Abstract: Described herein are methods, compositions, and systems for multiplex editing using Cas enzymes or editing of T-cells or related cells using Cas enzymes.

Abstract: Described herein are methods, compositions, and systems derived from uncultivated microorganisms useful for gene editing.

Abstract: The present disclosure provides for endonuclease enzymes as well as methods of using such enzymes or variants thereof.

Abstract: The present disclosure provides systems and methods for transposing a cargo nucleotide sequence to a target nucleic acid site. These systems and methods may comprise a first double-stranded nucleic acid comprising the cargo nucleotide sequence, wherein the cargo nucleotide sequence is configured to interact with a retrotransposase, and the retrotransposase, wherein said retrotransposase is configured to transpose the cargo nucleotide sequence to the target nucleic acid site.

Abstract: The present disclosure provides for endonuclease enzymes having HEPN domains, as well as methods of using such enzymes or variants thereof.

Abstract: The present disclosure provides for endonuclease enzymes having distinguishing domain features, as well as methods of using such enzymes or variants thereof.

Abstract: Described herein are methods, compositions, and systems derived from uncultivated microorganisms useful for gene editing.

Abstract: The present disclosure provides systems and methods for transposing a cargo nucleotide sequence to a target nucleic acid site. These systems and methods may comprise a first double-stranded nucleic acid comprising the cargo nucleotide sequence, wherein the cargo nucleotide sequence is configured to interact with a transposase, and the transposase, wherein said transposase is configured to transpose the cargo nucleotide sequence to the target nucleic acid site.

Abstract: The present disclosure provides for endonuclease enzymes having distinguishing domain features, as well as methods of using such enzymes or variants thereof.

Abstract: The present disclosure provides for endonuclease enzymes as well as methods of using such enzymes or variants thereof.

Abstract: The present disclosure provides systems and methods for transposing a cargo nucleotide sequence to a target nucleic acid site. These systems and methods can comprise a nucleic acid comprising the cargo nucleotide sequence, wherein the cargo nucleotide sequence is configured to interact with a retrotransposase, and the retrotransposase, wherein said retrotransposase is configured to transpose the cargo nucleotide sequence to the target nucleic acid site. The systems and methods can also involve use of functional fragments of retrotransposases.

Abstract: The present disclosure provides for endonuclease enzymes having distinguishing domain features, as well as methods of using such enzymes or variants thereof.

Abstract: The present disclosure provides for endonuclease enzymes having distinguishing domain features, as well as methods of using such enzymes or variants thereof.

Abstract: Disclosed herein are engineered nucleases and nuclease systems, including chimeric nucleases and chimeric nuclease systems. Engineered and chimeric nucleases disclosed herein include nucleic acid guided nuclease. Additionally disclosed herein are methods of generating engineered nucleases and methods of using the same.

Abstract: The present disclosure provides for endonuclease enzymes having distinguishing domain features, as well as methods of using such enzymes or variants thereof.

Abstract: Described herein are methods, compositions, and systems derived from uncultivated microorganisms useful for gene editing.