Patents by Inventor Brian H. Johnston

Brian H. Johnston has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 12268207
    Abstract: Methods, systems, and compositions are provided for extracting bone marrow cells from bone obtained from deceased donors, for preparing the bone marrow for cryopreservation, and for obtaining desired cells from cryopreserved and fresh bone marrow. In some embodiments, the methods provide cryopreserved compositions having increased viability of bone marrow cells.
    Type: Grant
    Filed: July 14, 2023
    Date of Patent: April 8, 2025
    Assignee: OSSIUM HEALTH, INC.
    Inventors: Erik J. Woods, Brian H. Johnstone, Dongsheng Gu, Aubrey Marie Sherry, Kelsey Gwen Musall
  • Publication number: 20250034528
    Abstract: The present disclosure provides composition and methods preparing a population of isolated MSCs for pharmaceutical application, the method comprising: providing a population of isolated MSCs; and synchronizing the population of isolated MSCs, wherein no more than 25% of the population of isolated MSCs are in the S phase of the cell cycle.
    Type: Application
    Filed: October 11, 2024
    Publication date: January 30, 2025
    Inventors: Erik J. Woods, Brian H. Johnstone, Dongsheng Gu, Jianguang Du
  • Publication number: 20240344056
    Abstract: Disclosed herein are compositions and methods for the detection of DNAs in a sample, including single-stranded DNA, denatured double-stranded DNAs and DNA fragments for research and clinical diagnostic purposes. The methods and compositions disclosed herein may be used for preparing next generation sequencing libraries of highly fragmented DNA molecules isolated from biofluids (such as plasma, serum, saliva and urine), FFPE samples and ancient organisms. The methods involve the ligation of hairpin adaptors to the 3?-end Sand the 5?-end of the ssDNA.
    Type: Application
    Filed: June 2, 2022
    Publication date: October 17, 2024
    Inventors: Sergei A. Kazakov, Ryan E. Hogans, Colin Hortman, Yolanda F.A. Townsley, Rachel Harbeitner, Brian H. Johnston
  • Patent number: 11964997
    Abstract: Disclosed herein are methods and compositions for the detection of small RNAs in a sample. The methods and compositions disclosed herein may be used for preparing sequencing libraries of the small RNAs, fragments of RNAs and DNAs.
    Type: Grant
    Filed: March 12, 2021
    Date of Patent: April 23, 2024
    Assignee: RealSeq Biosciences, Inc.
    Inventors: Sergei A. Kazakov, Sergio Barberan-Soler, Anne Dallas, Brian H. Johnston
  • Patent number: 11896005
    Abstract: The present disclosure relates to, at least, a vacuum-assisted method for infiltrating cadaver bone with a cryoprotectant and a method for rapidly warming the cryopreserved cadaver bone for bone marrow processing.
    Type: Grant
    Filed: January 17, 2023
    Date of Patent: February 13, 2024
    Assignee: Ossium Health, Inc.
    Inventors: Erik J. Woods, Brian H. Johnstone
  • Patent number: 11786558
    Abstract: Described herein are methods of cell therapies. Also described herein are methods of generating donor derived T cells in an organ transplant recipient, by administering bone marrow stem cells to the organ transplant recipient about 1 to about 30 days after the organ transplant recipient receives one or more organ transplants.
    Type: Grant
    Filed: March 16, 2022
    Date of Patent: October 17, 2023
    Assignee: Ossium Health, Inc.
    Inventors: Erik J. Woods, Brian H. Johnstone, Dongsheng Gu, Aubrey Marie Sherry, Kelsey Gwen Musall, Megan Sykes, Tomoaki Kato, Jianing Fu
  • Patent number: 11744243
    Abstract: Methods, systems, and compositions are provided for extracting bone marrow cells from bone obtained from deceased donors, for preparing the bone marrow for cryopreservation, and for obtaining desired cells from cryopreserved and fresh bone marrow.
    Type: Grant
    Filed: March 1, 2022
    Date of Patent: September 5, 2023
    Assignee: OSSIUM HEALTH, INC.
