Abstract: A method, system and apparatus for applying and synchronizing filter information with a multimedia presentation, such as a movie provided in a video-on-demand context, to suppress objectionable content. In one example, filter information, which includes an indicia of a portion of the multimedia presentation including objectionable content and a type of suppression action, is provided on either a set-top-box or a video-on-demand server. A user selects a particular video-on-demand presentation, and the selection is transmitted to the set-top-box. Additionally, whether in a video-on-demand, DVD, or other environment it may be necessary to synchronize filter with the multimedia content so that the proper objectionable content is suppressed.

Abstract: A method, system and apparatus for applying and synchronizing filter information with a multimedia presentation, such as a movie provided in a video-on-demand context, to suppress objectionable content. In one example, filter information, which includes an indicia of a portion of the multimedia presentation including objectionable content and a type of suppression action, is provided on either a set-top-box or a video-on-demand server. A user selects a particular video-on-demand presentation, and the selection is transmitted to the set-top-box. Filtering may take place at the set-top-box side such that the complete video-on-demand movie is transmitted to the set-top box and then content suppression, such as skipping, muting, cropping, and blurring, takes place at the set-top-box. Alternatively, some or all suppression may take place at the server such that some or all of the objectionable content is not transmitted to the set-top-box.

Abstract: A media player configured with a first removable memory reader, such as a DVD drive, and a second removable memory reader, such as a flash memory reader, adapted to communicate with a removable memory containing filter data. The media player is configured to allow filtered playback of a multimedia presentation, such as a movie. Filtered playbabk causes certain portions of the multimedia presentation to be skipped, muted, blurred, cropped, or otherwise modified to eliminate or reduce potentially objectionable scenes, language, or other content. The second memory reader provides a convenient medium for the loading of filter information, whether data files, executable program code, or the like, to local memory of the media player to employ during filtered playback. Alternatively, the filters may be accessed from the removable storage media during playback rather than loading to local memory.

Abstract: A method, system and apparatus for applying and synchronizing filter information with a multimedia presentation, such as a movie provided in a video-on-demand context, to suppress objectionable content. In one example, filter information, which includes an indicia of a portion of the multimedia presentation including objectionable content and a type of suppression action, is provided on either a set-top-box or a video-on-demand server. A user selects a particular video-on-demand presentation, and the selection is transmitted to the set-top-box. Filtering may take place at the set-top-box side such that the complete video-on-demand movie is transmitted to the set-top box and then content suppression, such as skipping, muting, cropping, and blurring, takes place at the set-top-box. Alternatively, some or all suppression may take place at the server such that some or all of the objectionable content is not transmitted to the set-top-box.

Abstract: A media player configured with a first removable memory reader, such as a DVD drive, and a second removable memory reader, such as a flash memory reader, adapted to communicate with a removable memory containing filter data. The media player is configured to allow filtered playback of a multimedia presentation, such as a movie. Filtered playback causes certain portions of the multimedia presentation to be skipped, muted, blurred, cropped, or otherwise modified to eliminate or reduce potentially objectionable scenes, language, or other content. The second memory reader provides a convenient medium for the loading of filter information, whether data files, executable program code, or the like, to local memory of the media player to employ during filtered playback. Alternatively, the filters may be accessed from the removable storage media during playback rather than loading to local memory.

Abstract: A client server arrangement for downloading media content filters from a server device to a client device. The media content filters define portions of a separate audio visual presentation containing potentially objectionable subject matter. Depending on user selections, identified portions of the audio/visual presentation may be skipped and/or muted during play. In one particular implementation, the client device, e.g., a DVD player, is configured to initiate a connection with a server device. Upon successful connection, the server device transmits one or more media content filters to the client device. The client device may be configured to determine whether a particular media content filter is available, to facilitate deletion of some existing media content filters in order to secure adequate memory space, and to ensure that the media player has an active account, before initiating a connection with the server device.

