Abstract: The invention provides novel and improved methods that allow effective capture of valuable active ingredients in biomass (e.g., DDGS) at cost-effective commercial scale. The invention also provides novel compositions of active ingredients with unique properties (e.g., nutritional values and enhanced bioavailability).

Abstract: A method for assaying the antioxidant capacity of a skin care product, the method including preparing an emulsion base, dissolving a sample of a skin care product into the emulsion base to form a homogeneous emulsion mixture, adding a detection probe to the homogeneous emulsion mixture, adding reactive oxygen species generator and/or reactive nitrogen species generator to the homogeneous emulsion mixture, measuring the fluorescence intensity change of the detection probe in the presence of the sample over time, in the presence of the standard over time, and in the presence of a blank over time, and calculating the initial rate of oxidation of the detection probe to determine the antioxidant capacity of the sample of the skin care product.

Abstract: A method for assaying the antioxidant capacity of a skin care product, the method including preparing an emulsion base, dissolving a sample of a skin care product into the emulsion base to form a homogeneous emulsion mixture, adding a detection probe to the homogeneous emulsion mixture, adding reactive oxygen species generator and/or reactive nitrogen species generator to the homogeneous emulsion mixture, measuring the fluorescence intensity change of the detection probe in the presence of the sample over time, in the presence of the standard over time, and in the presence of a blank over time, and calculating the initial rate of oxidation of the detection probe to determine the antioxidant capacity of the sample of the skin care product.

Abstract: A kit for assaying the antioxidant capacity of a sample, the kit including an extraction solution including a solubility enhancing compound to be added to a sample for extracting antioxidants present in the sample; and a fluorescent probe to be added to the extract.

Abstract: A method of assaying the antioxidant capacity of a sample, the method including preparing an extraction solution including a solubility enhancing compound, adding the sample to the extraction solution, extracting the antioxidants present in the sample, adding a fluorescent probe to the extract, adding a free radical generator to the extract, detecting the fluorescence intensity decay of the probe in the presence of the sample over time, and calculating the antioxidant capacity of the sample based on the fluorescence intensity decay of the probe in the presence of the sample.

Abstract: A method of assaying the reactive oxidants present in a smoke sample, the method comprising: preparing solution including a reductant; passing smoke through the solution; detecting the concentration changes of the probe in the presence of the smoke sample over time; and calculating the concentration of reactive oxidants of the smoke sample from the concentration changes of the reductant in the presence of the smoke sample. A method of assaying the reactive oxidants present in a smoke sample, the method comprising: preparing a solid material containing a reductant; passing smoke through the solid material; detecting the concentration changes of the reductant in the presence of the smoke sample over time; and calculating the concentration of reactive oxidants of the smoke sample from the concentration changes of the reductant in the presence of the smoke sample.

Abstract: A method of assaying the antioxidant capacity of a sample, the method including preparing an extraction solution including a solubility enhancing compound, adding the sample to the extraction solution, extracting the antioxidants present in the sample, adding a fluorescent probe to the extract, adding a free radical generator to the extract, detecting the fluorescence intensity decay of the probe in the presence of the sample over time, and calculating the antioxidant capacity of the sample based on the fluorescence intensity decay of the probe in the presence of the sample.