Patents by Inventor Brendan J. Tarrier

Brendan J. Tarrier has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20210292828
    Abstract: The present invention concerns preparation of DNA molecules, such as a library, using a stem-loop oligonucleotide. In particular embodiments, the invention employs a single reaction mixture and conditions. In particular, at least part of the inverted palindrome is removed during the preparation of the molecules to facilitate amplification of the molecules. Thus, in specific embodiments, the DNA molecules are suitable for amplification and are not hindered by the presence of the palindrome.
    Type: Application
    Filed: May 21, 2021
    Publication date: September 23, 2021
    Inventors: Vladimir L. MAKAROV, Emmanuel KAMBEROV, Brendan J. TARRIER
  • Patent number: 11072823
    Abstract: The present invention concerns preparation of DNA molecules, such as a library, using a stem-loop oligonucleotide. In particular embodiments, the invention employs a single reaction mixture and conditions. In particular, at least part of the inverted palindrome is removed during the preparation of the molecules to facilitate amplification of the molecules. Thus, in specific embodiments, the DNA molecules are suitable for amplification and are not hindered by the presence of the palindrome.
    Type: Grant
    Filed: December 21, 2018
    Date of Patent: July 27, 2021
    Assignee: Takara Bio USA, Inc.
    Inventors: Vladimir L. Makarov, Emmanuel Kamberov, Brendan J. Tarrier
  • Publication number: 20190119741
    Abstract: The present invention concerns preparation of DNA molecules, such as a library, using a stem-loop oligonucleotide. In particular embodiments, the invention employs a single reaction mixture and conditions. In particular, at least part of the inverted palindrome is removed during the preparation of the molecules to facilitate amplification of the molecules. Thus, in specific embodiments, the DNA molecules are suitable for amplification and are not hindered by the presence of the palindrome.
    Type: Application
    Filed: December 21, 2018
    Publication date: April 25, 2019
    Inventors: Vladimir L. MAKAROV, Emmanuel KAMBEROV, Brendan J. TARRIER
  • Patent number: 10208337
    Abstract: The present invention concerns preparation of DNA molecules, such as a library, using a stem-loop oligonucleotide. In particular embodiments, the invention employs a single reaction mixture and conditions. In particular, at least part of the inverted palindrome is removed during the preparation of the molecules to facilitate amplification of the molecules. Thus, in specific embodiments, the DNA molecules are suitable for amplification and are not hindered by the presence of the palindrome.
    Type: Grant
    Filed: August 10, 2017
    Date of Patent: February 19, 2019
    Assignee: TAKARA BIO USA, INC.
    Inventors: Vladimir L. Makarov, Emmanuel Kamberov, Brendan J. Tarrier
  • Patent number: 10196686
    Abstract: The present invention concerns preparation of DNA molecules, such as a library, using a stem-loop oligonucleotide. In particular embodiments, the invention employs a single reaction mixture and conditions. In particular, at least part of the inverted palindrome is removed during the preparation of the molecules to facilitate amplification of the molecules. Thus, in specific embodiments, the DNA molecules are suitable for amplification and are not hindered by the presence of the palindrome.
    Type: Grant
    Filed: February 13, 2017
    Date of Patent: February 5, 2019
    Assignee: TAKARA BIO USA, INC.
    Inventors: Vladimir L. Makarov, Emmanuel Kamberov, Brendan J. Tarrier
  • Publication number: 20180030527
    Abstract: The present invention regards a variety of methods and compositions for obtaining epigenetic information, such as DNA methylation patterns, through the preparation, amplification and analysis of Methylome libraries.
    Type: Application
    Filed: June 12, 2017
    Publication date: February 1, 2018
    Inventors: Vladimir L. MAKAROV, Emmanuel KAMBEROV, Tong SUN, Jonathan H. PINTER, Brendan J. TARRIER, Eric E. BRUENING, Takao KURIHARA, Tim TESMER, Joseph M'Mwirichia
  • Publication number: 20170342475
    Abstract: The present invention concerns preparation of DNA molecules, such as a library, using a stem-loop oligonucleotide. In particular embodiments, the invention employs a single reaction mixture and conditions. In particular, at least part of the inverted palindrome is removed during the preparation of the molecules to facilitate amplification of the molecules. Thus, in specific embodiments, the DNA molecules are suitable for amplification and are not hindered by the presence of the palindrome.