Abstract: A media player configured with a first removable memory reader, such as a DVD drive, and a second removable memory reader, such as a flash memory reader, adapted to communicate with a removable memory containing filter data. The media player is configured to allow filtered playback of a multimedia presentation, such as a movie. Filtered playback causes certain portions of the multimedia presentation to be skipped, muted, blurred, cropped, or otherwise modified to eliminate or reduce potentially objectionable scenes, language, or other content. The second memory reader provides a convenient medium for the loading of filter information, whether data files, executable program code, or the like, to local memory of the media player to employ during filtered playback. Alternatively, the filters may be accessed from the removable storage media during playback rather than loading to local memory.

Abstract: A method, system and apparatus for applying and synchronizing filter information with a multimedia presentation, such as a movie provided in a video-on-demand context, to suppress objectionable content. In one example, filter information, which includes an indicia of a portion of the multimedia presentation including objectionable content and a type of suppression action, is provided on either a set-top-box or a video-on-demand server. A user selects a particular video-on-demand presentation, and the selection is transmitted to the set-top-box. Filtering may take place at the set-top-box side such that the complete video-on-demand movie is transmitted to the set-top box and then content suppression, such as skipping, muting, cropping, and blurring, takes place at the set-top-box. Alternatively, some or all suppression may take place at the server such that some or all of the objectionable content is not transmitted to the set-top-box.

Abstract: A method, system and apparatus for applying and synchronizing filter information with a multimedia presentation, such as a movie provided in a video-on-demand context, to suppress objectionable content. In one example, filter information, which includes an indicia of a portion of the multimedia presentation including objectionable content and a type of suppression action, is provided on either a set-top-box or a video-on-demand server. A user selects a particular video-on-demand presentation, and the selection is transmitted to the set-top-box. Filtering may take place at the set-top-box side such that the complete video-on-demand movie is transmitted to the set-top box and then content suppression, such as skipping, muting, cropping, and blurring, takes place at the set-top-box. Alternatively, some or all suppression may take place at the server such that some or all of the objectionable content is not transmitted to the set-top-box.

Abstract: A client server arrangement for downloading media content filters from a server device to a client device. The media content filters define portions of a separate audio visual presentation containing potentially objectionable subject matter. Depending on user selections, identified portions of the audio/visual presentation may be skipped and/or muted during play. In one particular implementation, the client device, e.g., a DVD player, is configured to initiate a connection with a server device. Upon successful connection, the server device transmits one or more media content filters to the client device. The client device may be configured to determine whether a particular media content filter is available, to facilitate deletion of some existing media content filters in order to secure adequate memory space, and to ensure that the media player has an active account, before initiating a connection with the server device.

Abstract: Provided are methods for protein engineering, such as engineering proteases or kinases. The methods may utilize yeast display and/or ER sequestration of proteins or substrates. In some aspects, TEV proteases with altered substrate specificity, potency, and/or efficiency are provided.

Abstract: A client server arrangement for downloading media content filters from a server device to a client device. The media content filters define portions of a separate audio visual presentation containing potentially objectionable subject matter. Depending on user selections, identified portions of the audio/visual presentation may be skipped and/or muted during play. In one particular implementation, the client device, e.g., a DVD player, is configured to initiate a connection with a server device. Upon successful connection, the server device transmits one or more media content filters to the client device. The client device may be configured to determine whether a particular media content filter is available, to facilitate deletion of some existing media content filters in order to secure adequate memory space, and to ensure that the media player has an active account, before initiating a connection with the server device.

Abstract: Provided are methods for protein engineering, such as engineering proteases or kinases. The methods may utilize yeast display and/or ER sequestration of proteins or substrates. In some aspects, TEV proteases with altered substrate specificity, potency, and/or efficiency are provided.

