Abstract: A calibrator bottle and method of calibration. The calibrator bottle includes a plastic material that is doped or compounded with a fluorescent material system that fluoresces in a target range and may be used to calibrate a blood culture instrument that measures sample fluorescence to determine bottle positivity or negativity. Also described herein is a system and method to control the temperature of samples in a blood culture apparatus using a resistance temperature device (RTD) device that measures the temperature of the sample. The sample measurements are correlated with the ambient temperature to determine an ambient temperature that will provide a target bottle content temperature.

Abstract: A system that may automatically and rapidly process blood culture bottles. Such processing includes obtaining an image of a label placed on the cylindrical surface of the blood culture bottle. The system may also determine an amount of blood sample that has been added to the blood culture bottle by comparing a sample level to pre-placed fiducial. The distance between the liquid meniscus in the blood culture bottle and fiducial may be used to determine blood volume. The imaging apparatus may also detect internal conditions of the blood culture bottle such as the presence of foam or the presence of culture media in a neck portion of the blood culture bottle. The image of the label is obtained from one or more images captured by a camera or scanner. The information on the label may be read automatically and therefore the blood culture bottles do not have to be processed manually, increasing throughput of the apparatus.

Abstract: An apparatus for storing and monitoring blood culture bottles. The apparatus has a moveable rack configured as a drum having a plurality of receptacles therein for receiving blood culture bottles. The drum is disposed in a housing. The housing includes a heater and a blower for incubating the blood culture bottles at elevated temperatures. Optionally the apparatus has a plurality of drums, each having a plurality of receptacles for receiving blood culture bottles.

Abstract: A multi-port disposable device that can both vent a sealed culture vessel and draw a sample therefrom into a collection vessel. The multi-port disposable device has a first port configured to receive the top portion of a culture vessel and attach thereto. The multi-port disposable device has a second port configured to receive a sample collection vessel. The multi-port disposable device has first and second needles. The first needle has a cannula configured to penetrate a septum or cap of the culture vessel. Optionally, the cannula terminates in a layer of foam that carries a microbial agent such that any vapor that is transmitted from the culture vessel through the cannula when venting the sealed culture vessel is absorbed by the foam. Optionally, the ports of the disposable device are configured as sleeves. The sleeve of the first port receives a portion of the culture vessel and the sleeve of the second port receives the collection vessel.

Abstract: A method and automated apparatus for locating and selecting a colony of microorganisms on a culture dish and subjecting the obtained sample to a plurality of downstream tests including a test to identify the microorganism and a test to identify the susceptibility of the microorganism to antibiotics. The method includes the automated steps of locating and selecting a colony of microorganisms on a culture dish; obtaining a sample of the selected colony of microorganisms; preparing a suspension of a sample of microorganisms automatically by submerging the pick tool with the sample in a suspension, after which the pick tool is vibrated in at least the vertical direction to release the sample from the pick tool in the suspension. The turbidity of the suspension is monitored to ensure that the concentration of microorganism in suspension is sufficient so that the suspension is used a source for sample for both identification and antibiotic susceptibility of the microorganisms in the sample.

Abstract: A method and automated apparatus for locating and selecting a colony of microorganisms on a culture dish and subjecting the obtained sample to a plurality of downstream tests including a test to identify the microorganism and a test to identify the susceptibility of the microorganism to antibiotics. The method includes the automated steps of locating and selecting a colony of microorganisms on a culture dish; obtaining a sample of the selected colony of microorganisms; preparing a suspension of a sample of microorganisms automatically by submerging the pick tool with the sample in a suspension, after which the pick tool is vibrated in at least the vertical direction to release the sample from the pick tool in the suspension. The turbidity of the suspension is monitored to ensure that the concentration of microorganism in suspension is sufficient so that the suspension is used a source for sample for both identification and antibiotic susceptibility of the microorganisms in the sample.

Abstract: A customizable laboratory instrument comprising an interior space for receipt of test materials therein. An indented exterior surface of the laboratory instrument defines a boundary surface, a backing surface, and a sidewall. The backing surface is offset from the boundary surface a distance substantially equal to a depth of the sidewall. A panel having an edge is attachable to the sidewall such that a space is formed between the panel and backing surface for receipt of an object therein.

Abstract: A method and automated apparatus for locating and selecting a colony of microorganisms on a culture dish and subjecting the obtained sample to a plurality of downstream tests including a test to identify the microorganism and a test to identify the susceptibility of the microorganism to antibiotics. The method includes the automated steps of locating and selecting a colony of microorganisms on a culture dish; obtaining a sample of the selected colony of microorganisms; preparing a suspension of a sample of microorganisms automatically by submerging the pick tool with the sample in a suspension, after which the pick tool is vibrated in at least the vertical direction to release the sample from the pick tool in the suspension. The turbidity of the suspension is monitored to ensure that the concentration of microorganism in suspension is sufficient so that the suspension is used a source for sample for both identification and antibiotic susceptibility of the microorganisms in the sample.

Abstract: A customizable laboratory instrument comprising an interior space for receipt of test materials therein. An indented exterior surface of the laboratory instrument defines a boundary surface, a backing surface, and a sidewall. The backing surface is offset from the boundary surface a distance substantially equal to a depth of the sidewall. A panel having an edge is attachable to the sidewall such that a space is formed between the panel and backing surface for receipt of an object therein.