Patents by Inventor Bradley R. Snedecor
Bradley R. Snedecor has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230279583Abstract: The presently disclosed subject matter relates to “Randomized Configuration Targeted Integration” (also referred to herein as “Randomized Chain Targeted Integration”) (RCTI) strategies for the generation and identification of host cells capable of expressing recombinant proteins, e.g., monoclonal antibodies, as well as compositions derived from the same, e.g., bispecific antibodies, and other complex format proteins, e.g., membrane protein complexes and other difficult to express molecules.Type: ApplicationFiled: April 3, 2023Publication date: September 7, 2023Applicant: GENENTECH, INC.Inventors: Shahram Misaghi, Bradley R. Snedecor
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Patent number: 11634836Abstract: The presently disclosed subject matter relates to “Randomized Configuration Targeted Integration” (also referred to herein as “Randomized Chain Targeted Integration”) (RCTI) strategies for the generation and identification of host cells capable of expressing recombinant proteins, e.g., monoclonal antibodies, as well as compositions derived from the same, e.g., bispecific antibodies, and other complex format proteins, e.g., membrane protein complexes and other difficult to express molecules.Type: GrantFiled: December 27, 2021Date of Patent: April 25, 2023Assignee: Genentech, Inc.Inventors: Shahram Misaghi, Bradley R. Snedecor
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Publication number: 20230069966Abstract: The present invention contemplates a method of producing a recombinant protein comprising (a) fermenting a prokaryotic host cell wherein said prokaryotic host cell has been transformed with a nucleic acid encoding said recombinant protein, and (b) harvesting said recombinant protein under conditions where dO2 levels are greater than 0%, and (c) purifying said recombinant protein to a filtered bulk, wherein said filtered bulk does not contain detectable DHNA-recombinant protein adduct, as measured by an IEC assay at 310 nm.Type: ApplicationFiled: July 13, 2022Publication date: March 9, 2023Applicant: Genentech, Inc.Inventors: Michael W. LAIRD, Richard ST. JOHN, Jane V. GUNSON, Kimberly KALEAS, Deepa NADARAJAH, Rachel L.E. ADAMS, Bradley R. SNEDECOR
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Publication number: 20220119985Abstract: The presently disclosed subject matter relates to “Randomized Configuration Targeted Integration” (also referred to herein as “Randomized Chain Targeted Integration”) (RCTI) strategies for the generation and identification of host cells capable of expressing recombinant proteins, e.g., monoclonal antibodies, as well as compositions derived from the same, e.g., bispecific antibodies, and other complex format proteins, e.g., membrane protein complexes and other difficult to express molecules.Type: ApplicationFiled: December 27, 2021Publication date: April 21, 2022Applicant: GENENTECH, INC.Inventors: Shahram MISAGHI, Bradley R. SNEDECOR
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Publication number: 20210171997Abstract: The present invention contemplates a method of producing a recombinant protein comprising (a) fermenting a prokaryotic host cell wherein said prokaryotic host cell has been transformed with a nucleic acid encoding said recombinant protein, and (b) harvesting said recombinant protein under conditions where dO2 levels are greater than 0%, and (c) purifying said recombinant protein to a filtered bulk, wherein said filtered bulk does not contain detectable DHNA-recombinant protein adduct, as measured by an IEC assay at 310 nm.Type: ApplicationFiled: October 9, 2020Publication date: June 10, 2021Applicant: Genentech, Inc.Inventors: Michael W. LAIRD, Richard ST. JOHN, Jane V. GUNSON, Kimberly KALEAS, Deepa NADARAJAH, Rachel L.E. ADAMS, Bradley R. SNEDECOR
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Publication number: 20210002669Abstract: The presently disclosed subject matter relates to targeted integration (TI) host cells suitable for the expression of recombinant proteins, as well as methods of producing and using said TI host cells.Type: ApplicationFiled: December 21, 2018Publication date: January 7, 2021Applicant: GENENTECH, INC.Inventors: Chi Kin Domingos Ng, Yongping Guo Crawford, Amy Shen, Meixia Zhou, Bradley R. Snedecor, Shahram Misaghi, Albert Eric Gao
