Abstract: Systems, apparatuses, and methods for implementing dynamic clock control to increase stutter efficiency in a memory subsystem are disclosed. A system includes at least a processor, a memory, and a communication fabric coupled to the processor and memory. The system implements a stutter mode for a first region of the fabric, with stutter mode including an idle state and an active state. Stutter efficiency is defined as the idle time divided by the sum of the active time and the idle time. Reducing the exit latency of going from the idle state to the active state increases the stutter efficiency which increases the power savings achieved by implementing the stutter mode. Since the phase-locked loop (PLL) is one of the main contributors to the exit latency, the PLL is powered down and one or more bypass clocks are provided during the stutter mode.

Abstract: Systems, apparatuses, and methods for implementing dynamic clock control to increase stutter efficiency in a memory subsystem are disclosed. A system includes at least a processor, a memory, and a communication fabric coupled to the processor and memory. The system implements a stutter mode for a first region of the fabric, with stutter mode including an idle state and an active state. Stutter efficiency is defined as the idle time divided by the sum of the active time and the idle time. Reducing the exit latency of going from the idle state to the active state increases the stutter efficiency which increases the power savings achieved by implementing the stutter mode. Since the phase-locked loop (PLL) is one of the main contributors to the exit latency, the PLL is powered down and one or more bypass clocks are provided during the stutter mode.

Abstract: The assembly includes a docking console and a manifold. The docking console includes a cartridge support surface having a first end and a second end. The manifold has one or more wells defined therein. The docking console further includes a manifold retention bracket to releasably hold the manifold against a fluid cartridge supported on the cartridge support surface at an interface position such that the one or more wells are in fluid communication with the fluid cartridge and a biased seal bar to press the fluid cartridge against the manifold held by the manifold retention bracket. A hydrophilic porous frit disposed within at least one of the wells and is to permit liquid to flow through the outlet aperture but prevent gas from passing through the outlet aperture.

Abstract: A mounting alignment apparatus may include a rail assembly including a first pair of alignment surfaces. The mounting alignment apparatus may also include a slide assembly including a second pair of alignment surfaces. The first and second pairs of alignment surfaces may define an interacting capturing geometry therebetween, permitting sliding movement of the slide assembly relative to the rail assembly along a first axis, and restricting movement of the slide assembly relative to the rail assembly about an axis other than the first axis. The mounting alignment apparatus may also include a tension adjustment assembly coupled between the rail assembly and the slide assembly for positioning the slide assembly relative to the rail assembly along the first axis.

Abstract: Detection apparatus includes a microfluorometer having an objective, an excitation radiation source, and a detector. The detection apparatus also includes a fluidic system for delivering reagents from a reagent cartridge to a flow cell. The fluidic system includes a manifold body having a plurality of fluidic channels configured for fluid communication between the reagent cartridge and the flow cell. The fluidic system also includes a plurality of reagent sippers. The fluidic system also includes a valve configured to mediate fluid between reagent reservoirs and the flow cell. The detection apparatus also includes a flow cell latch clamp module having a clamp cover for holding the flow cell. The objective is configured to direct excitation radiation from the radiation source to the flow cell and to direct emission from the flow cell to the detector. The microfluorometer is movable to acquire wide-field images of different areas of the flow cell.

Abstract: An analysis instrument may perform analytical operations on an analyte that is combined with multiple reagents prior to being introduced into a flow cell. The instrument may include a nozzle sipper that aspirates reagents from a recipient, along with an analyte. The reagents may be directed to a volume and may be repeatedly moved into and out of the volume by cycling of a pump. The reagents may be ejected into a destination recipient with the nozzle sipper promoting vorticity in the recipient to enhance mixing. The repeated aspiration and ejection through the nozzle sipper effectively mixes the reagents and the template in an automated or semi-automated fashion.

Abstract: An analysis system may perform operations on an analyte that may be combined with multiple regents prior to being introduced into a flow cell. The instrument may include a volume into which the reagents to be combined with the analyte are aspirated one-by-one. The volume may be formed as a serpentine channel in a valve manifold associated with sippers for aspirating the reagents. The reagents may then be mixed by cycling a pump to move the reagents within the mixing volume or channel.

Abstract: A system may include a flow cell to support analytes of interest; a selector valve coupled to the flow cell to select a flow path through the flow cell from a plurality of flow paths; a pump coupled to the flow cell to displace fluids through the selected flow path during an analysis operation; and control circuitry coupled to the selector valve to command the selector valve to select the selected flow path.

Abstract: Disclosed herein are devices and techniques for providing for enhanced fluid mixing in a sample vial. A plunger head may be removably housed inside of a cap that is attached to a container; the cap may have an opening allowing a shaft, e.g., a probe or sipper shaft, to be inserted into the cap and into the plunger head. The shaft may be used to push the plunger head free of the cap, and then to reciprocate the plunger head within the container to mix the content. Withdrawal of the shaft may then cause the plunger head to disengage from the shaft and remain in the container.

Abstract: A system may include a flow cell through which a plurality of reagents may be pumped during a genetic sequencing operation, an effluent line that, in operation, is to conduct a used reagent, a used reagent valve to receive the used reagent from the effluent line and controllable to select one of a plurality of disposal paths for the used reagent, and control circuitry coupled to the used reagent valve that, in operation, is to control the used reagent valve to select a desired one of the disposal paths depending upon which reagent is being pumped though the flow cell.

