Abstract: A method and a system for introducing a sample into a mobile phase of a chromatography system is provided. The method includes initially directing the mobile phase directly into a separation unit of the chromatography system, bypassing a sample loop, the mobile phase including a combined solvent, metered from a pressurized first solvent and a second solvent; loading the sample into the sample loop, while the mobile phase continues to be directed directly into the separation unit; pressurizing the sample in the sample loop with the pressurized first solvent, while the mobile phase continues to be directed directly into the separation unit; and switching the sample loop into the mobile phase, thereby introducing the pressurized sample to the separation unit.

Abstract: A method and a system for introducing a sample into a mobile phase of a chromatography system is provided. The method includes initially directing the mobile phase directly into a separation unit of the chromatography system, bypassing a sample loop, the mobile phase including a combined solvent, metered from a pressurized first solvent and a second solvent; loading the sample into the sample loop, while the mobile phase continues to be directed directly into the separation unit; pressurizing the sample in the sample loop with the pressurized first solvent, while the mobile phase continues to be directed directly into the separation unit; and switching the sample loop into the mobile phase, thereby introducing the pressurized sample to the separation unit.

Abstract: An apparatus and method for filtering a liquid sample. A vessel defines a reservoir. An insert is at least partially and slidably positioned within the reservoir. The insert defines a collection volume, and the collection volume is in fluid communication with the reservoir. A separation element is positioned to partition the collection volume from the reservoir.

Abstract: The subject invention includes methods and devices for separating at least two constituents of a highly aqueous fluid. The subject methods include contacting a highly aqueous fluid with a stationary phase having a low density hydrophobic bonded phase under conditions to separate at least two constituents. Also provided are systems that include a stationary phase having a low density hydrophobic bonded phase, a highly aqueous fluid comprising at least two constituents and a device configured to perform liquid chromatography. Kits for use in practicing the subject methods are also provided.

Abstract: The subject invention includes methods and devices for separating at least two constituents of a highly aqueous fluid. The subject methods include contacting a highly aqueous fluid with a stationary phase having a low density hydrophobic bonded phase under conditions to separate at least two constituents. Also provided are systems that include a stationary phase having a low density hydrophobic bonded phase, a highly aqueous fluid comprising at least two constituents and a device configured to perform liquid chromatography. Kits for use in practicing the subject methods are also provided.