Abstract: A UV chamber for inactivating microorganisms in an agricultural facility and method of enhancing sanitation in an agricultural facility using the same. Items are placed inside the UV chamber when entering an agricultural facility. The chamber locks enclosing the item therein and a lighting device directs UV radiation toward the item for a predetermined amount of time necessary to inactivate any microorganisms on the items. The inactive microorganisms are then exposed to the animals within the facility so the animals can build immunity to the microorganism.