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Publication number: 20200199639Abstract: The present invention contemplates a method of producing a recombinant protein comprising (a) fermenting a prokaryotic host cell wherein said prokaryotic host cell has been transformed with a nucleic acid encoding said recombinant protein, and (b) harvesting said recombinant protein under conditions where dO2 levels are greater than 0%, and (c) purifying said recombinant protein to a filtered bulk, wherein said filtered bulk does not contain detectable DHNA-recombinant protein adduct, as measured by an IEC assay at 310 nm.Type: ApplicationFiled: February 7, 2019Publication date: June 25, 2020Applicant: Genentech, Inc.Inventors: Michael W. LAIRD, Richard ST. JOHN, Jane V. GUNSON, Kimberly KALEAS, Deepa NADARAJAH, Rachel L.E. ADAMS, Bradley R. SNEDECOR
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Publication number: 20180291411Abstract: The present invention contemplates a method of producing a recombinant protein comprising (a) fermenting a prokaryotic host cell wherein said prokaryotic host cell has been transformed with a nucleic acid encoding said recombinant protein, and (b) harvesting said recombinant protein under conditions where dO2 levels are greater than 0%, and (c) purifying said recombinant protein to a filtered bulk, wherein said filtered bulk does not contain detectable DHNA-recombinant protein adduct, as measured by an IEC assay at 310 nm.Type: ApplicationFiled: February 28, 2018Publication date: October 11, 2018Inventors: Michael W. LAIRD, Richard ST. JOHN, Jane V. GUNSON, Kimberly KALEAS, Deepa NADARAJAH, Rachel L.E. ADAMS, Bradley R. SNEDECOR
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Patent number: 9765379Abstract: The present invention contemplates methods of producing a recombinant protein comprising fermenting a prokaryotic host cell wherein said prokaryotic host cell has been transformed with a nucleic acid encoding said recombinant protein, harvesting said recombinant protein under conditions where dO2 levels are greater than 0%, purifying said recombinant protein to a filtered bulk, wherein said filtered bulk does not contain detectable DHNA-recombinant protein adduct, as measured by an IEC assay at 310 nm.Type: GrantFiled: September 26, 2014Date of Patent: September 19, 2017Assignee: Genentech, Inc.Inventors: Michael W. Laird, Richard St. John, Jane V. Gunson, Kimberly Kaleas, Deepa Nadarajah, Rachel L E Adams, Bradley R. Snedecor
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Publication number: 20160130624Abstract: The present invention contemplates a method of producing a recombinant protein comprising (a) fermenting a prokaryotic host cell wherein said prokaryotic host cell has been transformed with a nucleic acid encoding said recombinant protein, and (b) harvesting said recombinant protein under conditions where dO2 levels are greater than 0%, and (c) purifying said recombinant protein to a filtered bulk, wherein said filtered bulk does not contain detectable DHNA-recombinant protein adduct, as measured by an IEC assay at 310 nm.Type: ApplicationFiled: June 9, 2015Publication date: May 12, 2016Applicant: Genentech, Inc.Inventors: Michael W. LAIRD, Richard ST. JOHN, Jane V. GUNSON, Kimberly KALEAS, Deepa NADARAJAH, Rachel L.E. ADAMS, Bradley R. SNEDECOR
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Publication number: 20150225760Abstract: The present invention contemplates methods of producing a recombinant protein comprising fermenting a prokaryotic host cell wherein said prokaryotic host cell has been transformed with a nucleic acid encoding said recombinant protein, harvesting said recombinant protein under conditions where dO2 levels are greater than 0%, purifying said recombinant protein to a filtered bulk, wherein said filtered bulk does not contain detectable DHNA-recombinant protein adduct, as measured by an IEC assay at 310 nm.Type: ApplicationFiled: September 26, 2014Publication date: August 13, 2015Applicant: Genentech, Inc.Inventors: Michael W. LAIRD, Richard ST. JOHN, Jane V. GUNSON, Kimberly KALEAS, Deepa NADARAJAH, Rachel L E ADAMS, Bradley R. SNEDECOR
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Publication number: 20090208500Abstract: The invention provides methods for controlling fucosylation levels and improving ADCC activity in antibodies.Type: ApplicationFiled: June 5, 2006Publication date: August 20, 2009Applicant: Genentech, Inc.Inventors: John C. Joly, Henry B. Lowman, Domingos Ng, Amy Y. Shen, Bradley R. Snedecor
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Publication number: 20080166759Abstract: Polypeptides that are cleared from the kidney and do not contain in their original form a Fc region of an IgG are altered so as to comprise a salvage receptor binding epitope of an Fc region of an IgG and thereby have increased circulatory half-life.Type: ApplicationFiled: July 30, 2007Publication date: July 10, 2008Applicant: Genentech, Inc.Inventors: Leonard G. Presta, Bradley R. Snedecor
