Patents by Inventor Brenda Vonk

Brenda Vonk has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 11149268
    Abstract: The present invention is based on the advantageous use of single-stranded oligonucleotides in the in vivo (within a cell) assembly of double-stranded oligonucleotides into a single double-stranded nucleic acid construct. The present invention relates to the use of at least a first and a second single-stranded oligonucleotide in the assembly within a cell of at least two double-stranded nucleic acid molecules into a single double-stranded nucleic acid construct of pre-determined sequence, wherein the first and second single-stranded oligonucleotide are essentially complementary to each other.
    Type: Grant
    Filed: December 15, 2016
    Date of Patent: October 19, 2021
    Assignee: DSM IP ASSETS B.V.
    Inventors: Johannes Andries Roubos, René Verwaal, Bianca Elisabeth Maria Gielesen, Brenda Vonk
  • Patent number: 11118193
    Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR-CAS system for a yeast host cell.
    Type: Grant
    Filed: February 3, 2020
    Date of Patent: September 14, 2021
    Assignee: DSM IP ASSESTS B.V.
    Inventors: René Verwaal, Bernard Meijrink, Nathalie Wiessenhaan, Brenda Vonk, Johannes Andries Roubos
  • Publication number: 20210071174
    Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR transient expression construct (CTEC) for a genome editing system.
    Type: Application
    Filed: May 6, 2019
    Publication date: March 11, 2021
    Inventors: Johannes Andries ROUBOS, Rene VERWAAL, Brenda VONK
  • Publication number: 20200199599
    Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a guide-RNA expression system for a eukaryotic host cell.
    Type: Application
    Filed: July 17, 2017
    Publication date: June 25, 2020
    Inventors: Eric Mosher YOUNG, Amar GHODASARA, René VERWAAL, Johannes Andries ROUBOS, Bianca Elisabeth Maria GIELESEN, Brenda VONK, Alec A.K. NIELSEN, Christopher Ashby VOIGT
  • Publication number: 20200172934
    Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR-CAS system for a yeast host cell.
    Type: Application
    Filed: February 3, 2020
    Publication date: June 4, 2020
    Inventors: René VERWAAL, Bernard MEIJRINK, Nathalie WIESSENHAAN, Brenda VONK, Johannes Andries ROUBOS
  • Patent number: 10619170
    Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR-CAS system for a yeast host cell.
    Type: Grant
    Filed: January 6, 2016
    Date of Patent: April 14, 2020
    Assignee: DSM IP ASSETS B.V.
    Inventors: René Verwaal, Bernard Meijrink, Nathalie Wiessenhaan, Brenda Vonk, Johannes Andries Roubos
  • Publication number: 20200032252
    Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a self-guiding integration construct for a genome editing system.
    Type: Application
    Filed: April 4, 2018
    Publication date: January 30, 2020
    Inventors: Johannes Andries ROUBOS, René VERWAAL, Bianca Elisabeth Maria GIELESEN, Brenda VONK
  • Publication number: 20190185843
    Abstract: The present invention is based on the advantageous use of single-stranded oligonucleotides in the in vivo (within a cell) assembly of double-stranded oligonucleotides into a single double-stranded nucleic acid construct. The present invention relates to the use of at least a first and a second single-stranded oligonucleotide in the assembly within a cell of at least two double-stranded nucleic acid molecules into a single double-stranded nucleic acid construct of pre-determined sequence, wherein the first and second single-stranded oligonucleotide are essentially complementary to each other.
    Type: Application
    Filed: December 15, 2016
    Publication date: June 20, 2019
    Inventors: Johannes Andries ROUBOS, René VERWAAL, Blance Elisabeth Maria GIELESEN, Brenda VONK
  • Publication number: 20180010151
    Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR-CAS system for a yeast host cell.
    Type: Application
    Filed: January 6, 2016
    Publication date: January 11, 2018
    Inventors: René VERWAAL, Bernard MEIJRINK, Nathalie WIESSENHAAN, Brenda VONK, Johannes Andries ROUBOS
  • Publication number: 20150197760
    Abstract: The present invention relates to isolated Rasamsonia promoter DNA sequences, to DNA constructs, vectors, and host cells comprising these promoters in operative association with coding sequences. The present invention also relates to methods for expressing a gene and/or producing a biological compound using the new promoters isolated. The present invention also relates to methods for altering the transcription level and/or regulation of an endogenous gene using the new promoter of the invention.
    Type: Application
    Filed: June 17, 2013
    Publication date: July 16, 2015
    Inventors: Alrik Pieter Los, Wilbert Herman Marie Heijne, Herman Jan Pel, Robbertus Antonius Damveld, Brenda Vonk
  • Patent number: 8802415
    Abstract: The invention relates to a Talaromyces transformant comprising one or more recombinant gene, capable of producing cellulase in the absence of cellulase inducer in a glucose medium, having a cellulase activity of 2 WSU/ml or more, in 16 times or more diluted supernatant or broth.
    Type: Grant
    Filed: November 4, 2010
    Date of Patent: August 12, 2014
    Assignee: DSM IP Assets B.V.
    Inventors: Alrik Pieter Los, Brenda Vonk, Marco Alexander Van Den Berg, Robbertus Antonius Damveld, Cornelis Maria Jacobus Sagt, Adrianus Wilhelmus Hermanus Vollebregt, Margot Elisabeth Francoise Schooneveld-Bergmans
  • Publication number: 20120276567
    Abstract: The invention relates to a Talaromyces transformant comprising one or more recombinant gene, capable of producing cellulase in the absence of cellulase inducer in a glucose medium, having a cellulase activity of 2 WSU/ml or more, in 16 times or more diluted supernatant or broth.
    Type: Application
    Filed: November 4, 2010
    Publication date: November 1, 2012
    Applicant: DSM IP ASSETS B.V.
    Inventors: Alrik Pieter Los, Brenda Vonk, Marco Alexander Berg Van Den, Robbertus Antonius Damveld, Cornelis Maria Jacobus Sagt, Adrianus Wilhelmus Hermanus Vollebregt, Margot Elisabeth Francoise Schooneveld-Bergmans
  • Publication number: 20090209007
    Abstract: The invention relates to a promoter DNA sequence highly suited in an improved expression cloning method for isolation of DNA sequences comprising a DNA sequence encoding a protein of interest in a host cell and to the improved expression cloning method wherein use is made of this promoter. The isolated DNA sequences are useful in processes for producing a protein of interest.
    Type: Application
    Filed: March 20, 2006
    Publication date: August 20, 2009
    Inventors: Roelf Bernhard Meima, Thibaut Jose Wenzel, Cornelis Maria Jacobus Sagt, Johannes Hendrik Winde, Oscar Paul Kuipers, Rutger Jan Van Rooijen, Marjolein Cornelia Dekker, Brenda Vonk
  • Publication number: 20080070277
    Abstract: The present invention relates to novel functional amdS genes from A. niger that can be used as dominant and bi-directional selection marker gene in the transformation of organisms. The present invention further relates to the production of a compound of interest in a fungal host cell transformed with the amdS genes of the invention. Preferred fungal host cells are filamentous fungal cells. The amdS genes of the invention provide means for identification of functional homologues in other Aspergillus species.
    Type: Application
    Filed: October 17, 2005
    Publication date: March 20, 2008
    Inventors: Cornelis Sagt, Johannes Winde, Thibaut Wenzel, Brenda Vonk