Abstract: The present invention relates to methods modifying cell surface markers of red blood cells (RBCs) and uses of the same. In particular, the method comprises contacting an RBC with a peptide in the presence of a ligase, under suitable conditions and for sufficient time to allow ligation of the peptide to the RBC to form an RBC-peptide conjugate. In one embodiment, the ligase is OaAEPI ligase. The RBC-peptide conjugate may be further contacted with an effector molecule under suitable conditions and for sufficient time for conjugation of the effector molecule to the RBC-peptide to form an RBC-peptide-effector molecule conjugate.

Abstract: The invention relates to the use of red blood cells extracellular vesicle (RBCEV) to deliver nucleic acids to immune cells (e.g. T cells), wherein the nucleic acid cargoes are loaded into the RBCEV in vitro or ex vivo. The invention also relates to the prophylactic and therapeutic use of said immune cells in diseases, such as cancer.

Abstract: Amendment to the Abstract: Disclosed are an in-place supervision method and apparatus suitable for overhead transmission line maintenance operation, the method includes: geographic coordinate information of an overhead transmission line is, written into a risk management and control master station system of the whole electric power operation process, and an in-place supervision algorithm is written into the risk management and control master station system of the whole electric power operation process; geodetic coordinates A of a maintenance operation site and geodetic coordinates B of a supervisor are read; a distance is solved based on an elliptic spherical arc length approximate chord length algorithm; whether the distance S exceeds a threshold value of out of supervision or not is judged.