Abstract: This disclosure relates to an apparatus and method for sorting motile sperms from crude semen. the enrichment of a population of spermatozoa with capacitated spermatozoa can be achieved by providing a 1-to-4-degree vertical temperature difference in combination with the swim-up method.

Abstract: A sperm sorting method is provided. The sperm sorting method includes the following steps: providing a porous layer with a first side and a second side opposite to the first side; disposing an unsorted sperm group at the second side; forming a temperature difference between the first side and the second side; and collecting a sorted sperm group which arrives the first side and passes through the porous layer from the second side based on the temperature difference and overcoming gravity.

Abstract: A sperm sorting device adapted to collaborate with a temperature controlling component is provided. The sperm sorting device includes an upper portion, a lower portion and a porous layer. The upper portion is formed with an upper chamber. The lower portion is formed with a lower chamber. The upper chamber and the lower chamber are disposed vertically. The temperature controlling component is adapted to be disposed outside the upper portion or the lower portion to make the temperature of the upper chamber higher than the lower chamber. The porous layer is disposed between the upper chamber and the lower chamber. The porous layer is adapted to allow sperms to pass through from the lower chamber to the upper chamber and adapted to form a temperature difference between the upper chamber and the lower chamber. Besides, a temperature difference device adapted for the sperm sorting device is also provided.

Abstract: An impedance-type chip for real-time sensing sweat pressure, a micro-control system, and method thereof are provided for monitoring a physiological state of a subject. The impedance-type chip includes a substrate, a pair of comb-shaped electrodes, a first double-layered junction plate, a microfluidic channel plate, a second double-layered junction plate, and a sealing plate. Each the comb-shaped electrodes has a plurality of sub-electrodes and is disposed on the substrate to provide different impedance values. The first double-layered junction plate is disposed on the substrate, the microfluidic channel plate is disposed on the first double-layered junction plates, and the second double-layered junction plate is disposed on the microfluidic channel plate, wherein the first double-layered junction plate, the microfluidic channel plate, and the second double-layered junction plate have a microfluidic channel with a cavity.

Abstract: The present invention provides a sperm sorting chip and a method for sorting sperm using the same. Said sperm sorting chip includes: a flow channel structure sequentially configured with a gradually diverging flow field region, a main flow channel, and a gradually converging main flow channel intercommunicated with each other from a first side end to a second side end; a fluid injection port, a semen injection port, and a semen extraction port separately located at the first side end and communicated with a main input channel of the gradual diverging flow field region; and a waste fluid outlet located at the second side end and communicated with the gradually converging main flow channel. The gradually diverging flow field region further includes a plurality of sub-input channels derived from the main input channel and converged into the main flow channel, and the plurality of sub-input channels have a gradually widening channel width at the junction with the main flow channel.

Abstract: An impedance-type chip for real-time sensing sweat pressure, a micro-control system, and method thereof are provided for monitoring a physiological state of a subject. The impedance-type chip includes a substrate, a pair of comb-shaped electrodes, a first double-layered junction plate, a microfluidic channel plate, a second double-layered junction plate, and a sealing plate. Each the comb-shaped electrodes has a plurality of sub-electrodes and is disposed on the substrate to provide different impedance values. The first double-layered junction plate is disposed on the substrate, the microfluidic channel plate is disposed on the first double-layered junction plates, and the second double-layered junction plate is disposed on the microfluidic channel plate, wherein the first double-layered junction plate, the microfluidic channel plate, and the second double-layered junction plate have a microfluidic channel with a cavity.

Abstract: The present invention provides a microfluidic structure for sorting targeted substances. The microfluidic structure includes: an inlet portion having at least one fluid input port; an outlet portion having a plurality of fluid output ports; a first annular flow channel communicated with the inlet portion at the upstream end and rotatably extended; and a second annular flow channel communicated with the downstream end of the first annular flow channel at the upstream end, communicated with the outlet portion at the downstream end and rotatably extended. The first annular flow channel and the second annular flow channel are connected in series according to an S-shaped track, so that the outer side wall of the first annular flow channel is continuously connected to the inner side wall of the second annular flow channel. The cross-sections of the first annular flow channel and the second annular flow channel have a height difference.

Abstract: An embodiment of this invention A NiO chip is provided. The NiO chip includes a substrate, a nickel oxide thin film on the substrate, and a bioprobe layer on the nickel oxide thin film. The nickel oxide thin film has a light transmittance of more than 60% and a nanostructure. The bioprobe layer includes a plurality of bioprobes modified by Histidine (His) or a His-tagged protein.

Abstract: The present invention provides a sperm sorting chip and a method for sorting sperm using the same. Said sperm sorting chip includes: a flow channel structure sequentially configured with a gradually diverging flow field region, a main flow channel, and a gradually converging main flow channel intercommunicated with each other from a first side end to a second side end; a fluid injection port, a semen injection port, and a semen extraction port separately located at the first side end and communicated with a main input channel of the gradual diverging flow field region; and a waste fluid outlet located at the second side end and communicated with the gradually converging main flow channel. The gradually diverging flow field region further includes a plurality of sub-input channels derived from the main input channel and converged into the main flow channel, and the plurality of sub-input channels have a gradually widening channel width at the junction with the main flow channel.

Abstract: A microfluidic chip for sorting sperm includes a substrate, a sample channel, and a plurality of divergent channels. The sample channel is disposed in the substrate. The divergent channels are disposed in the substrate. Each of the divergent channels includes a main channel and two branch channels. The two branch channels are connected to an end of the main channel away from the sample channel. The two branch channels are disposed at two sides of the main channels respectively. The sample channel and the main channels of the plurality of divergent channels are substantially connected to each other in serial along a direction.

Abstract: A biological sensing device and a method for separating a biomolecule are provided. The biological sensing device includes an amino acid sequence and a signal-generating unit. The amino acid sequence includes SEQ ID NO: 1 or SEQ ID NO: 2, and is for binding with UlaG protein labeled on a biomolecule. The signal-generating unit connects to the amino acid sequence.

Abstract: A biological sensing device and a method for separating a biomolecule are provided. The biological sensing device includes an amino acid sequence and a signal-generating unit. The amino acid sequence includes SEQ ID NO: 1 or SEQ ID NO: 2, and is for binding with UlaG protein labeled on a biomolecule. The signal-generating unit connects to the amino acid sequence.

Abstract: A biological sensing device and a method for separating a biomolecule are provided. The biological sensing device includes an amino acid sequence and a signal-generating unit. The amino acid sequence includes SEQ ID NO: 1 or SEQ ID NO: 2, and is for binding with UlaG protein labeled on a biomolecule. The signal-generating unit connects to the amino acid sequence.