    Inventors: Erik J. Woods, Brian H. Johnstone, Dongsheng Gu, Aubrey Marie Sherry, Kelsey Gwen Musall
  • Patent number: 11702637
    Abstract: Methods are provided for extracting bone marrow cells from bone obtained from deceased donors, for preparing the bone marrow for cryopreservation and for obtaining desired cells from cryopreserved and fresh bone marrow.
    Type: Grant
    Filed: September 4, 2020
    Date of Patent: July 18, 2023
    Assignee: Ossium Health, Inc.
    Inventors: Erik John Woods, Brian H. Johnstone, Dongsheng Gu, Aubrey Marie Sherry, Kelsey Gwen Musall, John R. Woods, James Hardin, Alan Hooks
  • Patent number: 11697799
    Abstract: Methods are provided for extracting bone marrow cells from bone obtained from deceased donors, for preparing the bone marrow for cryopreservation and for obtaining desired cells from cryopreserved and fresh bone marrow.
    Type: Grant
    Filed: January 6, 2020
    Date of Patent: July 11, 2023
    Assignee: Ossium Health, Inc.
    Inventors: Erik John Woods, Brian H. Johnstone, Dongsheng Gu, Aubrey Marie Sherry, Kelsey Gwen Musall, John R. Woods, James Hardin, Alan Hooks
  • Patent number: 11447750
    Abstract: Methods are provided for extracting bone marrow cells from bone obtained from deceased donors, for preparing the bone marrow for cryopreservation and for obtaining desired cells from cryopreserved and fresh bone marrow.
    Type: Grant
    Filed: September 4, 2020
    Date of Patent: September 20, 2022
    Assignee: Ossium Health, Inc.
    Inventors: Erik John Woods, Brian H. Johnstone, Dongsheng Gu, Aubrey Marie Sherry, Kelsey Gwen Musall, John R. Woods, James Hardin, Alan Hooks
  • Publication number: 20220241342
    Abstract: Described herein are methods of cell therapies. Also described herein are methods of generating donor derived T cells in an organ transplant recipient, by administering bone marrow stem cells to the organ transplant recipient about 1 to about 30 days after the organ transplant recipient receives one or more organ transplants.
    Type: Application
    Filed: March 16, 2022
    Publication date: August 4, 2022
    Inventors: Erik J. Woods, Brian H. Johnstone, Dongsheng Gu, Aubrey Marie Sherry, Kelsey Gwen Musall, Megan Sykes, Tomoaki Kato
  • Publication number: 20220220484
    Abstract: Wound healing is a complex homeostatic process in which several distinct types coordinate to repair a physical damage. Failure to close wounds contributes to the pathology of conditions like diabetes mellitus, particularly in the elderly. Presented herein are molecules, pharmaceutical compositions, and methods for applying small RNA oligonucleotide technology to wound healing. Small RNA oligonucleotide approaches as disclosed herein provide a therapeutic strategy for improving both basal and pathological wound healing.
    Type: Application
    Filed: March 28, 2022
    Publication date: July 14, 2022
    Inventors: Anne DALLAS, Heini ILVES, Sumedha JAYASENA, Brian H. JOHNSTON
  • Patent number: 11319538
    Abstract: Wound healing is a complex homeostatic process in which several distinct types coordinate to repair a physical damage. Failure to close wounds contributes to the pathology of conditions like diabetes mellitus, particularly in the elderly. Presented herein are molecules, pharmaceutical compositions, and methods for applying small RNA oligonucleotide technology to wound healing. Small RNA oligonucleotide approaches as disclosed herein provide a therapeutic strategy for improving both basal and pathological wound healing.
    Type: Grant
    Filed: September 5, 2019
    Date of Patent: May 3, 2022
    Assignee: SOMAGENICS, INC.
    Inventors: Anne Dallas, Heini Ilves, Sumedha Jayasena, Brian H. Johnston
  • Patent number: 11104882
    Abstract: Methods are provided for extracting bone marrow cells from bone obtained from deceased donors, for preparing the bone marrow for cryopreservation and for obtaining desired cells from cryopreserved and fresh bone marrow.
