Abstract: Provided are methods of determining bound and unbound regions in nucleic acid molecules. In certain aspects, the methods include forming adducts in a double-stranded nucleic acid molecule that mark the locations of unbound regions in the double-stranded nucleic acid, and detecting the locations of the adducts in the double-stranded nucleic acid using a nanopore. Bound regions of the double-stranded nucleic acid molecule are determined based on the absence of adducts. In certain aspects, the double-stranded nucleic acid molecule is genomic DNA and the bound regions are nucleosome positions. As such, encompassed by the methods are methods of determining nucleosome positions in genomic DNA. Systems and kits that find use, e.g., in practicing the methods of the present disclosure are also provided.

Abstract: A low-weight rupture/ballistics resistant wall structure is provided. Such a structure includes a multi-layer composite of at least two triaxial quasi three-dimensional woven fabrics, each coated with a specific depth of a polyurea material. Additionally, each fabric layer may be impregnated with an epoxy material to retain the triaxial configuration during utilization. Such a composite is adhered to a steel or other type of metallic base to provide improved strength to the base structure without significantly increasing the overall weight thereof. In such a manner, the inventive composite mitigates against undesirable rupture or other compromise of the overall metal structure during a destructive event and even permits reliable resealing of any created opening therein as a result of such an occurrence. The process of manufacturing the composite as well as the method of providing increased ballistic protection are also encompassed within this invention.