    Type: Application
    Filed: August 10, 2017
    Publication date: November 30, 2017
    Inventors: Vladimir L. MAKAROV, Emmanuel KAMBEROV, Brendan J. TARRIER
  • Publication number: 20170314069
    Abstract: The present invention concerns preparation of DNA molecules, such as a library, using a stem-loop oligonucleotide. In particular embodiments, the invention employs a single reaction mixture and conditions. In particular, at least part of the inverted palindrome is removed during the preparation of the molecules to facilitate amplification of the molecules. Thus, in specific embodiments, the DNA molecules are suitable for amplification and are not hindered by the presence of the palindrome.
    Type: Application
    Filed: February 13, 2017
    Publication date: November 2, 2017
    Applicant: Rubicon Genomics, Inc.
    Inventors: Vladimir L. MAKAROV, Emmanuel KAMBEROV, Brendan J. TARRIER
  • Patent number: 9708652
    Abstract: The present invention regards a variety of methods and compositions for obtaining epigenetic information, such as DNA methylation patterns, through the preparation, amplification and analysis of Methylome libraries.
    Type: Grant
    Filed: April 9, 2013
    Date of Patent: July 18, 2017
    Assignee: RUBICON GENOMICS, INC.
    Inventors: Vladimir L. Makarov, Emmanuel Kamberov, Tong Sun, Jonathan H. Pinter, Brendan J. Tarrier, Eric E. Bruening, Takao Kurihara, Tim Tesmer, Joseph M'Mwirichia
  • Patent number: 9598727
    Abstract: The present invention is directed generally to the use of stem-loop oligonucleotides comprising an inverted repeat, a loop, and at least one degradable site or site capable of becoming a degradable site in the preparation of DNA molecules, such as a library. In particular embodiments, the invention employs a single reaction mixture and conditions. In particular, at least part of the inverted palindrome is removed during the preparation of the molecules to facilitate amplification of the molecules. Thus, in specific embodiments, the DNA molecules are suitable for amplification and are not hindered by the presence of the palindrome.
    Type: Grant
    Filed: April 11, 2014
    Date of Patent: March 21, 2017
    Assignee: Rubicon Genomics, Inc.
    Inventors: Vladimir L. Makarov, Emmanuel Kamberov, Brendan J. Tarrier
  • Publication number: 20150079637
    Abstract: The present invention concerns preparation of DNA molecules, such as a library, using a stem-loop oligonucleotide. In particular embodiments, the invention employs a single reaction mixture and conditions. In particular, at least part of the inverted palindrome is removed during the preparation of the molecules to facilitate amplification of the molecules. Thus, in specific embodiments, the DNA molecules are suitable for amplification and are not hindered by the presence of the palindrome.
    Type: Application
    Filed: April 11, 2014
    Publication date: March 19, 2015
    Applicant: RUBICON GENOMICS, INC.
    Inventors: Vladimir L. MAKAROV, Emmanuel KAMBEROV, Brendan J. TARRIER
  • Patent number: 8778610
    Abstract: The present invention concerns isolation, library preparation and selective amplification from a compositionally heterogeneous pool of DNA fragments of a fraction of molecules, such as those originating from promoter CpG islands and characterized by a high GC content. In particular, the process utilizes a heat-induced segregation of DNA molecules into GC-poor, single-stranded molecule fractions and GC-rich, double-stranded molecule fractions, with subsequent enzymatic conversion of the GC-rich, double-stranded DNA molecules into a library, and, optionally, amplification. In specific embodiments, the isolation process is used to generate promoter-enriched genomic and methylome libraries for research and diagnostic applications, for example.
    Type: Grant
    Filed: February 27, 2013
    Date of Patent: July 15, 2014
    Assignee: Rubicon Genomics, Inc.
    Inventors: Vladimir L. Makarov, Emmanuel Kamberov, Brendan J. Tarrier
  • Patent number: 8728737
    Abstract: The present invention concerns preparation of DNA molecules, such as a library, using a stem-loop oligonucleotide. In particular embodiments, the invention employs a single reaction mixture and conditions. In particular, at least part of the inverted palindrome is removed during the preparation of the molecules to facilitate amplification of the molecules. Thus, in specific embodiments, the DNA molecules are suitable for amplification and are not hindered by the presence of the palindrome.
    Type: Grant
    Filed: February 13, 2013
    Date of Patent: May 20, 2014
    Assignee: Rubicon Genomics, Inc.
    Inventors: Vladimir L. Makarov, Emmanuel Kamberov, Brendan J. Tarrier
  • Publication number: 20130303407
    Abstract: The present invention concerns preparation of DNA molecules, such as a library, using a stem-loop oligonucleotide. In particular embodiments, the invention employs a single reaction mixture and conditions. In particular, at least part of the inverted palindrome is removed during the preparation of the molecules to facilitate amplification of the molecules. Thus, in specific embodiments, the DNA molecules are suitable for amplification and are not hindered by the presence of the palindrome.