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Patent number: 6998253Abstract: Polypeptides that are cleared from the kidney and do not contain in their original form a Fc region of an IgG are altered so as to comprise a salvage receptor binding epitope of an Fc region of an IgG and thereby have increased circulatory half-life.Type: GrantFiled: July 31, 2000Date of Patent: February 14, 2006Assignee: Genentech, Inc.Inventors: Leonard G. Presta, Bradley R. Snedecor
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Patent number: 6716602Abstract: The invention provides a method for increasing product yield of a polypeptide of interest produced by recombinant host cells, where expression of the polypeptide by the recombinant host cells is regulated by an inducible system. More specifically, the method involves culturing the recombinant host cells under conditions of high metabolic and growth rate and then reducing the metabolic rate of the recombinant host cells at the time of induction of polypeptide expression. In particular, the invention provides a method of increasing product yield of an antibody, growth factor, or protease produced by a recombinant E. coli host cell regulated by an inducible system.Type: GrantFiled: November 1, 2001Date of Patent: April 6, 2004Assignee: Genentech, Inc.Inventors: Dana Andersen, John Joly, Bradley R. Snedecor
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Publication number: 20020164700Abstract: The invention provides a method for increasing product yield of a polypeptide of interest produced by recombinant host cells, where expression of the polypeptide by the recombinant host cells is regulated by an inducible system. More specifically, the method involves culturing the recombinant host cells under conditions of high metabolic and growth rate and then reducing the metabolic rate of the recombinant host cells at the time of induction of polypeptide expression. In particular, the invention provides a method of increasing product yield of an antibody, growth factor, or protease produced by a recombinant E.Type: ApplicationFiled: November 1, 2001Publication date: November 7, 2002Applicant: Genentech, Inc.Inventors: Dana Andersen, John Joly, Bradley R. Snedecor
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Patent number: 6121022Abstract: Polypeptides that are cleared from the kidney and do not contain in their original form a Fc region of an IgG are altered so as to comprise a salvage receptor binding epitope of an Fc region of an IgG and thereby have increased circulatory half-life.Type: GrantFiled: April 14, 1995Date of Patent: September 19, 2000Assignee: Genentech, Inc.Inventors: Leonard G. Presta, Bradley R. Snedecor
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Patent number: 6096871Abstract: Polypeptides that are cleared from the kidney and do not contain in their original form a Fc region of an IgG are altered so as to comprise a salvage receptor binding epitope of an Fc region of an IgG and thereby have increased circulatory half-life.Type: GrantFiled: April 14, 1995Date of Patent: August 1, 2000Assignee: Genentech, Inc.Inventors: Leonard G. Presta, Bradley R. Snedecor
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Patent number: 5869046Abstract: Polypeptides that are cleared from the kidney and do not contain in their original form a Fc region of an IgG are altered so as to comprise a salvage receptor binding epitope of an Fc region of an IgG and thereby have increased circulatory half-life.Type: GrantFiled: April 14, 1995Date of Patent: February 9, 1999Assignee: Genentech, Inc.Inventors: Leonard G. Presta, Bradley R. Snedecor
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Patent number: 5747035Abstract: Polypeptides that are cleared from the kidney and do not contain in their original form a Fc region of an IgG are altered so as to comprise a salvage receptor binding epitope of an Fc region of an IgG and thereby have increased circulatory half-life. Methods are described herein which utilize these polypeptides in treating disorders involving the LFA-1 receptor. In one of the described methods of treatment, the polypeptide includes the amino acid sequence PKNSSMISNTP (SEQ ID NO:3) and may also include the sequence selected from the group consisting of HQNLSDGK (SEQ ID NO: 1), HQNISDGK (SEQ ID NO:2), HQSLGTQ (SEQ ID NO:11) and VISSHLGQ (SEQ ID NO:31).Type: GrantFiled: April 14, 1995Date of Patent: May 5, 1998Assignee: Genentech, Inc.Inventors: Leonard G. Presta, Bradley R. Snedecor