Patents by Inventor Boyd Hardesty

Boyd Hardesty has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Method of synthesizing a fluorescently labeled protein
    Patent number: 6448033
    Abstract: The present invention provides a method of synthesizing a fluorescently labeled protein in a cell free protein synthesis system, comprising the steps of: (a) incubating a sample of ribosomes obtained from a cell-free extract with plasmid DNA containing a coding sequence for a protein of interest, said sample incubated in a coupled transcription/translation medium together with a aminoacyl tRNA having fluorescent label; (b) partially purifying said fluorescently labeled protein by separating newly synthesized, fluorescently-labeled protein from other fluorescent components within said sample; (c) measuring the amount of protein synthesized; (d) determining fluorescence of newly synthesized protein; and (e) determining the biological activity of the newly synthesized protein.
    Type: Grant
    Filed: January 24, 1996
    Date of Patent: September 10, 2002
    Assignee: Research Development Foundation
    Inventors: Wieslaw Kudlicki, Gisela Kramer, Boyd Hardesty
  • Synthetic TRNAS
    Patent number: 5358862
    Abstract: Synthetic tRNAs are produced from tRNA.sup.Cys (AAA), tRNA.sup.Ser (AAA), tRNA.sub.e.sup.Ala (AAA), and tRNA.sub.i.sup.Ala (AAA) genes. Polyuridylic acid-dependent syntheses of polypeptides were carried out in vitro on E. coli ribosomes using the synthetic tRNAs.
    Type: Grant
    Filed: July 14, 1992
    Date of Patent: October 25, 1994
    Assignee: Research Development Foundation
    Inventors: Boyd Hardesty, Wendy L. Picking