Abstract: An immunogenic composition is provided herein. The immunogenic compositions are used to identify and select immunogenic antigens that elicit immune responses in a subject and may be subsequently used in multi-antigen vaccine compositions against one or more diseases or conditions. According to some embodiments, the immunogenic composition may include a plurality of nucleic acid fragments or minigenes derived from a nucleic acid library, wherein each nucleic acid fragment encodes a different antigen or functional portion thereof, and wherein the different antigens or functional portions thereof are associated with one or more disease or condition. The immunogenic composition may also include a delivery medium loaded with the plurality of nucleic acid fragments and in some embodiments, the delivery medium is loaded with nucleic acid fragments in such a way that individual antigen presenting cells receive only a subset of the nucleic acids within a vaccine in order to minimize antigenic competition.

Abstract: In one aspect, the present disclosure provides a trapping vaccine composition comprising a trapping antigenic component, a protective component, and a liver cell-targeting component, wherein the trapping antigenic component comprises a nucleic acid molecule or a protein, the protective component comprises a synthetic or non-natural molecule or formation of synthetic or non-natural molecules, and wherein the liver cell-targeting component is capable of delivering the vaccine composition to a liver cell or liver tissue.

Abstract: Compositions, methods, and regimens for vaccinating subjects against malaria parasites are provided herein. The compositions include first and second compositions having priming and/or trapping components. The priming components include, but are not limited to, DNA or RNA polynucleotides, and the trapping components include, but are not limited to, DNA or RNA polynucleotides, one or more attenuated sporozoites and/or other liver-specific antigens as viral or other formulations. Following administration of one or more of the compositions provided herein using one or more of the vaccination regimens and/or methods, high levels of liver resident memory CD8+ T cells are induced in the subjects leading to protection against sporozoite challenge.

Abstract: The present invention provides methods and compositions for the diagnosis of hyperproliferative disease and autoimmune disease. Tumor associated antigens, nucleic acids encoding them and antibodies to the tumor associated antigens are provided for the diagnosis of hyperproliferative disease, such as, for example, ovarian cancer, breast cancer, lung cancer, colorectal cancer, and other epithelial cancers, and for the diagnosis of autoimmune disease.