    Type: Application
    Filed: February 13, 2013
    Publication date: November 14, 2013
    Inventors: Vladimir L. MAKAROV, Emmanuel KAMBEROV, Brendan J. TARRIER
  • Patent number: 8440404
    Abstract: The present invention regards a variety of methods and compositions for obtaining epigenetic information, such as DNA methylation patterns, through the preparation, amplification and analysis of Methylome libraries.
    Type: Grant
    Filed: March 3, 2005
    Date of Patent: May 14, 2013
    Assignee: Rubicon Genomics
    Inventors: Vladimir L. Makarov, Emmanuel Kamberov, Tong Sun, Jonathon H. Pinter, Brendan J. Tarrier, Eric E. Bruening, Takao Kurihara, Tim Tesmer, Joseph M'Mwirichia
  • Patent number: 8409804
    Abstract: The present invention concerns isolation, library preparation and selective amplification from a compositionally heterogeneous pool of DNA fragments of a fraction of molecules, such as those originating from promoter CpG islands and characterized by a high GC content. In particular, the process utilizes a heat-induced segregation of DNA molecules into GC-poor, single-stranded molecule fractions and GC-rich, double-stranded molecule fractions, with subsequent enzymatic conversion of the GC-rich, double-stranded DNA molecules into a library, and, optionally, amplification. In specific embodiments, the isolation process is used to generate promoter-enriched genomic and methylome libraries for research and diagnostic applications, for example.
    Type: Grant
    Filed: March 2, 2006
    Date of Patent: April 2, 2013
    Assignee: Rubicon Genomics, Inc.
    Inventors: Vladimir L. Makarov, Emmanuel Kamberov, Brendan J. Tarrier
  • Patent number: 8399199
    Abstract: The present invention concerns preparation of DNA molecules, such as a library, using a stem-loop oligonucleotide. In particular embodiments, the invention employs a single reaction mixture and conditions. In particular, at least part of the inverted palindrome is removed during the preparation of the molecules to facilitate amplification of the molecules. Thus, in specific embodiments, the DNA molecules are suitable for amplification and are not hindered by the presence of the palindrome.
    Type: Grant
    Filed: November 1, 2011
    Date of Patent: March 19, 2013
    Assignee: Rubicon Genomics
    Inventors: Vladimir L. Makarov, Emmanuel Kamberov, Brendan J. Tarrier
  • Publication number: 20120142060
    Abstract: The present invention concerns preparation of DNA molecules, such as a library, using a stem-loop oligonucleotide. In particular embodiments, the invention employs a single reaction mixture and conditions. In particular, at least part of the inverted palindrome is removed during the preparation of the molecules to facilitate amplification of the molecules. Thus, in specific embodiments, the DNA molecules are suitable for amplification and are not hindered by the presence of the palindrome.
    Type: Application
    Filed: November 1, 2011
    Publication date: June 7, 2012
    Applicant: Rubicon Genomics, Inc.
    Inventors: VLADIMIR L. MAKAROV, Emmanuel Kamberov, Brendan J. Tarrier
  • Patent number: 8071312
    Abstract: The present invention concerns preparation of DNA molecules, such as a library, using a stem-loop oligonucleotide. In particular embodiments, the invention employs a single reaction mixture and conditions. In particular, at least part of the inverted palindrome is removed during the preparation of the molecules to facilitate amplification of the molecules. Thus, in specific embodiments, the DNA molecules are suitable for amplification and are not hindered by the presence of the palindrome.
    Type: Grant
    Filed: September 28, 2010
    Date of Patent: December 6, 2011
    Assignee: Rubicon Genomics, Inc.
    Inventors: Vladimir L. Makarov, Emmanuel Kamberov, Brendan J. Tarrier
  • Publication number: 20110081685
    Abstract: The present invention concerns preparation of DNA molecules, such as a library, using a stem-loop oligonucleotide. In particular embodiments, the invention employs a single reaction mixture and conditions. In particular, at least part of the inverted palindrome is removed during the preparation of the molecules to facilitate amplification of the molecules. Thus, in specific embodiments, the DNA molecules are suitable for amplification and are not hindered by the presence of the palindrome.
    Type: Application
    Filed: September 28, 2010
    Publication date: April 7, 2011
    Applicant: Rubicon Genomics, Inc.
    Inventors: Vladimir L. Makarov, Emmanuel Kamberov, Brendan J. Tarrier