Abstract: An analysis system includes a fluidic system includes a number of components that are interconnected to form a fluidic system having a plurality of flow paths. An example method of pressure testing the fluidic system includes (a) selecting a flow path from the plurality of flow paths through a flow cell in accordance with a prescribed test protocol; (b) actuating a pump to pressurize a fluid in the selected flow path; (c) generating pressure data representative of the pressure in the selected flow path; and (d) processing the pressure data to determine whether the selected flow path maintains pressure in a desired manner.

Abstract: A method includes, under control of control circuitry implementing a mixing protocol, aspirating reagents from multiple different reagent reservoirs into a cache channel. Designated amounts of the reagents are automatically aspirated from the corresponding reagent reservoirs by corresponding sippers based on the mixing protocol implemented by the control circuitry. The method also includes discharging the reagents from the cache channel into a mixing reservoir, and mixing the reagents within the mixing reservoir to form a reagent mixture.

Abstract: A system utilizes a flow cell for holding an analyte of interest for examination, such as a genetic material to be imaged for sequencing. Liquids, such as reagents and washing fluids are introduced into the flow cell during operations. A degasser removes gasses from at least some of the liquids before introduction into the flow cell. The liquids may be resident in the flow cell during detection operations, such as imaging. At least one fluid may be moved bidirectionally into and from the flow cell, such as for reuse. Another fluid may be moved unidirectionally through the flow cell to remove bubbles that may be present in the system.

Abstract: A system includes a reagent selector valve controllable to select a reagent flow path from a plurality of reagent flow paths, and a pump coupled to the reagent flow path to draw a liquid through the reagent flow path in accordance with a prescribed test protocol. The system includes a discharge flow path to expel the drawn liquid, and a flow meter to measure liquid displaced by the pump and that outputs data representative of the measured flow. The system also includes a processor to access the data and to determine a volume of the liquid displaced by the pump.

Abstract: Detection apparatus includes a microfluorometer having an objective, an excitation radiation source, and a detector. The detection apparatus also includes a fluidic system for delivering reagents from a reagent cartridge to a flow cell. The fluidic system includes a manifold body having a plurality of fluidic channels configured for fluid communication between the reagent cartridge and the flow cell. The fluidic system also includes a plurality of reagent sippers. The fluidic system also includes a valve configured to mediate fluid between reagent reservoirs and the flow cell. The detection apparatus also includes a flow cell latch clamp module having a clamp cover for holding the flow cell. The objective is configured to direct excitation radiation from the radiation source to the flow cell and to direct emission from the flow cell to the detector. The microfluorometer is movable to acquire wide-field images of different areas of the flow cell.

Abstract: A mounting alignment apparatus may include a rail assembly including a first pair of alignment surfaces. The mounting alignment apparatus may also include a slide assembly including a second pair of alignment surfaces. The first and second pairs of alignment surfaces may define an interacting capturing geometry therebetween, permitting sliding movement of the slide assembly relative to the rail assembly along a first axis, and restricting movement of the slide assembly relative to the rail assembly about an axis other than the first axis. The mounting alignment apparatus may also include a tension adjustment assembly coupled between the rail assembly and the slide assembly for positioning the slide assembly relative to the rail assembly along the first axis.

Abstract: A microfluid ejection system includes a fluid path having proximate and distal ends, the proximate end having an inlet to receive fluid from a fluid supply, a vacuum chamber for suctioning of air from both the proximate and distal ends of the fluid path, and a pre-ejection chamber disposed in the fluid path between a proximate end and a distal end. The pre-ejection chamber includes a ceiling inclined upward toward each of the proximate and distal ends from a low point to direct air toward either the proximate or distal end for suctioning from the pre-ejection chamber. The microfluid system further includes a first and second air chambers disposed respectively at the proximate and distal ends of the fluid path to receive and direct air to the vacuum chamber.

Abstract: A method for producing a coated optical fiber may include drawing an optical fiber from a draw furnace along a first vertical pathway. The optical fiber may then be routed through at least one fluid bearing thereby redirecting the optical fiber along a second vertical pathway. Thereafter, a thermoplastic coating may be applied to the optical fiber with a thermoplastic coating system. The optical fiber may then be wound onto a fiber storage spool with a fiber take-up system. The fiber take-up system may be space apart from the thermoplastic coating system such that the thermoplastic coating may be cooled before the optical fiber is wound onto the fiber storage spool.

Abstract: A microfluid ejection system includes a fluid path having proximate and distal ends, the proximate end having an inlet to receive fluid from a fluid supply, a vacuum chamber for suctioning of air from both the proximate and distal ends of the fluid path, and a pre-ejection chamber disposed in the fluid path between a proximate end and a distal end. The pre-ejection chamber includes a ceiling inclined upward toward each of the proximate and distal ends from a low point to direct air toward either the proximate or distal end for suctioning from the pre-ejection chamber. The microfluid system further includes a first and second air chambers disposed respectively at the proximate and distal ends of the fluid path to receive and direct air to the vacuum chamber.