Patents by Inventor Bosiljka Tasic
Bosiljka Tasic has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240254514Abstract: Artificial expression constructs for modulating gene expression in targeted central nervous system cell types are described. The artificial expression constructs can be used to express synthetic genes or modify gene expression in GABAergic or glutamatergic neurons within the thalamus. In some cases, the artificial constructs can also be used to express synthetic genes or modify gene expression in neurons within the thalamus as well as a secondary cell type.Type: ApplicationFiled: May 20, 2022Publication date: August 1, 2024Applicant: ALLEN INSTITUTEInventors: Tanya Daigle, Edward Sebastian Lein, Boaz P. Levi, John K. Mich, Amanda Mitchell, Bosiljka Tasic, Jonathan Ting, Natalie Weed, Hongkui Zeng
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Publication number: 20240182923Abstract: Artificial expression constructs for modulating gene expression in targeted central nervous system cell types are described. The artificial expression constructs can be used to express synthetic genes or modify gene expression in claustrum neurons including L6 IT Car3 neurons.Type: ApplicationFiled: March 30, 2022Publication date: June 6, 2024Applicant: Allen InstituteInventors: Tanya Daigle, Lucas T. Graybuck, Adriana Estela Sedeño Cortés, Bosiljka Tasic, Jonathan Ting
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Publication number: 20240018543Abstract: Artificial expression constructs for modulating gene expression in targeted central nervous system cell types are described. The artificial expression constructs can be used to express synthetic genes or modify gene expression in chandelier cells. Chandelier cells are a subtype of GABAergic interneurons that that have been implicated in disorders such as epilepsy and schizophrenia.Type: ApplicationFiled: November 10, 2021Publication date: January 18, 2024Applicant: Allen InstituteInventors: Tanya Daigle, Lucas T. Graybuck, Brian Edward Kalmbach, Edward Sebastian Lein, John K. Mich, Boaz P. Levi, Adriana Estela Sedeño Cortés, Bosiljka Tasic, Jonathan Ting, Hongkui Zeng
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Publication number: 20230302158Abstract: Artificial expression constructs for modulating gene expression in striatal neurons are described. The artificial expression constructs can be used to express heterologous genes in striatal neurons including in striatal medium spiny neuron-pan, striatal medium spiny neuron-indirect pathway, striatal medium spiny neuron-direct pathway, striatal interneuron-cholinergic, and Drd3+ medium spiny neurons in olfactory tubercle. The artificial expression constructs can be used for many purposes, including to research and treat movement disorders such as Parkinson's disease and Huntington's disease.Type: ApplicationFiled: August 13, 2021Publication date: September 28, 2023Applicant: Allen InstituteInventors: Tanya Daigle, Edward Sebastian Lein, Boaz P. Levi, John K. Mich, Bosiljka Tasic, Jonathan Ting, Hongkui Zeng
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Publication number: 20230212608Abstract: Artificial expression constructs for selectively modulating gene expression in selected central nervous system cell types are described. The artificial expression constructs can be used to selectively express synthetic genes or modify gene expression in inhibitory neocortical GABAergic neurons including somatostatin GABAergic neurons, parvalbumin GABAergic neurons, vasointestinal peptide GABAergic neurons, Lamp5 GABAergic neurons, and in some instances astrocytes.Type: ApplicationFiled: June 4, 2021Publication date: July 6, 2023Applicant: Allen InstituteInventors: Tanya Daigle, Lucas T. Graybuck, Edward Sebastian Lein, Boaz P. Levi, John K. Mich, Adriana Estela Sedeño Cortés, Bosiljka Tasic, Jonathan Ting, Miranda Walker, Hongkui Zeng
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Publication number: 20230159952Abstract: Artificial expression constructs for selectively modulating gene expression in selected central nervous system cell types are described. Particularly, the artificial expression constructs can be used to selectively express synthetic genes and/or modify gene expression in neocortical glutamatergic layer 5 neurons, such as glutamatergic layer 5 extratelencephalic-projecting (L5 ET) neurons.Type: ApplicationFiled: April 21, 2021Publication date: May 25, 2023Applicant: ALLEN INSTITUTEInventors: Tanya Daigle, Lucas T. Graybuck, Brian Edward Kalmbach, Edward Sebastian Lein, Boaz P. Levi, John K. Mich, Adriana Estela Sedeño Cortés, Bosiljka Tasic, Jonathan Ting, Hongkui Zeng
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Publication number: 20230117172Abstract: Artificial expression constructs for selectively modulating gene expression in selected central nervous system cell types are described. The artificial expression constructs can be used to selectively express synthetic genes or modify gene expression in astrocytes, oligodendrocytes, microglia, pericytes, SMC, or endothelial cells.Type: ApplicationFiled: March 26, 2021Publication date: April 20, 2023Applicant: ALLEN INSTITUTEInventors: Jonathan Ting, Bosiljka Tasic, Boaz P. Levi, Tanya Daigle, Lucas T. Graybuck, Edward Sebastian Lein, John K. Mich, Adriana Estela Sedeño Cortés, Hongkui Zeng
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Publication number: 20220249703Abstract: Artificial expression constructs for selectively modulating gene expression in selected central nervous system cell types are described. The artificial expression constructs can be used to selectively express synthetic genes or modify gene expression in GABAergic neurons generally; and/or GABAergic neuron cell types such as lysosomal associated membrane protein 5 (Lamp5) neurons; vasoactive intestinal polypeptide-expressing (Vip) neurons; somatostatin (Sst) neurons; and/or parvalbumin (Pvalb) neuron cell types. Certain artificial expression constructs additionally drive selective gene expression in Layer 4 and/or layer 5 intratelencephalic (IT) neurons, deep cerebellar nuclear neurons or cerebellar Purkinje cells.Type: ApplicationFiled: February 14, 2020Publication date: August 11, 2022Applicant: Allen InstituteInventors: Jonathan Ting, Boaz P. Levi, Bosiljka Tasic, John K. Mich, Erik Hess, Edward Sebastian Lein, Lucas T. Graybuck, Tanya Daigle, Hongkui Zeng
