Abstract: The monitoring and control of bioprocesses is provided. The present disclosure provides the ability to generate generic calibration models, independent of cell line, using inline Raman probes to monitor changes in glucose, lactate, glutamate, ammonium, viable cell concentration (VCC), total cell concentration (TCC) and product concentration. Calibration models were developed from cell culture using two different CHOK1SV GS-KO™ cell lines producing different monoclonal antibodies (mAbs). Developed predictive models, qualified using an independent CHOK1SV GS-KO™ cell line not used in calibration, measured changes in glucose, lactate, ammonium, VCC, and TCC with minor prediction errors over the course of cell culture with minimal cell line dependence. The development of these generic models allows the application of spectroscopic PAT techniques in a clinical manufacturing environment, where processes are typically run once or twice in GMP manufacturing based on a common platform process.

Abstract: Various embodiments of the present disclosure provide a multi-input multi-output energy charging system to generate hydrogen, provide baseload energy to a facility, and provide electrical power to charge electric vehicles (EV). In an embodiment, a charging system includes a solid oxide fuel cell (SOFC) system that generates electricity from one or more fuel inputs. One or more fuel inputs are renewable fuels. The charging system further includes a solid oxide electrolyzer cell (SOEC) system coupled to the SOFC system. The SOEC system generates hydrogen from the electricity received from the SOFC system and water input. The SOFC system facilitates the charging of an electric vehicle, storing charge in a battery, and providing electric power to a load from the generated electricity. The SOEC system facilitates refueling a hydrogen fuel cell vehicle from the generated hydrogen and storing the generated hydrogen in a hydrogen storage vessel.

Abstract: The monitoring and control of bioprocesses is provided. The present disclosure provides the ability to generate generic calibration models, independent of cell line, using inline Raman probes to monitor changes in glucose, lactate, glutamate, ammonium, viable cell concentration (VCC), total cell concentration (TCC) and product concentration. Calibration models were developed from cell culture using two different CHOK1SV GS-KO™ cell lines producing different monoclonal antibodies (mAbs). Developed predictive models, qualified using an independent CHOK1SV GS-KO™ cell line not used in calibration, measured changes in glucose, lactate, ammonium, VCC, and TCC with minor prediction errors over the course of cell culture with minimal cell line dependence. The development of these generic models allows the application of spectroscopic PAT techniques in a clinical manufacturing environment, where processes are typically run once or twice in GMP manufacturing based on a common platform process.

Abstract: Various embodiments of the present disclosure provide a multi-input multi-output energy charging system to generate hydrogen, provide baseload energy to a facility, and provide electrical power to charge electric vehicles (EV). In an embodiment, a charging system includes a solid oxide fuel cell (SOFC) system that generates electricity from one or more fuel inputs. One or more fuel inputs are renewable fuels. The charging system further includes a solid oxide electrolyzer cell (SOEC) system coupled to the SOFC system. The SOEC system generates hydrogen from the electricity received from the SOFC system and water input. The SOFC system facilitates the charging of an electric vehicle, storing charge in a battery, and providing electric power to a load from the generated electricity. The SOEC system facilitates refueling a hydrogen fuel cell vehicle from the generated hydrogen and storing the generated hydrogen in a hydrogen storage vessel.

Abstract: The monitoring and control of bioprocesses is provided. The present disclosure provides the ability to generate generic calibration models, independent of cell line, using inline Raman probes to monitor changes in glucose, lactate, glutamate, ammonium, viable cell concentration (VCC), total cell concentration (TCC) and product concentration. Calibration models were developed from cell culture using two different CHOK1SV GS-KO™ cell lines producing different monoclonal antibodies (mAbs). Developed predictive models, qualified using an independent CHOK1SV GS-KO™ cell line not used in calibration, measured changes in glucose, lactate, ammonium, VCC, and TCC with minor prediction errors over the course of cell culture with minimal cell line dependence. The development of these generic models allows the application of spectroscopic PAT techniques in a clinical manufacturing environment, where processes are typically run once or twice in GMP manufacturing based on a common platform process.

Abstract: Systems and methods identify and trigger actions to improve performance in a role in an organization. A server computer receives input data associated with a performance of a target entity in a role in an organization from a remote device. The server computer also receives, via input to a Graphical User Interface (GUI), a set of weights for a respective set of skills for the role. The server computer computes a metric based on the input data and the weights, the metric representing the performance in the role for the target entity. The server computer identifies an action likely to improve the metric and triggers the action.

Abstract: The monitoring and control of bioprocesses is provided. The present disclosure provides the ability to generate generic calibration models, independent of cell line, using inline Raman probes to monitor changes in glucose, lactate, glutamate, ammonium, viable cell concentration (VCC), total cell concentration (TCC) and product concentration. Calibration models were developed from cell culture using two different CHOK1SV GS-KO™ cell lines producing different monoclonal antibodies (mAbs). Developed predictive models, qualified using an independent CHOK1SV GS-KO™ cell line not used in calibration, measured changes in glucose, lactate, ammonium, VCC, and TCC with minor prediction errors over the course of cell culture with minimal cell line dependence. The development of these generic models allows the application of spectroscopic PAT techniques in a clinical manufacturing environment, where processes are typically run once or twice in GMP manufacturing based on a common platform process.

Abstract: The present disclosure provides methods for improving batch-to-batch consistency of proteins produced by eukaryotic cells, as well as proteins produced thereby.