Abstract: Provided herein are compositions and methods related to probe-based genotyping, such as pharmacogenomic genotyping.

Abstract: Provided by the inventive concept is a DNA methylation-based platform, and machine learning algorithms, for diagnosing respiratory pathogens including SARS-CoV-2 and predicting COVID-19 related outcomes, and methods of using the same, such as in identifying the presence of a viral infection, such as a SARS-CoV-2 infection, determining whether a subject has COVID-19, and/or whether a subject with COVID-19 is likely to develop acute respiratory distress syndrome or multisystem inflammatory syndrome in children.

Abstract: While methylation chips have been widely used in human studies over the last ten years, methylation chips for non-human species have not, perhaps due to lack of sufficient demand and/or because species specific methylation chips may be suboptimal for cross-species comparisons. To address challenges in this technology, we developed an algorithm, Conserved Methylation Array Probe Selector (CMAPS), which repurposes the degenerate base technology used to tolerate within-human variation to tolerate cross-species mutations. CMAPS performs a greedy search to obtain a maximal number of species that can be targeted using a probe for any CpG in the human genome, based on a multiple sequence alignment. CMAPS then ranks all the probes and chooses a final set so that arrays can be made that can query a large number of mammalian species and varied genomic positions based on external annotations of exons, CpG islands and hyper versus hypo methylated regions.

Abstract: Provided herein is a wheeled device useful for transporting and storing a wide variety of cargos, including cartons, crates, boxes and the like and which finds special utility in transporting and stowing musical equipment, such as amplifiers and speakers. A device according to the invention includes vertical and horizontal elements, and further includes support arms having a variable width by which the cargo may be grasped and firmly held in place on the device. Use of a device according to the invention causes optimization of floor space required to store items on a moveable base.