Abstract: A media player configured with a first removable memory reader, such as a DVD drive, and a second removable memory reader, such as a flash memory reader, adapted to communicate with a removable memory containing filter data. The media player is configured to allow filtered playback of a multimedia presentation, such as a movie. Filtered playback causes certain portions of the multimedia presentation to be skipped, muted, blurred, cropped, or otherwise modified to eliminate or reduce potentially objectionable scenes, language, or other content. The second memory reader provides a convenient medium for the loading of filter information, whether data files, executable program code, or the like, to local memory of the media player to employ during filtered playback. Alternatively, the filters may be accessed from the removable storage media during playback rather than loading to local memory.

Abstract: A media player configured with a first removable memory reader, such as a DVD drive, and a second removable memory reader, such as a flash memory reader, adapted to communicate with a removable memory containing filter data. The media player is configured to allow filtered playback of a multimedia presentation, such as a movie. Filtered playback causes certain portions of the multimedia presentation to be skipped, muted, blurred, cropped, or otherwise modified to eliminate or reduce potentially objectionable scenes, language, or other content. The second memory reader provides a convenient medium for the loading of filter information, whether data files, executable program code, or the like, to local memory of the media player to employ during filtered playback. Alternatively, the filters may be accessed from the removable storage media during playback rather than loading to local memory.

Abstract: A client server arrangement for downloading media content filters from a server device to a client device. The media content filters define portions of a separate audio visual presentation containing potentially objectionable subject matter. Depending on user selections, identified portions of the audio/visual presentation may be skipped and/or muted during play. In one particular implementation, the client device, e.g., a DVD player, is configured to initiate a connection with a server device. Upon successful connection, the server device transmits one or more media content filters to the client device. The client device may be configured to determine whether a particular media content filter is available, to facilitate deletion of some existing media content filters in order to secure adequate memory space, and to ensure that the media player has an active account, before initiating a connection with the server device.

Abstract: A client server arrangement for downloading media content filters from a server device to a client device. The media content filters define portions of a separate audio visual presentation containing potentially objectionable subject matter. Depending on user selections, identified portions of the audio/visual presentation may be skipped and/or muted during play. In one particular implementation, the client device, e.g., a DVD player, is configured to initiate a connection with a server device. Upon successful connection, the server device transmits one or more media content filters to the client device. The client device may be configured to determine whether a particular media content filter is available, to facilitate deletion of some existing media content filters in order to secure adequate memory space, and to ensure that the media player has an active account, before initiating a connection with the server device.

Abstract: The invention overcomes the deficiencies of the prior art by providing antibody compositions having improved affinities for Bacillus anthracis antigens. The compositions have important thereapeutic and diagnostic applications, including treatment or detection of infection by Bacillus anthracis.

Abstract: The invention overcomes the deficiencies of the prior art by providing a rapid approach for isolating binding proteins capable of binding small molecules and peptides via “display-less” library screening. In the technique, libraries of candidate binding proteins, such as antibody sequences, are expressed in soluble form in the periplasmic space of gram negative bacteria, such as Escherichia coli, and are mixed with a labeled ligand. In clones expressing recombinant polypeptides with affinity for the ligand, the concentration of the labeled ligand bound to the binding protein is increased and allows the cells to be isolated from the rest of the library. Where fluorescent labeling of the target ligand is used, cells may be isolated by fluorescence activated cell sorting (FACS). The approach is more rapid than prior art methods and avoids problems associated with the surface-expression of ligand fusion proteins employed with phage display.

Abstract: The invention overcomes the deficiencies of the prior art by providing a rapid approach for isolating polypeptides capable of anchoring heterologous polypeptides to a bacterial inner membrane. In the technique, libraries of candidate anchor polypeptides are expressed as fusions with a heterologous polypeptide that is capable of being detected when bound to the inner membrane. In bacteria expressing a functional anchor sequence, the heterologous polypeptide becomes bound to outer face of the inner membrane. Bacteria with the functional anchor sequence can be identified by removing the outer membrane to remove non-anchored heterologous polypeptide followed by detection of anchored heterologous polypeptide. Such bacteria may be detected in numerous ways, including use of direct fluorescence or secondary antibodies that are fluorescently labeled, allowing use of efficient techniques such as fluorescence activated cell sorting (FACS).