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Publication number: 20210395780Abstract: Artificial expression constructs for selectively modulating gene expression in selected central nervous system cell types are described. The artificial expression constructs can be used to selectively express synthetic genes or modify gene expression in excitatory cortical neurons, such as primarily within cortical layers 2/3, 4, 5, and 6 and including those with extratelencephalic (ET) projections, intratelencephalic (IT) projections, and pyramidal tract (PT) projections, among others.Type: ApplicationFiled: November 5, 2019Publication date: December 23, 2021Applicant: Allen InstituteInventors: Lucas T. Graybuck, Bosiljka Tasic, Tanya Daigle, Jonathan Ting, Hongkui Zeng, Brian Edward Kalmbach, John K. Mich, Erik Hess, Edward Sebastian Lein, Boaz P. Levi
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Patent number: 9932607Abstract: Methods for inserting a polynucleotide sequence into the genome of a human cell are provided. The present methods result in insertion of a polynucleotide sequence of interest into the H11 locus in the genome of a human cell. Also provided are nucleic acids that include sequences for integrating a polynucleotide sequence of interest into the H11 locus in the genome of a human cell. A transgenic human cell including site specific recombination sites at the H11 locus is also disclosed.Type: GrantFiled: November 12, 2014Date of Patent: April 3, 2018Assignee: The Board of Trustees of the Leland Stanford Junior UniversityInventors: Michele Pamela Calos, Ruby Yanru Tsai, Fangfang Zhu, Matthew Gamboa, Alfonso P. Farruggio, Simon Hippenmeyer, Bosiljka Tasic, Birgitt Schüle
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Patent number: 9125385Abstract: The present disclosure provides a method of making a mammal (e.g., a rodent, such as a mouse) by integrating an intact polynucleotide sequence into a specific genomic locus of the mammal to result in a transgenic mammal. A transgenic mammal made by the methods of the present disclosure would contain a known copy number (e.g., one) of the inserted polynucleotide sequence at a predetermined location. The method involves introducing a site-specific recombinase and a targeting construct, containing a first recombination site and the polynucleotide sequence of interest, into the mammalian cell. The genome of the cell contains a second recombination site and recombination between the first and second recombination sites is facilitated by the site-specific, uni-directional recombinase. The result of the recombination is site-specific integration of the polynucleotide sequence of interest in the genome of the mammal. This inserted sequence is then also transmitted to the progeny of the mammal.Type: GrantFiled: November 10, 2011Date of Patent: September 8, 2015Assignee: The Board of Trustees of the Leland Stanford Junior UniversityInventors: Liqun Luo, Ruby Yanru Tsai, Bosiljka Tasic, Simon Hippenmeyer, Hui Zong
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Publication number: 20150140665Abstract: Methods for inserting a polynucleotide sequence into the genome of a human cell are provided. The present methods result in insertion of a polynucleotide sequence of interest into the H11 locus in the genome of a human cell. Also provided are nucleic acids that include sequences for integrating a polynucleotide sequence of interest into the H11 locus in the genome of a human cell. A transgenic human cell including site specific recombination sites at the H11 locus is also disclosed.Type: ApplicationFiled: November 12, 2014Publication date: May 21, 2015Inventors: Michele Pamela Calos, Ruby Yanru Tsai, Fangfang Zhu, Matthew Gamboa, Alfonso P. Farruggio, Simon Hippenmeyer, Bosiljka Tasic, Birgitt Schüle
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Publication number: 20120124686Abstract: The present disclosure provides a method of making a mammal (e.g., a rodent, such as a mouse) by integrating an intact polynucleotide sequence into a specific genomic locus of the mammal to result in a transgenic mammal. A transgenic mammal made by the methods of the present disclosure would contain a known copy number (e.g., one) of the inserted polynucleotide sequence at a predetermined location. The method involves introducing a site-specific recombinase and a targeting construct, containing a first recombination site and the polynucleotide sequence of interest, into the mammalian cell. The genome of the cell contains a second recombination site and recombination between the first and second recombination sites is facilitated by the site-specific, uni-directional recombinase. The result of the recombination is site-specific integration of the polynucleotide sequence of interest in the genome of the mammal. This inserted sequence is then also transmitted to the progeny of the mammal.Type: ApplicationFiled: November 10, 2011Publication date: May 17, 2012Inventors: Liqun Luo, Ruby Yanru Tsai, Bosiljka Tasic, Simon Hippenmeyer, Hui Zong
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Publication number: 20110277050Abstract: Embodiments of the present invention describe a novel and versatile inducible binary expression system (the ‘Q system’) and methods for controlling transgene expression in vitro and in vivo, for lineage tracing, for genetic mosaic analysis and for determining gene function.Type: ApplicationFiled: March 11, 2011Publication date: November 10, 2011Applicant: The Board of Trustees of the Leland Stanford Junior UniversityInventors: Liqun Luo, Christopher Potter, Bosiljka Tasic