Patents by Inventor Brian D. Mariani

Brian D. Mariani has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Methods and compositions for detecting Zika virus
    Patent number: 10626473
    Abstract: Provided herein is a highly sensitive and robust method for Zika detection in semen, as well as related compositions. The method can include: (a) extracting nucleic acids from a human semen sample; (b) detecting Zika virus nonstructural protein 5 (NS5) mRNA using real-time reverse-transcription polymerase chain reaction (rRT-PCR); and (c) simultaneously, in the rRT-PCR, detecting human beta-actin mRNA as positive control.
    Type: Grant
    Filed: October 3, 2017
    Date of Patent: April 21, 2020
    Assignee: Genetics & IVG Institute, Inc.
    Inventors: Brian D. Mariani, Harvey J. Stern
  • METHODS AND COMPOSITIONS FOR DETECTING ZIKA VIRUS IN SEMEN
    Publication number: 20180142310
    Abstract: Provided herein is a highly sensitive and robust method for Zika detection in semen, as well as related compositions. The method can include: (a) extracting nucleic acids from a human semen sample; (b) detecting Zika virus nonstructural protein 5 (NS5) mRNA using real-time reverse-transcription polymerase chain reaction (rRT-PCR); and (c) simultaneously, in the rRT-PCR, detecting human beta-actin mRNA as positive control.
    Type: Application
    Filed: October 3, 2017
    Publication date: May 24, 2018
    Inventors: Brian D. Mariani, Harvey J. Stern
  • Amplification based detection of bacterial infection
    Patent number: 5654141
    Abstract: A method of detecting a bacterial infection in a patient comprising obtaining a patient sample; obtaining a sample of nucleic acids from the patient sample; separating charged cellular components from the nucleic acids using a mixed bed ion exchange resin; amplifying bacterial nucleic acids in the sample; and detecting the presence or absence of amplified nucleic acids wherein the presence of amplified nucleic acids indicates a bacterial infection.
    Type: Grant
    Filed: November 18, 1994
    Date of Patent: August 5, 1997
    Assignee: Thomas Jefferson University
    Inventors: Brian D. Mariani, Rocky S. Tuan