a dendron in which a plurality of termini of the branched region of the dendron are bound to the surface, and a terminus of the linear region of the dendron is functionalized.

Abstract: The present invention relates to a method for the synthesis of a polynucleotide on a modified surface of a substrate, wherein the modified surface is obtained by chemically modifying with macromolecules in which a plurality of termini of the branched region are bound to the surface and a terminus of the linear region is functionalized.

Abstract: The present invention provides solid substrates comprising a small number of molecules, for example, ten or less molecules on the convex surface, e.g., on the apex, and methods for producing and using the same.

Abstract: The present application discloses a method for detecting a presence of target ligand in a fluid medium which includes the steps of: (i) contacting the fluid medium with a solid substrate that includes an array of dendrons on its surface, wherein each of the dendron includes a central atom, a probe that is attached to the central atom optionally through a linker, and a base portion attached to the central atom and having a plurality of termini that are attached to the surface of the solid support; and (ii) determining the presence of a probe-target ligand complex by measuring binding force between the bound ligand and detection molecule tethered to the tip of an atomic force microscope (“AFM”), which detection molecule has affinity for the ligand, wherein measurement of an increase in force between the probe-target ligand complex and the detection molecule by AFM indicates the presence of the probe-target ligand complex.