Abstract: Disclosed herein are protease-based biological circuits for use in the diagnosis and treatment of disease and disorders characterized by aberrant protease signaling. An exemplary method of treating disease in a subject includes administering to the subject a plurality of therapeutic agent loaded-liposomes, wherein each of the liposomes comprises a different density of peptides surrounding the core and a different dose of the therapeutic agent, wherein the peptides comprise a cleavage site for a protease of interest, and wherein cleavage of the peptides open the liposome and release the therapeutic agent from within the liposome.

Abstract: Disclosed herein are bacterial protease-activated prodrug compositions and methods of their use for the treatment and prevention of bacterial infection. Also disclosed are methods of reducing or eliminating defiant bacterial populations. An exemplary prodrug composition includes a cationic antimicrobial peptide conjugated to an anionic peptide with a protease cleavable linker substrate, wherein the antimicrobial peptide is inactive while it is in conjugation with the anionic peptide. Upon cleavage by the protease, the cationic AMP is released from the anionic peptide and can act upon bacteria. The protease is specific to the bacteria that are present in the sample or the subject, creating an auto-titrating mechanism wherein the AMP is released from the peptide only when there is bacteria present.

Abstract: The disclosure provides methods and compositions to digitally profile biological activity by detecting the activity of at least two biomarkers using protease activity sensors or biocomparators.

Abstract: A computing system for scalable computing on commodity hardware is provided. The computing system includes a first computing device communicatively connected to a second computing device. The first computing device includes a processor, a physical computer-readable medium, and program instructions stored on the physical computer-readable medium and executable by the processor to perform functions. The functions include determining a first task associated with the second computing device and a second task associated with the second computing device are to be executed, assigning execution of the first task and the second task to the processor of the first computing device, generating an aggregated message that includes (i) a first message including an indication corresponding to the execution of the first task and (ii) a second message including an indication corresponding to the execution of the second task, and sending the aggregated message to the second computing device.