Abstract: The present invention provides methods and compositions for glycan analysis of complex solutions, including proteins and cells in a biological sample. The method includes the preparation of substrates for the capture of proteins and cells for multiplexed analysis. Cells and proteins may be captured by antibody arrays, culture, or direct deposition. The invention further relates to the use of protein and cell glycan analysis in the diagnosis and screening of disease states and disease progression.

Abstract: The invention includes a high-through-put method of screening for a biomarker indicative of a disease, including the identification of a glycan alteration in a protein of a diseased patient. Further, the invention includes methods utilizing a microarray to measure the glycan level of a protein.

Abstract: A nucleic acid probe or a novel set of such probes in a microarray is provided. The probe or probe set is useful in the prognosis of patients with clear cell renal cell carcinoma (CC-RCC), wherein aggressive and non-agressive CC-RCC tumor types are characterized by differential expression profiles of genes that hybridize with one or more of these probes.

Abstract: An apparatus for placing a hydrophobic barrier on an examination slide including a vessel, a printer block having a printing surface to form a hydrophobic barrier on the examination slide in a specified pattern, a slide holder, and a reversible actuator. The invention also includes a method for placing a hydrophobic barrier on an examination slide in a specified pattern.