Abstract: An apparatus for packing a chromatography column with a granular packing medium is disclosed. The apparatus includes a chamber adapted to hold a mixture of the packing medium and a liquid. The chamber is in fluid communication with a pump and the column. A vibrator is positioned so as to agitate the mixture within the chamber. The invention also includes a method of packing the chromatography column by forming a mixture comprising the packing medium and the liquid, positioning the mixture within the chamber, agitating the mixture, and pumping liquid into the chamber thereby forcing the mixture through the column.

Abstract: A system and method for substantially continuous fraction collection includes a control device and a fluidic switch. The control device selects the state of the fluidic switch, and thereby determines which of a plurality of output ports effluent will exit.

Abstract: The present invention provides silica-based reversed-phase HPLC methods that lead to higher retention of the analytes in the column and longer column lifetimes than usually observed under medium to high pH aqueous mobile phase conditions. The inventive methods comprise eluting the HPLC column using an aqueous mobile phase comprising at least one fluorinated additive. Preferred additives are polyfluorinated alcohols such as 2,2,2-trifluoroethanol and 1,1,1,3,3,3-hexafluoroisopropanol. The methods of the present invention may be used for analyzing, separating, purifying, and/or isolating small organic molecules, natural products, as well as biomolecules such as polypeptides, oligonucleotides and polynucleotides (e.g., DNA fragments).

Abstract: Compositions include stationary phases that include at least one bonded phase, where the bonded phase includes a silicon atom directly attached to (1) at least one bulky group, and (2) a long chain with an embedded polar group where in certain embodiments the embedded polar group is carbonate, carbamate, urea, ether, or amide. In certain embodiments, the bulky group is an alkyl or an aryl such as isopropyl or isobutyl. The compositions may be contacted with a mobile phase under conditions sufficient to separate the at least two constituents. Also provided are systems and kits for use with the subject methods.

Abstract: The present invention provides silica-based reversed-phase HPLC methods that lead to higher retention of the analytes in the column and longer column lifetimes than usually observed under medium to high pH aqueous mobile phase conditions. The inventive methods comprise eluting the HPLC column using an aqueous mobile phase comprising at least one fluorinated additive. Preferred additives are polyfluorinated alcohols such as 2,2,2-trifluoroethanol and 1,1,1,3,3,3-hexafluoroisopropanol. The methods of the present invention may be used for analyzing, separating, purifying, and/or isolating small organic molecules, natural products, as well as biomolecules such as polypeptides, oligonucleotides and polynucleotides (e.g., DNA fragments).