Abstract: The disclosure relates to electronic reader devices, detection compositions and methods for detecting heavy metals (e.g., lead) in water samples using a simple, rapid and highly selective immunoassay system, including lateral and upward flow assay systems. The immunoassay is designed to detect very low levels of a contaminating analyte such as lead at less than 1 ppb with high specificity and selectivity from any common water source (e.g., tap or drinking water). Advantageously, implementation of the assay requires no formal technical training or expensive equipment or reagents and is provided with a multi-use reader that can be used for 50 samples or more. The immunoassay sample collector systems are consumable (single use) and can be purchased in any desired quantity. Assay results are wirelessly transmitted from the reader using a SafeSpout-designed app., which provides important information based on the test results.

Abstract: The present disclosure relates generally to the materials and methods involving biosensing systems. More specifically, the present disclosure relates to biosensors for making real-time, continuous, and quantitative assessments of analyte concentrations in aqueous environments using oxidases. The present disclosure addresses the need for improved methods and systems for making quantitative assessments of various analytes in a continuous and real-time manner, without the need for expensive and time-consuming laboratory processing.