    Type: Grant
    Filed: September 4, 2020
    Date of Patent: August 31, 2021
    Assignee: Ossium Health, Inc.
    Inventors: Erik John Woods, Brian H. Johnstone, Dongsheng Gu, Aubrey Marie Sherry, Kelsey Gwen Musall, John R. Woods, James Hardin, Alan Hooks
  • Publication number: 20210253625
    Abstract: Disclosed herein are methods and compositions for the detection of small RNAs in a sample. The methods and compositions disclosed herein may be used for preparing sequencing libraries of the small RNAs, fragments of RNAs and DNAs.
    Type: Application
    Filed: March 12, 2021
    Publication date: August 19, 2021
    Inventors: Sergei A. KAZAKOV, Sergio BARBERAN-SOLER, Anne DALLAS, Brian H. JOHNSTON
  • Patent number: 11085024
    Abstract: Methods are provided for extracting bone marrow cells from bone obtained from deceased donors, for preparing the bone marrow for cryopreservation and for obtaining desired cells from cryopreserved and fresh bone marrow.
    Type: Grant
    Filed: September 4, 2020
    Date of Patent: August 10, 2021
    Assignee: Ossium Health, Inc.
    Inventors: Erik John Woods, Brian H. Johnstone, Dongsheng Gu, Aubrey Marie Sherry, Kelsey Gwen Musall, John R. Woods, James Hardin, Alan Hooks
  • Patent number: 11014957
    Abstract: Disclosed herein are methods and compositions for the detection of small RNAs in a sample. The methods and compositions disclosed herein may be used for preparing sequencing libraries of the small RNAs, fragments of RNAs and DNAs.
    Type: Grant
    Filed: December 20, 2016
    Date of Patent: May 25, 2021
    Assignee: REALSEQ BIOSCIENCES, INC.
    Inventors: Sergei A. Kazakov, Sergio Barberan-Soler, Anne Dallas, Brian H. Johnston
  • Patent number: 10995318
    Abstract: Methods are provided for extracting bone marrow cells from bone obtained from deceased donors, for preparing the bone marrow for cryopreservation and for obtaining desired cells from cryopreserved and fresh bone marrow.
    Type: Grant
    Filed: September 4, 2020
    Date of Patent: May 4, 2021
    Assignee: Ossium Health, Inc.
    Inventors: Erik John Woods, Brian H. Johnstone, Dongsheng Gu, Aubrey Marie Sherry, Kelsey Gwen Musall, John R. Woods, James Hardin, Alan Hooks
  • Patent number: 10980547
    Abstract: A bone cutting assembly includes a manually actuated upper cutting element that carries a plurality of cutting blades configured to cut through frozen bone segments. A lower cutting element supports a bone segment to be cut and can include cutting blades aligned with the cutting blades of the upper cutting assembly. A pivoting linkage or a rack and pinion arrangement can be provided between the upper cutting element and a manually operated handle to provide sufficient mechanical advantage or leverage to allow an operator to manually cut through the bone.
    Type: Grant
    Filed: October 23, 2020
    Date of Patent: April 20, 2021
    Assignee: Ossium Health, Inc.
    Inventors: Erik J. Woods, Brian H. Johnstone, Joseph Ingalls
  • Patent number: 10870850
    Abstract: Aspects of the present invention include the production and use of chemically modified RNAi agents (e.g., shRNAs) in gene silencing applications. The chemically modified RNAi agents disclosed herein have reduced immunostimulatory activity, increased serum stability, or both, as compared to a corresponding RNAi agent not having the chemical modification. Compositions containing chemically modified RNAi agents according to aspects of the present invention (including pharmaceutical compositions) and kits containing the same are also provided.
    Type: Grant
    Filed: October 5, 2017
    Date of Patent: December 22, 2020
    Assignee: SOMAGENICS, INC.
    Inventors: Qing Ge, Brian H. Johnston, Mark A. Behlke, Heini Ilves, Anne Dallas