Abstract: Disclosed herein are formulations comprising biologically prepared fibers and methods of preparing said formulations. These formulations can be utilized for repair of structures comprised of a variety of materials, including cementitious material, natural rock, mortar, and concrete. The formulation includes biologically prepared fibers, and a solvent or carrier solution. The formulation may additionally and optionally incorporate at least one enzyme. Once the formulation containing the biologically prepared fibers is applied to a void or crack within the structure, the fibers form a three-dimensional fibrous scaffold configuration in the void of the structure.

Abstract: Provided are Cry3Aa protein crystals comprising a negatively-charged biomolecule encapsulated therein. The solvent channel of the Cry3Aa protein crystal comprises one or more amino acid substitutions of an uncharged or negatively charged amino acid for a positively charged amino acid. Encapsulation of the biomolecule comprises interaction of the biomolecule with the one or more positively charged amino acids in the solvent channel. Negatively-charged biomolecules of interest include nucleic acids and negatively-charged proteins. In some instances, the negatively-charged biomolecule is an RNA (e.g., an siRNA) adapted to reduce expression of a target gene by RNA interference (RNAi). Methods of using the Cry3Aa protein crystals are also provided. For example, provided are methods of controlling a pest, a bacterium, a virus, a fungus, or a parasite using the Cry3Aa protein crystals. Methods of producing the Cry3Aa protein crystals are also provided.

Abstract: It provides novel fusion proteins that are capable of self-crystallization and exhibit improved physical properties such as enhanced tolerance to organic solvents and increased thermostability. Polynucleotides encoding the fusion proteins as well as methods of making and using such fusion proteins are also described.

Abstract: Provide is a novel system for immobilizing re-combinantly produced proteins by entrapping them in crystals of co-expressed proteins that are capable of self-crystallization . Related compositions and as well as methods of making and using the immobilized proteins are also described.

Abstract: It provides novel fusion proteins that are capable of self-crystallization and exhibit improved physical properties such as enhanced tolerance to organic solvents and increased thermostability. Polynucleotides encoding the fusion proteins as well as methods of making and using such fusion proteins are also described.