Abstract: Through a combination of apoptosis, necrosis, and secretion, tumor DNA is released into the bloodstream and becomes part of the cell-free DNA (cfDNA). The present disclosure provides methods, systems, devices, and kits for isolating circulating free DNA (cfDNA) from a biological sample (e.g., plasma) using RNA-guided DNA binding proteins (e.g. Gas proteins.). Particularly, the disclosure provide methods, systems, devices, and kits for Cas9 mediated capture of cfDNA from flowing plasma.

Abstract: Novel methods for diagnosing and treating diffuse large B-cell lymphoma (DLBCL) and/or primary central nervous system diffuse large B-cell lymphoma (PCNSL) are provided herein. The present invention provides a set of methylation markers which were identified in silico and confirmed in archival and tissue samples could achieve 100% accuracy to discriminate DLBCL and/or PCNSL from other CNS neoplasms. The markers can be identified using QM-MSP and a novel simpler, faster, qMSP assay called TAM-MSP.

Abstract: In accordance with some embodiments, the present invention comprises use of a rapid and accurate QM-MSP and cMethDNA methylation marker-based assays to quickly distinguish between cancer and benign/normal tissues in a biological sample from a subject suspected of having cancer. Methods for detecting breast, colon, and lung cancers in biological samples of suspect tissues and fluids are also provided to assist in triaging subjects suspected of having cancer for expedited biopsy and pathology review in low resource settings.

Abstract: A biofilm resistant fluid handling system is provided having at least one structural component adapted to retain or deliver fluid. The at least one structural component is formed from a biofilm resistant material comprising polymeric resin and an inorganic carrier conjugated with a biocidal metal ion. When a surface of the at least one structural component is exposed to a continuous or intermittent aqueous solution environment the formation of active biofilm on the surface is prevented and planktonic bacteria in proximity of said surface is killed. Additionally, methods for extending the biocidal properties of a biofilm resistant fluid handling system are provided.

Abstract: A biofilm resistant fluid handling system is provided having at least one structural component adapted to retain or deliver fluid. The at least one structural component is formed from a biofilm resistant material comprising polymeric resin and an inorganic carrier conjugated with a biocidal metal ion. When a surface of the at least one structural component is exposed to a continuous or intermittent aqueous solution environment the formation of active biofilm on the surface is prevented and planktonic bacteria in proximity of said surface is killed. Additionally, methods for extending the biocidal properties of a biofilm resistant fluid handling system are provided.