Patents by Inventor Boya ZHOU

Boya ZHOU has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Glycosyltransferase mutant, and method for catalytic synthesis of rebaudioside m using glycosyltransferase mutant
    Patent number: 12735684
    Abstract: A glycosyltransferase mutant and a method for catalytic synthesis of rebaudioside M using the glycosyltransferase mutant are provided. The glycosyltransferase mutant is obtained through mutation based on a glycosyltransferase amino acid sequence shown in SEQ ID NO: 1, and can be produced by inducing expression in a mutant strain. The glycosyltransferase mutant can be used as a catalyst for enzymatic catalytic synthesis of 12.8 g/LRebM from 20 g/LRebE. Michaelis constants (a kinetic parameter) of the glycosyltransferase mutant S195Q for rebaudioside E and rebaudioside D are 56.34±2.02 ?M and 214.48±14.54 ?M, respectively, which are ? and ? of a Michaelis constant of a wild-type (WT) glycosyltransferase, respectively.
    Type: Grant
    Filed: July 29, 2022
    Date of Patent: September 15, 2026
    Assignee: XINGHUA GL STEVIA CO., LTD
    Inventors: Boya Zhou, Honghua Jia, Jing Wang, Yan Li, Yehui Tao, Jie Yu, Lei Lin, Ruiqi Ma
  • GLYCOSYLTRANSFERASE MUTANT, AND METHOD FOR CATALYTIC SYNTHESIS OF REBAUDIOSIDE M USING GLYCOSYLTRANSFERASE MUTANT
    Publication number: 20240287479
    Abstract: A glycosyltransferase mutant and a method for catalytic synthesis of rebaudioside M using the glycosyltransferase mutant are provided. The glycosyltransferase mutant is obtained through mutation based on a glycosyltransferase amino acid sequence shown in SEQ ID NO: 1, and can be produced by inducing expression in a mutant strain. The glycosyltransferase mutant can be used as a catalyst for enzymatic catalytic synthesis of 12.8 g/LRebM from 20 g/LRebE. Michaelis constants (a kinetic parameter) of the glycosyltransferase mutant S195Q for rebaudioside E and rebaudioside D are 56.34±2.02 ?M and 214.48±14.54 ?M, respectively, which are ? and ? of a Michaelis constant of a wild-type (WT) glycosyltransferase, respectively.
    Type: Application
    Filed: July 29, 2022
    Publication date: August 29, 2024
    Applicant: XINGHUA GL STEVIA CO., LTD
    Inventors: Boya ZHOU, Honghua JIA, Jing WANG, Yan LI, Yehui TAO, Jie YU, Lei LIN, Ruiqi MA